Design and development of heater control circuit without temperature sensor for monitoring hydrogen in argon

by
A thin film based tin oxide sensor is developed to monitor low levels of hydrogen (concentration ranging from 5 to 75 ppm) in the cover gas plenum of the fast breeder test reactor. The heater and the sensor patterns are integrated on a miniature alumina substrate, and necessary electrical leads are incorporated into it. For proper functioning of the sensor, the heater has to be maintained at a constant temperature of 350 °C. This paper gives an outline of the electronics developed to measure the sensor signal and to control the heater temperature.
The major challenge in this work is that there was no provision for embedding a temperature sensor on the heater surface due to physical constraints. This constrained the maintenance of a constant heater temperature for the proper functioning of the sensor. This led us to develop and demonstrate a heater control circuit without a temperature sensor to maintain a fixed temperature for https://biodas.org/ monitoring hydrogen in argon, and electronics for the above-mentioned circuitry is discussed.

ERC-ESICM guidelines on temperature control after cardiac arrest in adults

  • The aim of these guidelines is to provide evidence‑based guidance for temperature control in adults who are comatose after resuscitation from either in-hospital or out-of-hospital cardiac arrest, regardless of the underlying cardiac rhythm. These guidelines replace the recommendations on temperature management after cardiac arrest included in the 2021 post-resuscitation care guidelines co-issued by the European Resuscitation Council (ERC) and the European Society of Intensive Care Medicine (ESICM).
  • The guideline panel included thirteen international clinical experts who authored the 2021 ERC-ESICM guidelines and two methodologists who participated in the evidence review completed on behalf of the International Liaison Committee on Resuscitation (ILCOR) of whom ERC is a member society. We followed the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) approach to assess the certainty of evidence and grade recommendations. The panel provided suggestions on guideline implementation and identified priorities for future research. The certainty of evidence ranged from moderate to low.
  • In patients who remain comatose after cardiac arrest, we recommend continuous monitoring of core temperature and actively preventing fever (defined as a temperature > 37.7 °C) for at least 72 h. There was insufficient evidence to recommend for or against temperature control at 32-36 °C or early cooling after cardiac arrest. We recommend not actively rewarming comatose patients with mild hypothermia after return of spontaneous circulation (ROSC) to achieve normothermia. We recommend not using prehospital cooling with rapid infusion of large volumes of cold intravenous fluids immediately after ROSC.

Thermal Model of an Omnimagnet for Performance Assessment and Temperature Control

An Omnimagnet is an electromagnetic device that enables remote magnetic manipulation of devices such as medical implants and microrobots. It is composed of three orthogonal nested solenoids with a ferromagnetic core at the center. Electrical current within the solenoids leads to undesired temperature increase within the Omnimagnet. If the temperature exceeds the melting point of the wire insulation, device failure may occur. Thus, a study of heat transfer within an Omnimagnet is a necessity, particularly to maximize the performance of the device. A transient heat transfer model that incorporates all three heat transfer modes is proposed and experimentally validated with an average normalized root-mean-square error of less than 4% (data normalized by temperature in degree celsius). The transient model is not computationally expensive and is applicable to Omnimagnets with different structures. The code is applied to calculate the maximum safe operational time at a fixed input current or the maximum safe input current for a fixed time interval. The maximum safe operational time and maximum safe input current depend on size and structure of the Omnimagnet and the lowest critical temperature of all the Omnimagnet materials. A parametric study shows that increasing convective heat transfer during cooling, and during heating with low input currents, is an effective method to increase the maximum operational time of the Omnimagnet. The thermal model is also presented in a state-space equation format that can be used in a real-time Kalman filter current controller to avoid device failure due to excessive heating.

Integrated Temperature and Position Sensors in a Shape-Memory Driven Soft Actuator for Closed-Loop Control

Soft actuators are a promising option for the advancing fields of human-machine interaction and dexterous robots in complex environments. Shape memory alloy wire actuators can be integrated into fiber rubber composites for highly deformable structures. For autonomous, closed-loop control of such systems, additional integrated sensors are necessary. In this work, a soft actuator is presented that incorporates fiber-based actuators and sensors to monitor both deformation and temperature.
The soft actuator showed considerable deformation around two solid body joints, which was then compared to the sensor signals, and their correlation was analyzed. Both, the actuator as well as the sensor materials were processed by braiding and tailored fiber placement before molding with silicone rubber. Finally, the novel fiber-rubber composite material was used to implement closed-loop control of the actuator with a maximum error of 0.5°.

Surface temperature controls the pattern of post-earthquake landslide activity

The patterns and controls of the transient enhanced landsliding that follows strong earthquakes remain elusive. Geostatistical models can provide clues on the underlying processes by identifying relationships with a number of physical variables. These models do not typically consider thermal information, even though temperature is known to affect the hydro-mechanical behavior of geomaterials, which, in turn, controls slope stability. Here, we develop a slope unit-based multitemporal susceptibility model for the epicentral region of the 2008 Wenchuan earthquake to explore how land surface temperature (LST) relates to landslide patterns over time. 3
We find that LST can explain post-earthquake landsliding while it has no visible effect on the coseismic scene, which is dominated by the strong shaking. Specifically, as the landscape progressively recovers and landslide rates decay to pre-earthquake levels, a positive relationship between LST and landslide persistence emerges. This seems consistent with the action of healing processes, capable of restoring the thermal sensitivity of the slope material after the seismic disturbance. Although analyses in other contexts (not necessarily seismic) are warranted, we advocate for the inclusion of thermal information in geostatistical modeling as it can help form a more physically consistent picture of slope stability controls.

Stuart Temperature Controller SCT1
STI5500 Scientific Laboratory Supplies EACH 342 EUR

Heidolph Electronic Temperature Controller EKT SS Sensor
STI2100 Scientific Laboratory Supplies EACH 779.44 EUR

Eppendorf Centrifuge 5702RH temperature controlled without rotor
E5704000060 Scientific Laboratory Supplies EACH 5428.8 EUR

Eppendorf Centrifuge 5702RH temperature controlled without rotor- IVD Only
E5704000067 Scientific Laboratory Supplies EACH 5701.2 EUR

Temperature Probe 400oc
HEA5262 Scientific Laboratory Supplies EACH 453.6 EUR

Temperature Probe 800oc
HEA5264 Scientific Laboratory Supplies EACH 506.4 EUR

pH/Temperature Electrode
PHM2024 Scientific Laboratory Supplies EACH 195.6 EUR

Temperature Adjustment Set
BAL5042 Scientific Laboratory Supplies EACH 1561.2 EUR

External Temperature Probe
BLO1270 Scientific Laboratory Supplies EACH 300 EUR

External Temperature Probe
BSH-TP1 Benchmark Scientific 1 PC 228 EUR

Optional Temperature Probe
MIX1265 Scientific Laboratory Supplies EACH 183.6 EUR

Temperature probe RS600/900
HEA5518 Scientific Laboratory Supplies EACH 248.4 EUR

Agarose, Low Melt Temperature
40100156-1 Bio-WORLD 10 g 68.53 EUR

Agarose, Low Melt Temperature
40100156-2 Bio-WORLD 25 g 136.81 EUR

Agarose, Low Melt Temperature
40100156-3 Bio-WORLD 50 g 258.28 EUR

Agarose, Low Melt Temperature
40100156-4 Bio-WORLD 100 g 466.78 EUR

IKA PT1000.70 Temperature Probe
STI2837 Scientific Laboratory Supplies EACH 231.6 EUR

pt1000 temperature compensator
ST10N Consort ea 112.8 EUR

pt1000 temperature compensator
ST20N Consort ea 117.6 EUR

Hanna Temperature Probe+Cable
PHM4124 Scientific Laboratory Supplies EACH 122.4 EUR

Temperature probe for compost
THE0810 Scientific Laboratory Supplies EACH 162 EUR

Temperature Datalogger(EBI 300)
THE1846 Scientific Laboratory Supplies EACH 113.59 EUR

Evaluation of a Wearable Non-Invasive Thermometer for Monitoring Ear Canal Temperature during Physically Demanding (Outdoor) Work

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Aimed at preventing heat strain, health problems, and absenteeism among workers with physically demanding occupations, a continuous, accurate, non-invasive measuring system may help such workers monitor their body (core) temperature. The aim of this study is to evaluate the accuracy and explore the usability of the wearable non-invasive Cosinuss° °Temp thermometer. Ear canal temperature was monitored in 49 workers in real-life working conditions. After individual correction, the results of the laboratory and field study revealed high correlations compared to ear canal infrared thermometry for hospital use. After performance of the real-life working tasks, this correlation was found to be moderate.
It was also observed that the ambient environmental outdoor conditions and personal protective clothing influenced the accuracy and resulted in unrealistic ear canal temperature outliers. It was found that the Cosinuss° °Temp thermometer did not result in significant interference during work. Therefore, it was concluded that, without a correction factor, the Cosinuss° °Temp thermometer is inaccurate. Nevertheless, with a correction factor, the reliability of this wearable ear canal thermometer was confirmed at rest, but not in https://biodas.org/ outdoor working conditions or while wearing a helmet or hearing protection equipment.

Mobile Health-Based Thermometer for Monitoring Wound Healing After Endovascular Therapy in Patients With Chronic Foot Ulcer: Prospective Cohort StudY

Background: Foot temperature may increase after endovascular therapy, but the relationship between foot temperature and wound healing is unclear.
Objective: This study was performed to evaluate the feasibility of a mobile health (mHealth)-based thermometer for foot temperature monitoring in patients with chronic foot ulcer before and after endovascular therapy and to determine the association between temperature change and wound healing time.
Methods: This was a prospective cohort study. Patients who had a chronic foot ulcer (>3 months) and underwent endovascular therapy between June 2019 and December 2019 were included. The participants received standard medical care and endovascular therapy for revascularization. The mHealth-based thermometer, composed of 4 temperature-sensing chips, was put on the foot before and after endovascular therapy. Data from the chips were transferred to an associated mobile phone app via Bluetooth. Wound healing time was estimated using the Kaplan-Meier method, and the associations between baseline characteristics and clinical outcomes were evaluated using a Cox proportional hazard model.
Results: A total of 163 patients with chronic foot ulcer who underwent endovascular therapy were enrolled and followed up until wound healing was complete or for 180 days. The mean foot temperature before endovascular therapy was 30.6 (SD 2.8 °C). Foot temperature increased significantly (mean 32.1 °C, SD 2.8 °C; P=.01) after the procedure. Wound healing time was significantly different in the Kaplan-Meier curves of the patient group with temperature changes ≥2 °C and the group with temperature changes ≤2 °C (log-rank P<.001). A foot temperature increase ≥2 °C after endovascular therapy was associated with increased wound healing in univariate analysis (hazard ratio [HR] 1.78, 95% CI 1.24-2.76, P=.02), and the association remained significant in multivariate analysis (HR 1.69, 95% CI 1.21-2.67, P=.03).
Conclusions: The mHealth-based thermometer was feasible and useful for foot temperature monitoring, which may provide health care professionals with a new endpoint for endovascular therapy. Foot temperature increases ≥2 °C after endovascular therapy were associated with faster wound healing in patients with chronic foot ulcer. Further studies are needed, however, to confirm these findings.

