Differential gene expression and chemical patterns of an intertidal crab inhabiting a polluted port and an adjacent marine protected area

The acquisition of data to safeguard marine protected areas located close to ports is important in order to develop plans that allow effective protection from pollution as well as sustainable development of the port. The area Secche della Meloria is a Marine Protected Area (MPA-MEL) three miles from Livorno Harbour (LH), which is characterized by a long history of pollution. Here we studied the bioaccumulation and transcriptomic patterns of the marbled crab, Pachygrapsus marmoratus (Fabricius, 1787) (Crustacea; Brachyura, Grapsidae), inhabiting the two selected sites.
Results showed that the two crab populations are significantly different in their chemical composition of trace elements and Polyciclic Aromatic Hydrocarbons (PAHs), and gene expression patterns (1280 DEGs). Enrichment analysis indicated that crabs at LH had the highest stress response genes, and they were associated with higher levels of bioaccumulation detected in body tissues. We are confident that the significant differential gene expression profiles observed between crabs, characterized by https://biodas.org/ significant chemical differences, is associated with responses to contaminant exposure.

Comparison of Perioperative Outcomes Between Single-Port and Multi-Port Robotic Adrenalectomy

Background: Single-port (SP) robotic surgery has been utilized in several surgical procedures. We aim to describe our institution’s approach and perioperative experience with SP robotic adrenalectomy and compare it to the traditional multi-port (MP) approach.
Methods: We retrospectively reviewed all patients who underwent robotic adrenalectomy by a single surgeon between March 2019 and March 2020. Patient demographic, perioperative factors, and pathologic outcomes were recorded and analyzed using t-tests, chi-square, or Fisher’s exact tests.
Results: Thirty-six patients underwent SP (n = 11) and MP (n = 25) robotic adrenalectomy. Age, body mass index, gender, operative time, major Clavien-Dindo complications, and margin status showed no differences. Patients undergoing SP adrenalectomy had a lower estimated blood loss (18.1 ± 13.0 vs 65.6 ± 95.0 cc, P = .02) and smaller lesion size (2.8 ± 1.3 vs 4.1 ± 1.8 cm, P = .04) compared to those undergoing MP.
Conclusions: SP adrenalectomy appears to be a feasible approach in select adrenal masses. Further studies are needed to establish its safety and cost effectiveness.

Analysis of ocular injury 1-year outcome in survivors of Beirut Port ammonium nitrate blast

Purpose: Ascertain the 1-year outcome of patients who sustained open eye injuries from the Beirut Port ammonium nitrate (AN) explosion, one of the most powerful non-nuclear explosions in history.
Methods: Retrospective chart review of the operated eyes in 2 major eye hospitals.
Results: Out of 42 patients with open globe injury that was originally sutured, 29 patients (34 eyes) were followed at the 1-year mark. The initial vision in logMAR (mean ± SD) was 2.93 ± 0.87 (hand motion equivalent) and the final vision was 1.80 ± 1.47 (counting finger 2 m equivalent). No light perception (NLP) vision was noted in 12 eyes on presentation and 10 eyes remained so, while 2 eyes reached light perception (LP) vision. Eight eyes had an intraoperative expulsive choroidal hemorrhage (7 NLP and 1 LP both pre- and postoperatively), and 6 of the 8 developed phthisis. All eyes that developed phthisis had NLP preoperatively and postoperatively. Ocular Trauma Score (OTS) correlated inversely with both initial and final vision (p < 0.001). Zone of injury inversely correlated with initial vision (p = 0.02) and positively with final vision (p < 0.001). Final vision was significantly worse in zone 3 vs. zones 1 and 2 (3.2 ± 0.5) vs. 0.9 ± 1.1) (p < 0.001) injuries, as was the initial vision (3.3 ± 0.5 vs. 2.7 ± 0.8; p = 0.002).
Conclusion: The OTS, which provides prognostic information for serious ocular trauma, also yields valuable prognostic information for AN-associated ocular injuries. Expulsive choroidal hemorrhage and NLP vision at presentation remain very poor prognostic signs.