Tympanic thermometers support fast and accurate temperature monitoring in acute and alternative care

This article explores body temperature and the physiological process of thermoregulation. Normal body temperature and body temperature changes are discussed, including comorbidities associated with body temperature and signs of hyperthermia and hypothermia, and the factors that affect intraoperative temperature regulation.
The evidence base behind thermometry is discussed and is applied to contemporary clinical conditions and symptoms, including: sepsis and suspected COVID-19. After discussing clinical considerations and regulations that encompass thermometry, three case studies present the use of the Genius 3 Tympanic Thermometer in clinical practice, with user feedback supporting its benefits, which include speed, accuracy and ease of use.

Mitochondria-Anchored Molecular Thermometer Quantitatively Monitoring Cellular Inflammations

Temperature in mitochondria can be a critical indicator of cell metabolism. Given the highly dynamic and inhomogeneous nature of mitochondria, it remains a big challenge to quantitatively monitor the local temperature changes during different cellular processes. To implement this task, we extend our strategy on mitochondria-anchored thermometers from “on-off” probe Mito-TEM to a ratiometric probe Mito-TEM 2.0 based on the Förster resonance energy transfer mechanism. Mito-TEM 2.0 exhibits not only a sensitive response to temperature through the ratiometric changes of dual emissions but also the specific immobilization in mitochondria via covalent bonds.
Both characters support accurate and reliable detection of local temperature for a long time, even in malfunctioning mitochondria. By applying Mito-TEM 2.0 in fluorescence ratiometric imaging of cells and zebrafishes, we make a breakthrough in the quantitative visualization of mitochondrial temperature rises in different inflammation states.

Non-invasive and wearable thermometer for continuous monitoring of core body temperature under various convective conditions

We describe the design of a thermometer that can be worn during everyday activities for monitoring core body temperature (CBT) at the skin surface. This sensor estimates the CBT by measuring the heat flux from the body core based on a thermal conductive model. The heat flux is usually affected by the ambient convective conditions (e.g. air conditioner or posture), which in turn affects the model’s accuracy. Thus, we analytically investigated heat conduction and designed a sensor interface that would be robust to convection changes. We performed an in vitro experiment and a preliminary in vivo experiment. The accuracy of CBT in an in vitro experiments was 0.1°C for convective values ranging from 0 to 1.2 m/s. The wearable thermometer has high potential as non-invasive CBT monitor.

Effect of monitoring the onset of calving by a calving alarm thermometer on the prevalence of dystocia, stillbirth, retained fetal membranes and clinical metritis in a Hungarian dairy farm

The objective of the present study was to assess the effectiveness of an intravaginal thermometer in the field prediction of the second stage of labor and to determine its impact on the health of dams and newborn calves. Holstein cows (n = 241) were randomly selected about 5 (mean ± SD: 4.7 ± 2.0) days before the expected date of calving and the thermometer was inserted into the vagina. Another 113 cattle served as controls. There was no false alarm during the experiment. The risk of dystocia (Score >1) was 1.9 times higher, the prevalence of stillbirth was 19.8 times higher, the risk of retained fetal membranes (RFM) was 2.8 times higher and the risk of clinical metritis was 10.5 times higher in the control group than in the experimental group.
The prevalence of stillbirth was 7 times higher in cows with dystocia compared to cows with eutocia. The presence of dystocia and stillbirth increased the risk of RFM 4 and 5 times, respectively. The occurrence of RFM increased the risk of development of clinical metritis with a 22 times higher odds. The results indicate that the use of calving alert systems not only facilitates controlling the time of parturition and providing prompt and appropriate calving assistance but also decreases the number of dystocia cases and improves reproductive efficiency, postpartum health of the dam and newborn calf survival.

SOM02.0 Selected Ion Monitoring 2 Components
CLPS-SOM-ISB Scientific Laboratory Supplies 1ML 118.8 EUR

Thermometer (TDC 150)
THE1802 Scientific Laboratory Supplies EACH 49.35 EUR

scanning thermometer
T8710 Consort ea 1538.4 EUR

scanning thermometer
T8720 Consort ea 1538.4 EUR

Thermometer/hygrometer
DD98463 Scientific Laboratory Supplies EACH 26.4 EUR

Thermometer -1 to 101C (0.2)
THE1372 Scientific Laboratory Supplies EACH 25.09 EUR

Thermometer -1 to 101C (0.2)
THE1374 Scientific Laboratory Supplies EACH 25.09 EUR

Testo Thermometer 110+Battery
THE2070 Scientific Laboratory Supplies EACH 144.92 EUR

Teflon Thermometer -20 to 110
THE1164 Scientific Laboratory Supplies EACH 21.96 EUR

Teflon Thermometer -10 to 210
THE1166 Scientific Laboratory Supplies EACH 26.76 EUR

Teflon Thermometer -20 to 150
THE1178 Scientific Laboratory Supplies EACH 23.54 EUR

Alarm Thermometer -50 to +150C
THE1502 Scientific Laboratory Supplies EACH 34.8 EUR

Core Thermometer (TFX410)
THE1816 Scientific Laboratory Supplies EACH 309.43 EUR

Spirit Thermometer -10 to 110C
THE1062 Scientific Laboratory Supplies EACH 4.73 EUR

Spirit Thermometer -10 to 150C
THE1064 Scientific Laboratory Supplies EACH 3.92 EUR

Spirit Thermometer -0 to 240F
THE1066 Scientific Laboratory Supplies EACH 4.73 EUR

Spirit Thermometer -10 to 110C
THE1080 Scientific Laboratory Supplies EACH 4.73 EUR

Digital Thermometer -40 to 240
THE1174 Scientific Laboratory Supplies EACH 31.46 EUR

Digital Thermometer -50 to 300
THE1176 Scientific Laboratory Supplies EACH 59.67 EUR

Testo Thermometer 925 K Type
THE2090 Scientific Laboratory Supplies EACH 130.56 EUR

H-B Durac Thermometer
BAB602150000-1EA Scientific Laboratory Supplies EACH 186 EUR

Core Thermometer (TTX 110)
THE1810 Scientific Laboratory Supplies EACH 77.03 EUR

Testo Stick Thermometer 905 T1
THE2020 Scientific Laboratory Supplies EACH 91.39 EUR

Folding thermometer white
THE4532 Scientific Laboratory Supplies EACH 61.36 EUR

An overview of different homogenizers, their working mechanisms and impact on processing of fruits and vegetables

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Fruits and vegetables (F&V) are the second highest recommended foods, rich in antioxidants, vitamins and minerals, vital for building immunity against chronic diseases. F&V processing involves particle size reduction, for which different types of homogenizers, categorized as mechanical homogenizers, pressure homogenizers and ultrasonic homogenizers are used. The review discusses different types of homogenizers, their working mechanism, and application in F&V processing. Among mechanical homogenizers, knife mills are used for primary size reduction, ball mills for the micronization of dried F&V and rotor-stator homogenizers for emulsification.
Use of the ultrasonic homogenizer is limited to extraction of bioactive compounds or as a pre-treatment for dehydration of F&V. High-pressure homogenizers are most widely used and reported due to the synergistic effect of homogenization and temperature increase, resulting in longer shelf-life and better physicochemical properties of the product. Additionally, the review also explains the effect of homogenization on the physicochemical, sensory and https://biodas.org/ nutraceutical properties of the product.

Pre-processing tissue specimens with a tissue homogenizer: clinical and microbiological evaluation

Background: Tissues are valuable specimens in diagnostic microbiology because they are often obtained by invasive methods, and effort should thus be taken to maximize microbiological yield. The objective of this study was to evaluate the added value of using tissue pre-processing (tissue homogenizer instrument gentleMACS Dissociator) in detecting microorganisms responsible for infections.
Methods: We included 104 randomly collected tissue samples, 41 (39.4 %) bones and 63 (60.6 %) soft tissues, many of those (42/104 (40.4 %)) were of periprosthetic origins. We compared the agreement between pre-processing tissues using tissue homogenizer with routine microbiology diagnostic procedure, and we calculated the performance of these methods when clinical infections were used as reference standard.
Results: There was no significant difference between the two methods (McNemar test, p = 0.3). Among the positive culture using both methods (n = 62), 61 (98.4 %) showed at least one similar microorganism. Exactly similar microorganisms were found in 42/62 (67.7 %) of the samples. From the included tissues, 55/ 104 (52.9 %) were deemed as infected. We found that the sensitivity of homogenized tissue procedure was lower (83.6 %) than when tissue was processed using tissue homogenizer (89.1 %). Sub-analysis on periprosthetic tissues and soft or bone tissues showed comparable results.
Conclusions: The added value of GentleMACS Dissociator tissue homogenizer is limited in comparison to routine tissue processing.

Functionality of MC88- and MPC85-Enriched Skim Milk: Impact of Shear Conditions in Rotor/Stator Systems and High-Pressure Homogenizers on Powder Solubility and Rennet Gelation Behavior

  • Milk protein concentrate (MPC) and micellar casein (MC) powders are commonly used to increase the protein concentration of cheese milk. However, highly-concentrated milk protein powders are challenging in terms of solubility. The research question was whether and how incompletely dissolved agglomerates affect the protein functionality in terms of rennet gelation behavior. For the experiments, skim milk was enriched with either MC88 or MPC85 to a casein concentration of 4.5% (w/w) and sheared on a laboratory and pilot scale in rotor/stator systems (colloid mill and shear pump, respectively) and high-pressure homogenizers.
  • The assessment criteria were on the one hand particle sizes as a function of shear rate, and on the other hand, the rennet gelation properties meaning gelling time, gel strength, structure loss upon deformation, and serum loss. Furthermore, the casein, whey protein, and casein macropeptide (CMP) recovery in the sweet whey was determined to evaluate the shear-, and hence, the particle size-dependent protein accessibility. We showed that insufficient powder rehydration prolongs the rennet gelation time, leading to softer, weaker gels, and to lower amounts of CMP and whey protein in the sweet whey.

Characterization of Astaxanthin Nanoemulsions Produced by Intense Fluid Shear through a Self-Throttling Nanometer Range Annular Orifice Valve-Based High-Pressure Homogenizer

Stable, oil-in-water nanoemulsions containing astaxanthin (AsX) were produced by intense fluid shear forces resulting from pumping a coarse reagent emulsion through a self-throttling annular gap valve at 300 MPa. Compared to crude emulsions prepared by conventional homogenization, a size reduction of over two orders of magnitude was observed for AsX-encapsulated oil droplets following just one pass through the annular valve. In krill oil formulations, the mean hydrodynamic diameter of lipid particles was reduced to 60 nm after only two passes through the valve and reached a minimal size of 24 nm after eight passes.
Repeated processing of samples through the valve progressively decreased lipid particle size, with an inflection in the rate of particle size reduction generally observed after 2-4 passes. Krill- and argan oil-based nanoemulsions were produced using an Ultra Shear Technology™ (UST™) approach and characterized in terms of their small particle size, low polydispersity, and stability.

Characteristics of an Emulsion Obtained Using Hydrophobic Hydroxypropyl Methylcellulose as an Emulsifier and a High-Pressure Homogenizer

Hydrophobically modified hydroxypropyl methylcellulose (HM-HPMC), a polymer in which a small amount of HPMC is stearoxyl substituted, was used as an emulsifier of emulsion-type lotion. A high-pressure homogenizer (microfluidizer) was used. The viscosity of the 1% HM-HPMC aqueous gel decreased after passing through the microfluidizer from 5.5 to 2.7 Pa·s. When liquid paraffin (LP) was used as the oil phase, a stable emulsion was obtained with an LP ratio of 1-40%. The apparent viscosity decreased with LP ratios up to 20%, and then increased with increasing LP concentration.
The emulsions with an LP ratio <20% presented a pseudo-viscous flow, similar to that of the diluted polymer solution. HM-HPMC likely adsorbed onto the oil with a stearoxyl group; thus, the interaction between the stearoxyl group, which explained the high viscosity of HM-HPMC, decreased, reducing the viscosity of the emulsion. The LP ratio was 40%, and the emulsion presented a plastic flow, which is typical of concentrated emulsions. The size of the droplet in the emulsion was approximately 1 µm regardless of the LP ratio. When low-viscosity LPs or monoester-type oils such as isopropyl myristate were used, some of the emulsions presented creaming. An emulsion using HM-HPMC as an emulsifier and an appropriate oil homogenized with a microfluidizer is stable, has low viscosity, and can be easily spread on skin.

Proteomic evaluation of plasma membrane fraction prepared from mouse liver and kidney using a bead homogenizer: Enrichment of drug-related transporter proteins

Quantifying the protein levels of drug transporters in plasma membrane fraction helps elucidate the function of these transporters. In this study, we conducted a proteomic evaluation of enriched drug-related transporter proteins in plasma membrane fraction prepared from mouse liver and kidney tissues using the Membrane Protein Extraction Kit and a bead homogenizer. Crude and plasma membrane fractions were prepared using either the Dounce or bead homogenizer, and protein levels were determined using quantitative proteomics.
In liver tissues, the plasma membrane fractions were more enriched in transporter proteins than the crude membrane fractions; the average enrichment ratios of plasma-to-crude membrane fractions were 3.31 and 6.93 using the Dounce and bead homogenizers, respectively. The concentrations of transporter proteins in plasma membrane fractions determined using the bead homogenizer were higher than those determined using the Dounce homogenizer. Meanwhile, in kidney tissues, the plasma membrane fractions were enriched in transporters localized in the brush-border membrane to the same degree for both the homogenizers; however, the membrane fractions obtained using either homogenizer were not enriched in Na+/K+-ATPase and transporters localized in the basolateral membrane. These results indicate that fractionation, using the bead homogenizer, yielded transporter-enriched plasma membrane fractions from mouse liver and kidney tissues; however, no enrichment of basolateral transporters was observed in plasma membrane fractions prepared from kidney tissues.

BeadBug™ Microtube homogenizer, 115V
D1030 Benchmark Scientific 1 each 980.8 EUR

BeadBug™ Microtube homogenizer, 230V
D1030-E Benchmark Scientific 1 PC 980.8 EUR

BeadBug 6, Six Position Homogenizer, 115V
D1036 Benchmark Scientific 1 each 2617.7 EUR

BeadBug 6, Six Position Homogenizer, 230V
D1036-E Benchmark Scientific 1 PC 2617.7 EUR

BeadBug 6 Six Position Homogenizer 230V
HOM3018 Scientific Laboratory Supplies EACH 3400.8 EUR

BeadBlaster™ Microtube homogenizer, 115V
D2400 Benchmark Scientific 1 each 9275.1 EUR

BeadBlaster™ Microtube homogenizer, 230V
D2400-E Benchmark Scientific 1 PC 9275.1 EUR

BeadBlaster Microtube homogenizer 230V
HOM3012 Scientific Laboratory Supplies EACH 12158.4 EUR

BeadBug Microtube homogenizer
SLS1402 Scientific Laboratory Supplies EACH 1448.4 EUR

BeadBlaster™ 24 Refrigerated Microtube Homogenizer, 115V
D2400-R Benchmark Scientific 1 each 14802.1 EUR

BeadBlaster™ 24 Refrigerated Microtube Homogenizer, 230V
D2400-R-E Benchmark Scientific 1 each 14802.1 EUR

BeadBlaster 24 Refrigerated Microtube Homogenizer 230V
HOM3078 Scientific Laboratory Supplies EACH 22038 EUR

Homogenizer stand for Agile? Hand-held homogenizer
AHM1-VS ACTGene each 634.8 EUR

Homogenizer stand for Agile™ Hand-held homogenizer
AHM1 ACTGene VS 414.21 EUR

Microtube homogenizer, 115V
BCM1200 Bio Basic 1 pcs, 1 UNIT 11944.61 EUR

Microtube homogenizer, 115V
BCM1201 Bio Basic 1 pcs, 1 UNIT 1224.14 EUR

Dounce Tissue Homogenizer
1998-1 Biovision each 470.4 EUR

Pulse 150 Ultrasonic Homogenizer
HOM3082 Scientific Laboratory Supplies EACH 3891.6 EUR

D1000 Homogenizer incl 5mm and 7mm generators 230V
HOM3064 Scientific Laboratory Supplies EACH 1689.6 EUR

Pulse 150™ Ultrasonic Homogenizer with 6mm horn and soundproof box, 120V
DP0150 Benchmark Scientific 1 each 3428.4 EUR

Scalable and Robust Bacterial Cellulose Carbon Aerogels as Reusable Absorbents for High-Efficiency Oil/Water Separation

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Efficient selective separation of oils or organic pollutants from water is important for ecological, environmental conservation and sustainable development. Various absorption methods have emerged; the majority of them still suffer from defects including low removal efficiency, a complicated preparation process, and high cost. Herein, we present a highly porous and mechanical resilient bacterial cellulose (BC) carbon aerogel directly from BC hydrogel via facile directional freeze-drying and high-temperature carbonization. The resultant BC carbon aerogel showed excellent mechanical compressibility (maximal height compression ∼99.5%) and elastic recovery due to the porous structure. Taking advantages of the high thermal stability and superhydrophobicity, the BC carbon aerogel was directly used as a versatile adsorbent for oil/water separation.
The result demonstrated that the BC carbon aerogel showed super oil/water separation selectivity with the oil absorption capacity as high as 132-274 g g-1. More importantly, the BC carbon aerogel adsorbent can be reused by a simple absorption/combustion method and still keep high-efficiency oil absorption capacity and excellent superhydrophobicity after 20 absorption/combustion cycles, displaying recyclability and robust stability. In sum, the BC carbon aerogel introduced here is easy to fabricate, ecofriendly, highly scalable, low cost, mechanically robust, and reusable; https://biodas.org/ all of these features make it highly attractive for oil/water separation application.

A camphene-camphor-polymer composite material for the production of superhydrophobic absorbent microporous foams

In a recently published paper (doi.org/10.3390/molecules26113116) on self-propelled motion of objects on the water surface, we described a novel surface-active plastic material obtained by dissolution of camphor and polypropylene in camphene at 250 [Formula: see text]C. The material has wax-like mechanical properties, can be easily formed to any moldable shape, and allows for longer and more stable self-propelled motion if compared with pure camphor or pure camphene or of a camphene-camphor wax.
Here we use scanning electron microscopy to visualize and characterize the microporous structure of the solid polypropylene foam formed in the plastic for different polypropylene contents. The topology of foams remaining in the material after camphor and camphene molecules have been removed through evaporation or dissolution is similar to polypropylene foams obtained using thermally-induced phase separation. We show that the foams have a superhydrophobic surface but strongly absorb non-polar liquids, and suggest an array of potential scientific and industrial applications.

Development of pH-responsive absorbent pad based on polyvinyl alcohol/agarose/anthocyanins for meat packaging and freshness indication

Absorbent pads with antioxidant and pH-responsive color changing functions have been developed based on polyvinyl alcohol (PVA), agarose (AG), and purple sweet potato anthocyanins (PSPA), aiming for fresh keeping and freshness indication of fresh meat. The effects of PSPA content on the structure, physical properties, and colorimetric response towards pH changing of pads were evaluated. The results showed that PSPA interacted with PVA and AG and influenced the crystallinity, thermal stability and micro-morphology of pads.
The increase of the PSPA content from 3% to 12% improved the strength and DPPH radical scavenging activity of the pads, but reduced the swelling ratio. Significant color change of the pads was observed when pH increased from 3 to 10, and the pad containing 9% PSPA presented the most distinguishable color change with the change of pH. When applied as an absorbent pad for minced meat packaging, the pad indicated the real-time spoilage of the meat through obvious color change, and also extended the shelf life by at least 24 h. Therefore, the dual-functional pad shows great potential to be applied as a smart and active packaging for fresh meat, which would play an important role in ensuring food safety and improving food storage quality.

Occurrence and distribution of organic ultraviolet absorbents in sediments from small urban rivers, Tianjin, China: Implications for risk management

Organic ultraviolet absorbents (OUVAs) in the environment have been of increasing concern because of their potential hazards. However, the OUVAs in waters is far from being well studied and little is known about their occurrence in small urban rivers. This study investigated the concentrations and distribution of eleven OUVAs in the sediments from five small urban rivers of Tianjin, China, and found total concentrations in the range of 11.6-189 ng/g dry weight. Relative to other rivers and lakes, no high concentrations of sediment OUVAs were observed in the small rivers. Benzophenone, homosalate and octocrylene were the dominant OUVAs, representing medians of 13.3%, 12.4% and 12.3% of the total concentrations, respectively.
Our observed composition profiles of these chemicals were different from those found in most of other waters. The sediment OUVAs may originate more from industrial activities than the use of cosmetics and personal care products in this area. The risk to aquatic organisms from exposure to the sediment OUVAs in these small urban rivers was considered low, except for benzophenone. However, more researches are needed to investigate the pollution and associated risks of these chemicals in urban rivers due to the complexity of their toxicity to aquatic organisms.

Mixture Compound Fertilizer and Super Absorbent Polymer Application Significantly Promoted Growth and Increased Nutrient Levels in Pinus massoniana Seedlings and Soil in Seriously Eroded Degradation Region of Southern China

  • Pinus massoniana is the pioneer tree species in the red soil regions of southern China, however, the serious understory soil erosion and nutrient deficiency in that region are the main factors restricting the growth of P. massoniana. This field study examined the effects of compound fertilizer and super absorbent polymer (SAP) on the physiology, growth characteristics, biomass, soil nutrient, plant nutrient content, and nutrient uptake efficiency of 1-year-old P. massoniana seedlings for 2 years at Changting, Fujian in South China. One control (no fertilizer, CK) and fertilization treatments were established, namely, single compound fertilizer application (0.94, 1.89, and 3.56 g⋅plant-1) and mixture compound fertilizer and SAP application (0.94 + 1.01, 1.89 + 1.01, and 3.56 + 1.01 g⋅plant-1).
  • Fertilization significantly improved the physiological performance, root collar diameter growth, height growth, biomass, and nutrient uptake of the seedlings. Compared with other fertilization treatments, the mixture compound fertilizer and SAP application significantly improved the seedling photosynthesis, which meant that the SAP had a significant effect on promoting photosynthesis. Under the mixture compound fertilizer and SAP application, the whole biomass of the seedlings was higher than that of all other treatments. Fertilization significantly increased the nitrogen (N), phosphorus (P), and potassium (K) content in the soils, leaves, stems, and roots of the seedlings, respectively.
  • The P content was the main factor affecting growth characteristics and contributed to 58.03% of the total variation in seedling growth characteristics (P < 0.01). The N:P ratio of CK in the soils, leaves, and stems were higher than that of all the fertilization treatments, indicating that the severely eroded and degraded region had little P and required much of P. The principal component analysis indicated that the F2S (1.89 + 1.01 g) was the optimum fertilization amount and method in this experiment. These results provide a theoretical basis for the fertilization management of P. massoniana forests with severely eroded and degraded red soil regions.

Absorbent paper roll
DD30640 Scientific Laboratory Supplies EACH 25.2 EUR

Cotton Wool White Absorbent 500g
COT1000 Scientific Laboratory Supplies EACH 28.46 EUR

Human anti-Mouse Antibody Absorbent (HAMA)
HAMA Alpha Diagnostics 1 gram 343.2 EUR

Chemical Absorbent Pads 50cm x 40cm Yellow
SAF3730 Scientific Laboratory Supplies PK100 121.42 EUR

Maintenance Absorbent Roll 50cm x 40m Grey
SAF3750 Scientific Laboratory Supplies EACH 120.12 EUR

Absorbent Chemical Spill Response Kit 15L Clip-Top Bag
SAF3722 Scientific Laboratory Supplies EACH 54.05 EUR

Absorbent Chemical Spill Response Kit 20L Clip-Top Bag
SAF3724 Scientific Laboratory Supplies EACH 56.92 EUR

RF Absorbent for the removal of IgG in human plasma/serum
RF-ABS Alpha Diagnostics 100 tests 270 EUR

Absorbent Chemical Spill Response Kit 80L Circular Static Bin
SAF3716 Scientific Laboratory Supplies EACH 157.98 EUR

Cryo Express Dry Shipper with replaceable absorbent Material (CXR100)
TW-CXR100 MiTeGen 1 SHIPPER 1365 EUR

S. Pneumococcal CWPS/22F Absorbent solution for removing/adsorbing non-specific CWPS/22F from human or animal samples (sufficient for 50 samples)
560-CW-ABS Alpha Diagnostics 1 vial 416.4 EUR

100ml absorbency pad
SCIEA08 Scientific Laboratory Supplies PK500 117.42 EUR

A6 95kPa Specimen Transport Pouch with 20mL Absorbency Pad
COV1138 Scientific Laboratory Supplies PK1000 1279.49 EUR

A5 Specimen bag with Document Pouch Inserted 20ml absorbency
COV1147 Scientific Laboratory Supplies PK500 378.62 EUR

450ML Carbamate-1 CO2 Absorber
NAT1386 Scientific Laboratory Supplies 450ML 91.2 EUR

Goat anti-Mouse IgG(H+L) Cross absorbed against human immunoglobulins
E16SPU1011-100 EnoGene 100µg 286.8 EUR

Goat anti-Mouse IgG(H+L) Cross absorbed against human immunoglobulins
E16SPU1011-1000 EnoGene 1000µg 520.8 EUR

Goat anti-Mouse IgG(H+L) Cross absorbed against human immunoglobulins
E16SPU1011-500 EnoGene 500µg 411.6 EUR

Goat anti-Rabbit IgG(H+L) Cross absorbed against human immunoglobulins
E16SPU1021-100 EnoGene 100µg 286.8 EUR

Goat anti-Rabbit IgG(H+L) Cross absorbed against human immunoglobulins
E16SPU1021-1000 EnoGene 1000µg 520.8 EUR

Goat anti-Rabbit IgG(H+L) Cross absorbed against human immunoglobulins
E16SPU1021-500 EnoGene 500µg 411.6 EUR

Goat anti-Human IgG(H+L) Cross absorbed against mouse immunoglobulins
E16SPU1031-100 EnoGene 100µg 286.8 EUR

Superhydrophobic paper in the development of disposable labware and lab-on-paper devices

by
Traditionally in superhydrophobic surfaces history, the focus has frequently settled on the use of complex processing methodologies using nonbiodegradable and costly materials. In light of recent events on lab-on-paper emergence, there are now some efforts for the production of superhydrophobic paper but still with little development and confined to the fabrication of flat devices. This work gives a new look at the range of possible applications of bioinspired superhydrophobic paper-based substrates, obtained using a straightforward surface modification with poly(hydroxybutyrate). As an end-of-proof of the possibility to create lab-on-chip portable devices, the patterning of superhydrophobic paper with different wettable shapes is shown with low-cost approaches.
Furthermore, we suggest the use of superhydrophobic paper as an extremely low-cost material to design essential nonplanar lab apparatus, including reservoirs for liquid storage and manipulation, funnels, tips for pipettes, or accordion-shaped substrates for liquid transport or mixing. Such devices take the advantage of the self-cleaning and extremely water resistance properties of the surfaces https://biodas.org/ as well as the actions that may be done with paper such as cut, glue, write, fold, warp, or burn. The obtained substrates showed lower propensity to adsorb proteins than the original paper, kept superhydrophobic character upon ethylene oxide sterilization and are disposable, suggesting that the developing devices could be especially adequate for use in contact with biological and hazardous materials.

Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents

Zinc is an allosteric modulator of glycine receptor function, enhancing the effects of glycine at nM to low μM concentrations, and inhibiting its effects at higher concentrations. Because of zinc’s high potency at the glycine receptor, there exists a possibility that effects attributed solely to exogenously-applied glycine in fact contain an undetected contribution of zinc acting as an allosteric modulator. We found that glycine solutions made up in standard buffers and using deionized distilled water produced effects that could be decreased by the zinc chelator tricine.
This phenomenon was observed in three different vials tested and persisted even if vials were extensively washed, suggesting the zinc was probably present in the buffer constituents. In addition, polystyrene, but not glass, pipets bore a contaminant that enhanced glycine receptor function and that could also be antagonized by tricine. Our findings suggest that without checking for this effect using a chelator such as tricine, one cannot assume that responses elicited by glycine applied alone are not necessarily also partially due to some level of allosteric modulation by zinc.

Labware additives identified to be selective monoamine oxidase-B inhibitors

Plastic labware is used in all processes of modern pharmaceutical research, including compound storage and biological assays. The use of these plastics has created vast increases in productivity and cost savings as experiments moved from glass test tubes and capillary pipettes to plastic microplates and multichannel liquid handlers. One consequence of the use of plastic labware, however, is the potential release of contaminants and their resultant effects on biological assays.
We report herein the identification of biologically active substances released from a commonly used plastic microplate. The active contaminants were identified by gas chromatography-mass spectroscopy as dodecan-1-ol, dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate, and dodecanoic acid, and they were found to be selective monoamine oxidase-B inhibitors.

Open Labware: 3-D printing your own lab equipment

The introduction of affordable, consumer-oriented 3-D printers is a milestone in the current “maker movement,” which has been heralded as the next industrial revolution. Combined with free and open sharing of detailed design blueprints and accessible development tools, rapid prototypes of complex products can now be assembled in one’s own garage–a game-changer reminiscent of the early days of personal computing. At the same time, 3-D printing has also allowed the scientific and engineering community to build the “little things” that help a lab get up and running much faster and easier than ever before.

3D Printing in the Laboratory: Maximize Time and Funds with Customized and Open-Source Labware

3D printing, also known as additive manufacturing, is the computer-guided process of fabricating physical objects by depositing successive layers of material. It has transformed manufacturing across virtually every industry, bringing about incredible advances in research and medicine. The rapidly growing consumer market now includes convenient and affordable “desktop” 3D printers. These are being used in the laboratory to create custom 3D-printed equipment, and a growing community of designers are contributing open-source, cost-effective innovations that can be used by both professionals and enthusiasts.
User stories from investigators at the National Institutes of Health and the biomedical research community demonstrate the power of 3D printing to save valuable time and funding. While adoption of 3D printing has been slow in the biosciences to date, the potential is vast. The market predicts that within several years, 3D printers could be commonplace within the home; with so many practical uses for 3D printing, we anticipate that the technology will also play an increasingly important role in the laboratory.

3D-Printed Labware for High-Throughput Immobilization of Enzymes

  1. In continuous flow biocatalysis, chemical transformations can occur under milder, greener, more scalable, and safer conditions than conventional organic synthesis. However, the method typically involves extensive screening to optimize each enzyme’s immobilization on its solid support material. The task of weighing solids for large numbers of experiments poses a bottleneck for screening enzyme immobilization conditions.
  2. For example, screening conditions often require multiple replicates exploring different support chemistries, buffer compositions, and temperatures. Thus, we report 3D-printed labware designed to measure and handle solids in multichannel format and expedite screening of enzyme immobilization conditions.
  3. To demonstrate the generality of these advances, alkaline phosphatase, glucose dehydrogenase, and laccase were screened for immobilization efficiency on seven resins. The results illustrate the requirements for optimization of each enzyme’s loading and resin choice for optimal catalytic performance. Here, 3D-printed labware can decrease the requirements for an experimentalist’s time by >95%.
  4. The approach to rapid optimization of enzyme immobilization is applicable to any enzyme and many solid support resins. Furthermore, the reported devices deliver precise and accurate aliquots of essentially any granular solid material.

Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research

Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials’ walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable.
The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions’ adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.

Benchmark Agarose LE, 25g
A1700 Benchmark Scientific 1 PC 56.3 EUR

Benchmark Agarose LE, 100g
A1701 Benchmark Scientific 1 PC 135.8 EUR

Benchmark Agarose LE, 500g
A1705 Benchmark Scientific 1 PC 473.2 EUR

Benchmark Agarose 3:1, 100g
A1801-31 Benchmark Scientific 1 PC 287 EUR

Benchmark Agarose LM, Low Melt, 100g
A1801-LM Benchmark Scientific 1 PC 377.1 EUR

Benchmark Agarose HR, PCR Grade for DNA fragments between 20 to 800bp, 100g
A1801-HR Benchmark Scientific 1 PC 326.1 EUR

Benchmark Printer 230V
AUT2743 Scientific Laboratory Supplies EACH 488.29 EUR

Benchmark Digital Hotplate 230V
MIX1262 Scientific Laboratory Supplies EACH 416.4 EUR

Benchmark Orbi-Shaker CO2 230V
MIX7260 Scientific Laboratory Supplies EACH 5173.2 EUR

Benchmark Hotplate 17.8cm x 17.8cm 230V
MIX1301 Scientific Laboratory Supplies EACH 390 EUR

Benchmark Replacement Cap Blue 50mL
BOT1910 Scientific Laboratory Supplies PK10 46.8 EUR

Benchmark Refill Glass Beads 3mm 1000g
STE1042 Scientific Laboratory Supplies EACH 42.12 EUR

Benchmark Digital Magnetic Stirrer 230V
MIX1263 Scientific Laboratory Supplies EACH 416.4 EUR

Benchmark MiniMag Magnetic Stirrer 240V
MIX1290 Scientific Laboratory Supplies EACH 177.6 EUR

Benchmark MyFuge 5 MicroCentrifuge 230V
CEN1870 Scientific Laboratory Supplies EACH 700.8 EUR

Benchmark Replacement Sealing Ring 50mL
BOT1911 Scientific Laboratory Supplies PK10 20.4 EUR

Benchmark Magnetic Stirrer 17.8cm x 17.8cm 230V
MIX1302 Scientific Laboratory Supplies EACH 390 EUR

Benchmark StripSpin 12 Mini Centrifuge 230V
CEN1714 Scientific Laboratory Supplies EACH 507.6 EUR

Benchmark Replacement Sealing Ring 100-2000mL
BOT1909 Scientific Laboratory Supplies PK10 20.4 EUR

Benchmark StripSpin Round Rotor 8 position
CEN1715 Scientific Laboratory Supplies EACH 78 EUR

Benchmark 8 x 5mL Rotor for MC-24 Touch
CEN1850 Scientific Laboratory Supplies EACH 1441.2 EUR

Benchmark BenchMasher Blender Bag 400mL x 60um 300 x 180mm
HOM3102 Scientific Laboratory Supplies PK500 118.8 EUR

Benchmark hybex Replacement Cap Blue 100-2000mL
BOT1867 Scientific Laboratory Supplies PK10 52.62 EUR

Benchmark High Temperature Cap Red (up to 180C)
BOT1908 Scientific Laboratory Supplies PK10 199.2 EUR

Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents

by
Zinc is an allosteric modulator of glycine receptor function, enhancing the effects of glycine at nM to low μM concentrations, and inhibiting its effects at higher concentrations. Because of zinc’s high potency at the glycine receptor, there exists a possibility that effects attributed solely to exogenously-applied glycine in fact contain an undetected contribution of zinc acting as an allosteric modulator. We found that glycine solutions made up in standard buffers and using deionized distilled water produced effects that could be decreased by the zinc chelator tricine.
This phenomenon was observed in three different vials tested and persisted even if vials were extensively washed, suggesting the zinc was probably present in the buffer constituents. In addition, polystyrene, but not glass, pipets bore a contaminant that enhanced glycine receptor function and that could also be antagonized by tricine. Our findings suggest that without checking for this effect using a chelator such as tricine, one cannot assume that responses elicited by glycine applied alone are not necessarily also partially due to some level of allosteric modulation by zinc.

Superhydrophobic paper in the development of disposable labware and lab-on-paper devices

Traditionally in superhydrophobic surfaces history, the focus has frequently settled on the use of complex processing methodologies using nonbiodegradable and costly materials. In light of recent events on lab-on-paper emergence, there are now some efforts for the production of superhydrophobic paper https://biodas.org/ but still with little development and confined to the fabrication of flat devices. This work gives a new look at the range of possible applications of bioinspired superhydrophobic paper-based substrates, obtained using a straightforward surface modification with poly(hydroxybutyrate). As an end-of-proof of the possibility to create lab-on-chip portable devices, the patterning of superhydrophobic paper with different wettable shapes is shown with low-cost approaches.
Furthermore, we suggest the use of superhydrophobic paper as an extremely low-cost material to design essential nonplanar lab apparatus, including reservoirs for liquid storage and manipulation, funnels, tips for pipettes, or accordion-shaped substrates for liquid transport or mixing. Such devices take the advantage of the self-cleaning and extremely water resistance properties of the surfaces as well as the actions that may be done with paper such as cut, glue, write, fold, warp, or burn. The obtained substrates showed lower propensity to adsorb proteins than the original paper, kept superhydrophobic character upon ethylene oxide sterilization and are disposable, suggesting that the developing devices could be especially adequate for use in contact with biological and hazardous materials.

3D Printing in the Laboratory: Maximize Time and Funds with Customized and Open-Source Labware

3D printing, also known as additive manufacturing, is the computer-guided process of fabricating physical objects by depositing successive layers of material. It has transformed manufacturing across virtually every industry, bringing about incredible advances in research and medicine. The rapidly growing consumer market now includes convenient and affordable “desktop” 3D printers. These are being used in the laboratory to create custom 3D-printed equipment, and a growing community of designers are contributing open-source, cost-effective innovations that can be used by both professionals and enthusiasts.
User stories from investigators at the National Institutes of Health and the biomedical research community demonstrate the power of 3D printing to save valuable time and funding. While adoption of 3D printing has been slow in the biosciences to date, the potential is vast. The market predicts that within several years, 3D printers could be commonplace within the home; with so many practical uses for 3D printing, we anticipate that the technology will also play an increasingly important role in the laboratory.

3D-Printed Labware for High-Throughput Immobilization of Enzymes

In continuous flow biocatalysis, chemical transformations can occur under milder, greener, more scalable, and safer conditions than conventional organic synthesis. However, the method typically involves extensive screening to optimize each enzyme’s immobilization on its solid support material. The task of weighing solids for large numbers of experiments poses a bottleneck for screening enzyme immobilization conditions. For example, screening conditions often require multiple replicates exploring different support chemistries, buffer compositions, and temperatures.
Thus, we report 3D-printed labware designed to measure and handle solids in multichannel format and expedite screening of enzyme immobilization conditions. To demonstrate the generality of these advances, alkaline phosphatase, glucose dehydrogenase, and laccase were screened for immobilization efficiency on seven resins. The results illustrate the requirements for optimization of each enzyme’s loading and resin choice for optimal catalytic performance. Here, 3D-printed labware can decrease the requirements for an experimentalist’s time by >95%. The approach to rapid optimization of enzyme immobilization is applicable to any enzyme and many solid support resins. Furthermore, the reported devices deliver precise and accurate aliquots of essentially any granular solid material.

Additive manufactured customizable labware for biotechnological purposes

Yet already developed in the 1980s, the rise of 3D printing technology did not start until the beginning of this millennium as important patents expired, which opened the technology to a whole new group of potential users. One of the first who used this manufacturing tool in biotechnology was Lücking et al. in 2012, demonstrating potential uses 1, 2. This study shows applications of custom-built 3D-printed parts for biotechnological experiments. It gives an overview about the objects’ computer-aided design (CAD) followed by its manufacturing process and basic studies on the used printing material in terms of biocompatibility and manageability. Using the stereolithographic (SLA) 3D-printing technology, a customizable shake flask lid was developed, which was successfully used to perform a bacterial fed-batch shake flask cultivation.
The lid provides Luer connectors and tube adapters, allowing both sampling and feeding without interrupting the process. In addition, the digital blueprint the lid is based on, is designed for a modular use and can be modified to fit specific needs. All connectors can be changed and substituted in this CAD software-based file. Hence, the lid can be used for other applications, as well. The used printing material was tested for biocompatibility and showed no toxic effects neither on mammalian, nor on bacteria cells. Furthermore an SDS-PAGE-comb was drawn and printed and its usability evaluated to demonstrate the usefulness of 3D printing for everyday labware. The used manufacturing technique for the comb (multi jet printing, MJP) generates highly smooth surfaces, allowing this application.

Benchmark Agarose LE, 25g
A1700 Benchmark Scientific 1 PC 56.3 EUR

Benchmark Agarose LE, 100g
A1701 Benchmark Scientific 1 PC 135.8 EUR

Benchmark Agarose LE, 500g
A1705 Benchmark Scientific 1 PC 473.2 EUR

Benchmark Agarose 3:1, 100g
A1801-31 Benchmark Scientific 1 PC 287 EUR

Benchmark Agarose LM, Low Melt, 100g
A1801-LM Benchmark Scientific 1 PC 377.1 EUR

Benchmark Agarose HR, PCR Grade for DNA fragments between 20 to 800bp, 100g
A1801-HR Benchmark Scientific 1 PC 326.1 EUR

Benchmark Printer 230V
AUT2743 Scientific Laboratory Supplies EACH 488.29 EUR

Benchmark Digital Hotplate 230V
MIX1262 Scientific Laboratory Supplies EACH 416.4 EUR

Benchmark Orbi-Shaker CO2 230V
MIX7260 Scientific Laboratory Supplies EACH 5173.2 EUR

Benchmark Hotplate 17.8cm x 17.8cm 230V
MIX1301 Scientific Laboratory Supplies EACH 390 EUR

Benchmark Replacement Cap Blue 50mL
BOT1910 Scientific Laboratory Supplies PK10 46.8 EUR

Benchmark Refill Glass Beads 3mm 1000g
STE1042 Scientific Laboratory Supplies EACH 42.12 EUR

Benchmark Digital Magnetic Stirrer 230V
MIX1263 Scientific Laboratory Supplies EACH 416.4 EUR

Benchmark MiniMag Magnetic Stirrer 240V
MIX1290 Scientific Laboratory Supplies EACH 177.6 EUR

Benchmark MyFuge 5 MicroCentrifuge 230V
CEN1870 Scientific Laboratory Supplies EACH 700.8 EUR

Benchmark Replacement Sealing Ring 50mL
BOT1911 Scientific Laboratory Supplies PK10 20.4 EUR

Benchmark Magnetic Stirrer 17.8cm x 17.8cm 230V
MIX1302 Scientific Laboratory Supplies EACH 390 EUR

Benchmark StripSpin 12 Mini Centrifuge 230V
CEN1714 Scientific Laboratory Supplies EACH 507.6 EUR

Benchmark Replacement Sealing Ring 100-2000mL
BOT1909 Scientific Laboratory Supplies PK10 20.4 EUR

Benchmark StripSpin Round Rotor 8 position
CEN1715 Scientific Laboratory Supplies EACH 78 EUR

Benchmark 8 x 5mL Rotor for MC-24 Touch
CEN1850 Scientific Laboratory Supplies EACH 1441.2 EUR

Benchmark BenchMasher Blender Bag 400mL x 60um 300 x 180mm
HOM3102 Scientific Laboratory Supplies PK500 118.8 EUR

Benchmark hybex Replacement Cap Blue 100-2000mL
BOT1867 Scientific Laboratory Supplies PK10 52.62 EUR

Benchmark High Temperature Cap Red (up to 180C)
BOT1908 Scientific Laboratory Supplies PK10 199.2 EUR

An Economical, Portable Manual Cryogenic Plunge Freezer for the Preparation of Vitrified Biological Samples for Cryogenic Electron Microscopy.

by

Visualizing biological structures and cellular processes in their native state is a major goal of many scientific laboratories. In the past 20 years, the technique of preserving samples by vitrification has greatly expanded, specifically for use in cryogenic electron microscopy (cryo-EM). Here, we report on improvements in the design and use of a portable manual cryogenic plunge freezer that is intended for use in laboratories that are not equipped for the cryopreservation of samples.

The construction of the instrument is economical, can be produced by a local machine shop without specialized equipment, and lowers the entry barriers for newcomers with a reliable alternative to costly commercial equipment. The improved design allows for successful freezing of isolated proteins for single particle analysis https://biodas.org/ as well as bacterial cells for cryo-electron tomography. With this instrument, groups will be able to prepare vitreous samples whenever and wherever necessary, which can then be imaged at local or national cryo-EM facilities.

Successful short-term cryopreservation of volume-reduced cord blood units in a cryogenic mechanical freezer: effects on cell recovery, viability, and clonogenic potential

BACKGROUND
Cord blood (CB) units are stored from weeks to years in liquid- or vapor-phase nitrogen until they are used for transplantation. We examined the effects of cryostorage in a mechanical freezer at -150°C on critical quality control variables of CB collections to investigate the possible use of mechanical freezers at -150°C as an alternative to storage in liquid- (or vapor-) phase nitrogen.
METHODS
A total of 105 CB units were thawed and washed at different time intervals (6, 12, 24, and 36 months). For every thawed CB unit, samples were removed and cell enumeration (total nucleated cells [TNCs], mononuclear cells [MNCs], CD34+, CD133+) was performed. In addition, viability was obtained with the use of flow cytometry, and recoveries were calculated. Also, total absolute colony-forming unit counts were performed and progenitor cell recoveries were studied by clonogenic assays.
RESULTS
Significant differences (p < 0.05) were observed in certain variables (TNCs, MNC numbers, viability) when they were examined in relation with time intervals, while others (CD34+, CD133+) were relatively insensitive (p = NS) to the duration of time interval the CB units were kept in cryostorage condition.
CONCLUSIONS
The data presented suggest that cryopreservation of CB units in a mechanical freezer at -150°C may represent an alternative cryostorage condition for CB cryopreservation.

Realignment-free cryogenic macroscopic optical cavity coupled to an optical fiber

We present a cryogenic setup where an optical Fabry-Perot resonator is coupled to a single-mode optical fiber with coupling efficiency above 90% at mK temperatures without realignment during cooling down. The setup is prealigned at room temperature to compensate for the thermal contraction and change of the refractive index of the optical components during cooling down.

The high coupling efficiency is achieved by keeping the setup rotation-symmetric around the optical axis. The majority of the setup components are made of Invar (FeNi36), which minimizes the thermal contraction. High coupling efficiency is essential in quantum optomechanical experiments.

Extraordinary approach to further boost plasmonic NIR-SERS by cryogenic temperature-suppressed non-radiative recombination

We report an effective strategy to promote the near-infrared surface-enhanced Raman scattering spectroscopy (NIR-SERS) activity by boosting the photon-induced charge transfer (PICT) efficiency at cryogenic temperature. Based on as-prepared Au/Ag nano-urchins (NUs) with abundant surface defects, the extremely low temperature (77 K) can significantly weaken the metallic lattice vibration and reduce the recombination of thermal phonons and photoexcited electrons, then accelerate the migration of energetic electrons.
It enables the NIR-SERS detection limit of dye molecules to be achieved at 10-17 M, which is nearly three orders of magnitude better than that at room temperature. The present work provides a new, to the best of our knowledge, approach for ultra-trace NIR-SERS bioanalysis.

Ultra-stretchable and fast self-healing ionic hydrogel in cryogenic environments for artificial nerve fiber

Self-healing materials behave irreplaceable advantages in biomimetic intelligent robots (BIR) for avoiding or reducing safety hazards and economic losses from accidental damage during service. However, the self-healing ability is unreservedly lost and even becomes rigid, fragile in the cryogenic environment where BIR is precisely needed. Here, we report a versatile ionic hydrogel with fast self-healing ability, ultra-stretchability, and stable conductivity, even at -80℃.
The hydrogel is systematically optimized to improve hydrogen-bonded network nanostructure, coordinated achieving a quick self-healing ability within 10 min, large deformation tolerance of over 7000%, superior conductivity of 11.76 S·cm-1 and anti-freezing ability, which is difficult to obtain simultaneously. Such hydrogel provides new opportunities for artificial electronic devices in harsh environments. As a prospective application, we fabricate an artificial nerve fiber by mimicking the structure and functions of the myelinated axon, exhibiting the property of fast and potential-gated signal transmission.
This artificial nerve fiber is integrated into a robot for demonstrating a real-time high fidelity and high throughput information interaction under big deformation and cryogenic temperature. The hydrogel and bionic device will bring pioneering functions for robots and open a broad application scenario in extreme conditions. This article is protected by copyright. All rights reserved.

Cryogenic temperature sensing based on the temperature dependence of color centers in optical fibers

A cryogenic temperature sensor based on the temperature dependence of stable color centers in a commercial single-mode optical fiber is proposed. The radiation induced attenuation spectra at different temperatures are measured and decomposed by Ge-NBOHC and Ge(X) color centers. The configurational coordinate model is used to explain the temperature properties of the color centers.
A series of experiments are conducted to evaluate its performance in the temperature range from 10°C to -196°C, and the results suggest that the temperature sensitivity is ∼0.17 dB/km/°C with a resolution of 0.034°C, and the nonlinearity and repeatability error are ±3.8% and 1.4%, respectively.

Revealing the Intrinsic Atomic Structure and Chemistry of Amorphous LiO 2-Containing Products in Li-O 2 Batteries Using Cryogenic Electron Microscopy

Aprotic lithium-oxygen batteries (LOBs) are promising energy storage systems characterized by ultrahigh theoretical energy density. Extensive research has been devoted to this battery technology, yet the detailed operational mechanisms involved, particularly unambiguous identification of various discharge products and their specific distributions, are still unknown or are subjects of controversy. This is partly because of the intrinsic complexity of the battery chemistry but also because of the lack of atomic-level insight into the oxygen electrodes acquired via reliable techniques. In the current study, it is demonstrated that electron beam irradiation could induce crystallization of amorphous discharge products. Cryogenic conditions and a low beam dosage have to be used for reliable transmission electron microscopy (TEM) characterization.
High-resolution cryo-TEM and electron energy loss spectroscopy (EELS) analysis of toroidal discharge particles unambiguously identified the discharge products as a dominating amorphous LiO2 phase with only a small amount of nanocrystalline Li2O2 islands dispersed in it. In addition, uniform mixing of carbon-containing byproducts is identified in the discharge particles with cryo-EELS, which leads to a slightly higher charging potential. The discharge products can be reversibly cycled, with no visible residue after full recharge. We believe that the amorphous superoxide dominating discharge particles can lead researchers to reconsider the chemistry of LOBs and pay special attention to exclude beam-induced artifacts in traditional TEM characterizations.

10K Cryogenic Freezer With CS200 Controller and Gas ByPass
TW-10K-CS200-GBP MiTeGen 1 UNIT 19442 EUR

24K Cryogenic Freezer With CS200 Controller and Gas ByPass
TW-24K-CS200-GBP MiTeGen 1 UNIT 22879 EUR

38K Cryogenic Freezer With CS200 Controller and Gas ByPass
TW-38K-CS200-GBP MiTeGen 1 UNIT 31556 EUR

80K Cryogenic Freezer With CS200 Controller and Double Step
TW-LABS80K-CS-DS MiTeGen 1 UNIT 64636 EUR

80K Cryogenic Freezer With CS200 Controller and Locking Step
TW-LABS80K-CS MiTeGen 1 UNIT 63686 EUR

10K Cryogenic Freezer No Controller
TW-10K MiTeGen 1 UNIT 14003 EUR

24K Cryogenic Freezer No Controller
TW-24K MiTeGen 1 UNIT 19318 EUR

38K Cryogenic Freezer No Controller
TW-38K MiTeGen 1 UNIT 28465 EUR

94K Cryogenic Freezer With CS200 Controller and Double Locking Steps and Fill Hose with Adapter
TW-LABS94K-SP MiTeGen 1 UNIT 71752 EUR

10K Cryogenic Freezer With CS100 Controller
TW-10K-CS100 MiTeGen 1 UNIT 17922 EUR

10K Cryogenic Freezer With CS200 Controller
TW-10K-CS200 MiTeGen 1 UNIT 18872 EUR

24K Cryogenic Freezer With CS100 Controller
TW-24K-CS100 MiTeGen 1 UNIT 21359 EUR

24K Cryogenic Freezer With CS200 Controller
TW-24K-CS200 MiTeGen 1 UNIT 22309 EUR

38K Cryogenic Freezer With CS200 Controller
TW-38K-CS200 MiTeGen 1 UNIT 30606 EUR

20K Cryogenic Freezer With CS200 Controller
TW-LABS20K-CS MiTeGen 1 UNIT 29839 EUR

40K Cryogenic Freezer With CS200 Controller
TW-LABS40K-CS MiTeGen 1 UNIT 41579 EUR

3K Cryogenic Freezer With Stainless Steel Exterior
TW-3KSBL MiTeGen 1 UNIT 3870 EUR

Special cryogenics label 38 x 19 mm white colour
DD53075 Scientific Laboratory Supplies PK1200 160.8 EUR

Special cryogenics label 33 x 13 mm assorted colours
DD53520 Scientific Laboratory Supplies PK1700 160.8 EUR

Special cryogenics label 38 x 6 mm assorted colours
DD53526 Scientific Laboratory Supplies PK3120 154.8 EUR

CO2 Back Up for ULT Freezers
SLS1072 Scientific Laboratory Supplies EACH 3034.8 EUR

COOLCELL® LX-4 PACK, 4 COLOURS, CELL FREEZING CONTAINER, FOR 12 X 1ML OR 2ML CRYOGENIC VIALS
432138 CORNING 1/pk 714 EUR

NBS CO2 Backup for Innova Freezers
FRE6110 Scientific Laboratory Supplies EACH 2235.6 EUR

NBS LN2 Backup for Innova Freezers
FRE6114 Scientific Laboratory Supplies EACH 2235.6 EUR

NBS CO2 Backup for Premium Freezers
FRE6112 Scientific Laboratory Supplies EACH 2077.2 EUR

NBS LN2 Backup for Premium Freezers
FRE6116 Scientific Laboratory Supplies EACH 2235.6 EUR

Mutations in the receptor-binding domain of human SARS CoV-2 spike protein increases its affinity to bind human ACE-2 receptor

by
The severe acute respiratory syndrome virus-2 (SARS CoV-2) infection has resulted in the current global pandemic. The binding of SARS CoV-2 spike protein receptor-binding domain (RBD) to the human angiotensin converting enzyme-2 (ACE-2) receptor causes the host infection. The spike protein has undergone several mutations with reference to the initial strain isolated during December 2019 from Wuhan, China. A number of these mutant strains have been reported as variants of concern and as variants being monitored. Some of these mutants are known to be responsible for increased transmissibility of the virus.
The reason for the increased transmissibility caused by the point mutations can be understood by studying the structural implications and inter-molecular interactions in the binding of viral spike protein RBD and human ACE-2. Here, we use the crystal structure of the RBD in complex with ACE-2 available in www.joplink.net/coronavirus-proteins/ the public domain and analyse the 250 ns molecular dynamics (MD) simulations of wild-type and mutants; K417N, K417T, N440K, N501Y, L452R, T478K, E484K and S494P.
The ionic, hydrophobic and hydrogen bond interactions, amino acid residue flexibility, binding energies and structural variations are characterized. The MD simulations provide clues to the molecular mechanisms of ACE-2 receptor binding in wild-type and mutant complexes. The mutant spike proteins RBD were associated with greater binding affinity with ACE-2 receptor. Communicated by Ramaswamy H. Sarma.

COVID-19 and Alzheimer’s disease: Meninges-mediated neuropathology

SARS-CoV-2 the causative agent of COVID-19 displays a broad range of pathophysiology. Cytokine storms associated with COVID-19 damage the blood-brain barrier (BBB) and allow pro-inflammatory factors to invade the brain, further promoting neurodegeneration. While SARS-CoV-2 viral RNA and proteins have been detected in brain tissues, the mechanisms of neuroinvasion remain unknown. COVID-19 has had a disproportionate impact on those suffering from neurodegenerative disorders such as Alzheimer’s disease (AD).
Understanding the mechanisms of SARS-CoV-2 neuroinvasion is crucial to study the long-term neurocognitive effects of COVID-19 on AD pathology.
Viruses can infiltrate the brain through the meninges via infected immune cells. The meninges regulate the immune surveillance of the brain and play a key role in the efflux of pathogens from the brain. Meningeal dysfunction has been demonstrated to exacerbate amyloid-beta pathogenesis. In this study, we explore the neuroinvasion pathway of SARS-CoV-2 through the meninges and its effect on AD pathology.
Method: 5x FAD x hACE2 mice were inoculated intranasally with a sublethal dose of SARS-CoV-2. The mice were maintained for 2 weeks. Mouse brains and meninges were harvested. The tissue was stained and immunofluorescence imaging was conducted to study viral proliferation and immune responses. Histo-cytometry was conducted for quantitative imaging analysis. Gene expression studies were done using Nanostring assays. All experiments involving the SARS-Cov-2 virus were carried out in a BSL3 facility.
Result: This ongoing study demonstrates the proliferation of the SARS-CoV-2 virus in the brain via meningeal lymphatics. SARS-CoV-2 infection resulted in increased neuroinflammation. Additionally, inflammatory responses induced meningeal dysfunction resulting in increased amyloid-beta pathology and cerebrospinal fluid drainage.
Conclusion: Given the increasing evidence for a viral hypothesis of Alzheimer’s Disease it is extremely important to study the neurodegenerative effects of COVID-19 which has affected millions worldwide. We demonstrate that SARS-CoV-2 infiltrates the brain via the meninges promoting neuroinflammation. Furthermore, amyloid-beta pathologies are exacerbated by COVID-19 in animal models providing preclinical evidence of the long-term neurodegenerative effects of COVID-19.

Exosomes Recovered From the Plasma of COVID-19 Patients Expose SARS-CoV-2 Spike-Derived Fragments and Contribute to the Adaptive Immune Response

Coronavirus disease 2019 (COVID-19) is an infectious disease caused by beta-coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that has rapidly spread across the globe starting from February 2020. It is well established that during viral infection, extracellular vesicles become delivery/presenting vectors of viral material. However, studies regarding extracellular vesicle function in COVID-19 pathology are still scanty. Here, we performed a comparative study on exosomes recovered from the plasma of either MILD or SEVERE COVID-19 patients.
We show that although both types of vesicles efficiently display SARS-CoV-2 spike-derived peptides and carry immunomodulatory molecules, only those of MILD patients are capable of efficiently regulating antigen-specific CD4+ T-cell responses. Accordingly, by mass spectrometry, we show that the proteome of exosomes of MILD patients correlates with a proper functioning of the immune system, while that of SEVERE patients is associated with increased and chronic inflammation.
Overall, we show that exosomes recovered from the plasma of COVID-19 patients possess SARS-CoV-2-derived protein material, have an active role in enhancing the immune response, and possess a cargo that reflects the pathological state of patients in the acute phase of the disease.

Role of SARS-CoV-2 in causing blood-brain barrier leakage and microglial activation as a risk factor of cognitive deterioration in subjects at risk of Alzheimer’s disease

The recent pandemic provides evidence of altered central nervous system (CNS) function in response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-COV-2 invades the CNS by binding angiotensin-converting enzyme 2 (ACE2) expressed on neurons and glia. SARS-COV-2 may have effects on increased permeability of endothelial cells within the blood-brain barrier (BBB) as studies have shown that the S1 protein can transverse the BBB.
This is interesting because leakage of the BBB is implicated in Alzheimer’s disease (AD) pathogenesis. We hypothesized that SARS-CoV-2 infection leads to innate stimulated inflammation, ultimately activating microglial cells and an influx of pro-inflammatory cytokines and leukocytes in the meninges, contributing to increased permeability of the BBB. This BBB permeability increases AD susceptibility in subjects at risk by causing irreversible damage to the BBB and microglial cell activation.
Method: We developed a double transgenic mouse model using mice expressing human ACE2 receptor and 5xFAD mice that exhibit increased neuropathology seen in human AD allowing modeling of AD in SARS-CoV-2 pathogenesis. The hACE2/5xFAD double transgenic mice were intranasally inoculated with a sub-lethal dose of SARS-CoV-2 to test the hypothesis that SARS-COV-2 potentiates AD pathology and cognitive deterioration through impairment of the BBB. Leukocyte and cytokine populations were measured by flow cytometry and single-nuclei RNA sequencing of the meninges for characterization of microglial populations.
Result: SARS-CoV-2 creates a cytotoxic environment in the brain immediately following infection in hACE2/5xFAD mice leading to leakage of the BBB in the meninges. Activation of microglial innate cells by SARS-COV-2 invasion of the CNS will cause neural deterioration having long term implications on cognitive function. The hACE2/5xFAD mouse model allows us to uncover implications for SARS-COV-2 on AD cognitive deterioration.
Conclusion: The hACE2/5xFAD mouse allows modeling of SARS-CoV-2 in developing AD cases and allowed us to determine the immune environment generated in the meninges in response to SARS-CoV-2 infections. This mouse model provides a platform to proactively determine the effects of SARS-CoV-2 in developing AD cases, a methodology to be exploited for future mouse models determining the relationship of other viruses on AD pathology, and the opportunity to address phenotypes with therapeutics for preventative initiatives.

Recombinant SARS SARS MERS Protein, His, E.coli-1mg
1mg 1513.2 EUR

Recombinant SARS SARS-CoV Protein, His, E.coli-1mg
1mg 4744.8 EUR

Recombinant SARS SARS-CoV Protein, His, E.coli-5ug
5ug 186 EUR

Recombinant SARS SARS MERS Protein, His, E.coli-100ug
100ug 261.6 EUR

Recombinant SARS SARS MERS Protein, His, E.coli-500ug
500ug 795.6 EUR

Recombinant SARS SARS-CoV Protein, His, E.coli-20ug
20ug 241.2 EUR

Recombinant SARS SARS Spike S1 Protein, Avi His Tag
20ug 495 EUR

Recombinant SARS SARS Spike RBD Protein, Avi His Tag
20ug 495 EUR

Recombinant SARS SARS Core Protein, Untagged, E.coli-1mg
1mg 1273.2 EUR

Recombinant SARS SARS Core Protein, Untagged, E.coli-1mg
1mg 1273.2 EUR

Perspectives of farmers and tourists on agricultural abandonment in east Lesvos, Greece.

by
Perspectives of farmers and tourists on agricultural abandonment in east Lesvos, Greece.

Multi-stakeholder perceptions of panorama modifications are more and more acknowledged as important inputs to discussions on future panorama developments, significantly when addressing the way forward for European rural areas experiencing agricultural abandonment.

This analysis presents a case exploration of abandonment of olive plantations in east Lesvos, Greece. We carried out two units of semi-structured interviews to narrate an exploration on native farmers’ capacity and willingness to keep up the plantations, to the outcomes of a panorama choice survey undertaken with vacationers. Three farmer varieties are recognized following a cluster evaluation based mostly on attributes of particular person capacity and willingness to farm. Farmers belonging to the prevalent kind revealed low capacity and willingness and count on to additional extensify their farms.

The remaining two farmer varieties have greater willingness; they’re motivated by cultural causes, extra incessantly expressing a want to keep up their land underneath household possession, and partake in social cooperative initiatives selling practices valorizing the olive plantations. We define how these varieties work together with regional drivers of change, and partly additionally contribute to persistence of abandonment by way of constrained capacity to farm.

Abandonment doesn’t align with present panorama preferences of vacationers, who favor cultivated landscapes, components of traditionality inside constructed infrastructure and undertake nature-based actions. We focus on how excessive willingness to farm related to skilled and pluri-active types of farming could nevertheless present alternatives to keep up the cultivated panorama and synergize with (agri-)tourism demand. Our findings are corresponding to these of different European research, contributing to discussions on the way forward for its rural landscapes.

Perspectives of farmers and tourists on agricultural abandonment in east Lesvos, Greece.
Views of farmers and vacationers on agricultural abandonment in east Lesvos, Greece.

Immunophenotypic characterization, multi-lineage differentiation and growing older of zebrafish coronary heart and liver tissue-derived mesenchymal stem cells as a novel strategy in stem cell-based remedy.

Mesenchymal stem cells (MSCs) are a very good mannequin for preclinical and scientific investigations, and various sources of MSCs are topic to intensive experiments. On this examine, mesenchymal stem cells (MSCs) have been remoted from coronary heart and liver tissue of Zebrafish (Danio rerio). The flow-cytometry in addition to RT-PCR have been used to investigate the expression of a panel of cell floor markers CD44, CD90, CD31 and CD34.

Rapid antibody fluorescent labeling kit (eFluor 405)
EGQ0042 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 488)
EGQ0043 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 555)
EGQ0044 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 594)
EGQ0045 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 647)
EGQ0046 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 680)
EGQ0047 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 750)
EGQ0048 1 labeing(100ug/T)
EUR 225

Paritaprevir(ABT-450)
20-abx184297
  • EUR 1496.40
  • EUR 1095.60
  • 10 mg
  • 5 mg

iFluor® 450 maleimide
1057 1 mg
EUR 222

AKT1 (Ab-450) Antibody
21502-100ul 100ul
EUR 302.4

AKT1 (Ab-450) Antibody
21502-50ul 50ul
EUR 224.4

AKT1 (Ab-450) Antibody
CSB-PA969941- each
EUR 402
Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

AKT1 (Ab-450) Antibody
CSB-PA969941-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

anti-AKT1 (Ab-450)
LF-PA20679 100 ul
EUR 400.8
Description: Rabbit polyclonal to AKT1

Nhe I unit: 450
YRNHE1 1 vial Ask for price

mFluorâ„¢ Violet 450 SE
1150-1mg 1 mg
EUR 222
Description: AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.

mFluorâ„¢ Violet 450 acid
1140-5mg 5 mg
EUR 222
Description: AAT Bioquest's mFluorâ„¢ dyes are developed for multicolor flow cytometry-focused applications.

mFluorâ„¢ Violet 450 maleimide
1600-1mg 1 mg
EUR 222
Description: AAT Bioquest\'s mFluorâ„¢ dyes are developed for multicolor flow cytometry-focused applications.

mFluorâ„¢ Violet 450 Azide
1690-1mg 1 mg
EUR 308
Description: AAT Bioquest's mFluorâ„¢ dyes are developed for multicolor flow cytometry-focused applications.

iFluor® 450 maleimide
1057-1mg 1 mg
EUR 222
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

Akt (Ab-450) Antibody
E11-0406B 100μg
EUR 225
Description: Available in various conjugation types.

AKT1 (Ab-450) Antibody
E021502-1 50μg/50μl
EUR 100
Description: Available in various conjugation types.

AKT1 (Ab-450) Antibody
E021502-2 100μg/100μl
EUR 170
Description: Available in various conjugation types.

Polyclonal SNX4 Antibody (aa438-450)
AMM07924G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human SNX4 (aa438-450). This antibody is tested and proven to work in the following applications:

Polyclonal PACSIN2 Antibody (aa400-450)
APR17733G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PACSIN2 (aa400-450). This antibody is tested and proven to work in the following applications:

Polyclonal CYP1B1 Antibody (aa400-450)
APG02857G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CYP1B1 (aa400-450). This antibody is tested and proven to work in the following applications:

Polyclonal TLR1 Antibody (aa400-450)
APR02824G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TLR1 (aa400-450). This antibody is tested and proven to work in the following applications:

mFluor™ Violet 450 maleimide
1600 1 mg
EUR 222

mFluor™ Violet 450 Azide
1690 1 mg
EUR 308

mFluor™ Violet 450 SE
1150 1 mg
EUR 222

mFluor™ Violet 450 acid
1140 5 mg
EUR 222

Bottle Centrifuge Pp 450 Ml
3141-0500 PK4
EUR 172.8

AKT1 (Ab-450) Conjugated Antibody
C21502 100ul
EUR 476.4

iFluor® 450 succinimidyl ester
71506 5 mg
EUR 781

iFluor® 450 succinimidyl ester
71556 10 mg
EUR 1301

iFluor® 450 succinimidyl ester
71026 100 ug
EUR 82

Magnetic Beads (DNA) 450 mL
P920-450 - Ask for price

iFluor® 450 succinimidyl ester
71026-100ug 100 ug
EUR 82
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

iFluor® 450 succinimidyl ester
71506-5mg 5 mg
EUR 781
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

iFluor® 450 succinimidyl ester
71556-10mg 10 mg
EUR 1301
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

mFluorâ„¢ Violet 450-streptavidin conjugate
16930-100ug 100 ug
EUR 109
Description: Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.

mFluorâ„¢ Violet 450-VAD-FMK
13475-25Tests 25 Tests
EUR 109
Description: FAM-VAD is a blue fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10.

iFluor® 450 succinimidyl ester
1026-1mg 1 mg
EUR 222
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

TMB Stop Reagent 450 nm
21530071-1 100 mL
EUR 20.55

TMB Stop Reagent 450 nm
21530071-2 500 mL
EUR 43.56

TMB Stop Reagent 450 nm
21530071-3 1 L
EUR 63.61

Akt (Ab-450) polyclonal antibody
E43P0725 100ul
EUR 225
Description: Available in various conjugation types.

Akt (Ab-450) polyclonal antibody
E43SP1810 100ul
EUR 225
Description: Available in various conjugation types.

EFL300 Sieve Shaker
SIE3078 EACH
EUR 3378

Polyclonal CYP24 / CYP24A1 Antibody (aa400-450)
APG02862G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CYP24 / CYP24A1 (aa400-450). This antibody is tested and proven to work in the following applications:

mFluor™ Violet 450-VAD-FMK
13475 25 Tests
EUR 109

mFluor™ Violet 450-streptavidin conjugate
16930 100 ug
EUR 109

mFluorâ„¢ Violet 450-PEG4-Biotin Conjugate
3116-1mg 1 mg
EUR 195
Description: mFluorâ„¢ Violet 450-PEG4-Biotin Conjugate contains mFluorâ„¢ Violet 450 fluorophore that is well excited by the common 405 nm violet laser.

Annexin V-mFluorâ„¢ Violet 450 conjugate
20080-100tests 100 tests
EUR 222
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Polypropylene Magnet Retriever 450 x 10mm
STI5212 EACH
EUR 9.6

Polyclonal TUBB / Beta Tubulin Antibody (aa401-450)
APR13866G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TUBB / Beta Tubulin (aa401-450). This antibody is tested and proven to work in the following applications:

Polyclonal OLFM4 / Olfactomedin 4 Antibody (aa400-450)
APR02466G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human OLFM4 / Olfactomedin 4 (aa400-450). This antibody is tested and proven to work in the following applications:

Annexin V-mFluor™ Violet 450 conjugate
20080 100 tests
EUR 222

Filter for Accuris Microplate Reader, 450 nm
MR9600-450 1 PC
EUR 225.9

CytoCalceinâ„¢ Violet 450 *Excited at 405 nm*
22012-1mg 1 mg
EUR 222
Description: CytoCalcein™ Violet 450 is designed for labeling live cells in the same way to calcein, AM.

Recombinant Salmonella envZ Protein (aa 1-450)
VAng-Wyb0428-inquire inquire Ask for price
Description: Salmonella typhi Osmolarity sensor protein EnvZ, recombinant protein.

Elab Fluor® Violet 450 Labeling Kit
E-LK-E010-10Reaction 10 Reaction
EUR 1000

Elab Fluor® Violet 450 Labeling Kit
E-LK-E010-1Reaction 1 Reaction
EUR 260

Elab Fluor® Violet 450 Labeling Kit
E-LK-E010-3Reaction 3 Reaction
EUR 500

EFL1 Antibody / EFTUD1
F54540-0.08ML 0.08 ml
EUR 140.25
Description: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]

EFL1 Antibody / EFTUD1
F54540-0.4ML 0.4 ml
EUR 322.15
Description: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]

Monoclonal TUBB3 / Tubulin Beta 3 Antibody (aa436-450)
APR13878G 0.05mg
EUR 580.8
Description: A Monoclonal antibody against Human TUBB3 / Tubulin Beta 3 (aa436-450). The antibodies are raised in Mouse. This antibody is applicable in WB and IHC-P, ICC

Polyclonal HTR2C / 5-HT2C Receptor Antibody (aa400-450)
APR07860G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HTR2C / 5-HT2C Receptor (aa400-450). This antibody is tested and proven to work in the following applications:

iFluor® 450 Anti-human CD3 Antibody *HIT3a*
10030040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *HIT3a*
10030041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *HIT3b*
10031040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *HIT3b*
10031041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *UCHT1*
10032040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *UCHT1*
10032041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *SK7*
10033040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *SK7*
10033041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *HIT4a*
10040040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *HIT4a*
10040041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *SK3*
10042040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *SK3*
10042041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *HISM2*
10050040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *HISM2*
10050041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *L17F12*
10051040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *L17F12*
10051041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *ICRF44*
10112040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *ICRF44*
10112041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11c Antibody *3.9*
10113040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11c Antibody *3.9*
10113041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD13 Antibody *WM15*
10130040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD13 Antibody *WM15*
10130041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD14 Antibody *61D3*
10141040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD14 Antibody *61D3*
10141041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *HI149*
10010040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *HI149*
10010041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *SN13*
10012040 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *SN13*
10012041 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *L161*
10013040 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *L161*
10013041 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD2 Antibody *HIT11*
10020040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD2 Antibody *HIT11*
10020041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD21 Antibody *HI21a*
10210040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD21 Antibody *HI21a*
10210041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD22 Antibody *HIB22*
10220040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD22 Antibody *HIB22*
10220041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD24 Antibody *HI45*
10240040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD24 Antibody *HI45*
10240041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *HI25a*
10250040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *HI25a*
10250041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *7G7B6*
10251040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *7G7B6*
10251041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD27 Antibody *LT27*
10270040 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD27 Antibody *LT27*
10270041 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD9 Antibody *HI9a*
10090040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD9 Antibody *HI9a*
10090041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD10 Antibody *HI10a*
10100040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD10 Antibody *HI10a*
10100041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11a Antibody *HI111*
10110040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11a Antibody *HI111*
10110041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *HI11b*
10111040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *HI11b*
10111041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *UCHT2*
10052040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

  1. Within the following, alizarin pink, oil red-O and toluidine blue staining have been carried out to judge the multi-lineage differentiation of zebrafish coronary heart and liver tissue-derived MSCs. Subsequently, the gene and protein expression of Oct4, Sox2 and Nanog as pluri

    -potent markers have been analyzed by RT-PCR and western blotting, respectively.

As well as, MTT assay was used for cell proliferation potential and inhabitants doubling time (PDT) evaluation. Additionally, the growing older of cells was investigated by β-galactosidase exercise assay. The outcomes confirmed that, like different MSCs, zebrafish coronary heart and liver tissue-derived MSCs have been constructive for mesenchymal, adverse for hematopoietic markers and expressed pluripotent markers Oct4, Sox2 and Nanog. Furthermore, these cells have been differentiated to osteocyte, adipocyte, and chondrocyte lineages following directed differentiation. It was discovered that PDT of zebrafish coronary heart and liver tissue-derived MSCs have been 50.67 and 46.61 h, respectively.

These cells had considerably extra speedy progress on day 4. Our outcomes present that zebrafish coronary heart and liver tissue-derived MSCs exhibited typical MSC traits together with fibroblast morphology, multi-lineage differentiation capability, pluriefficiency potential and expression of a typical set of basic MSC floor markers.