Da Vinci SP Single-Port Robotic Surgery in Gynecologic Tumors: Single Surgeon’s Initial Experience with 100 Cases

Purpose: To report preliminary experience of single-port robotic surgery using the da Vinci SP surgical system in gynecologic tumors.
Materials and methods: This was a retrospective study on 100 consecutive patients who underwent da Vinci SP single-port robotic surgery between November 2018 and January 2021. All procedures were performed by an experienced gynecologic surgeon using a single 2.5-cm umbilical incision.
Results: Of the 100 cases, the procedures included myomectomy (n=76), hysterectomy (n=2), endometrial cancer surgical staging (n=14), radical hysterectomy (n=3), radical trachelectomy (n=3), and ovarian cystectomy (n=2). None of the cases was converted to robotic multiport or open surgery. The median docking time was 5.0 minutes [interquartile range (IQR), 3.0-7.0], the median console time was 107.5 minutes (IQR, 78.7-155.8), and the median total operation time was 250.0 minutes (IQR, 215.0-310.0). The median estimated blood loss was 50.0 mL (IQR, 30.0-100.0), and the median change in hemoglobin level was 0.8 g/dL (IQR, 0.3-1.3). The median pain scores rated on a numerical rating scale immediately after and at 6, 12, and 24 hours after surgery were 5, 2, 2, and 2, respectively. The mean duration of postoperative hospitalization was 2.8 days.
Conclusion: Da Vinci SP single-port robotic surgery was successfully performed in various gynecologic tumors without significant complications. Therefore, this surgical system could be applied in patients who want precise gynecologic surgery while minimizing surgical incision.

Anxiety, Depression and PTSD in Children and Adolescents following the Beirut Port Explosion

Background: On August 4, 2020, Beirut’s port experienced one of the strongest non-nuclear explosions in history, killing approximately 200 people, displacing 300,000 persons, and injuring more than 1000 children.
Methods: An online anonymous survey assessed the prevalence of probable mental health disorders (MHDs) and impact of blast-related and other factors controlling for sociodemographics in 801 children aged 8 to 17 years old.
Results: About two thirds (64%) were screened positive for probable anxiety using the Screen for Childhood Anxiety Related Disorder, 52% for probable PTSD using CRIES-13, and 33% for probable depression using the Mood and Feelings Questionnaire (MFQ). Children who resided farthest way from the explosion site or were not in Beirut during blast had a significantly lower odds of anxiety and PTSD. Children who sustained any physical injury (vs. none) or witnessed casualties (vs. not) were at higher odds for PTSD. Children of parents who reported that their homes sustained minor damages (vs. no damages at all) were at higher odds for anxiety and PTSD, and temporary displacement (vs. none) increased odds of PTSD only. Poorer perceived economic status, poorer academic performance, having a family member injured in the blast, and prior mental health care seeking were associated with higher odds for all MHDs.
Conclusion: Our study, the only one to document the mental health impact of the Beirut Port explosion on children, highlights the critical need for an emergency mental health response, prioritizing disadvantaged communities and children with prior mental health problems.

8-Port Manifolds

9621 Genesee Scientific 10 Manifolds/Unit 40.6 EUR

NBS 6 Port Gassing Manifold - EACH

SHA1124 Scientific Laboratory Supplies EACH 1074.6 EUR

Digitally controlled triple block heater with gas heater, no manifolds. 115V

109A11-09951 Glascol each 3274 EUR

Kinesis Manifold Body 6 Port 1/8in - EACH

CHR3821 Scientific Laboratory Supplies EACH 349.65 EUR

Kinesis Manifold Assy 6 Port 1/16in - EACH

CHR3817 Scientific Laboratory Supplies EACH 380.7 EUR

Kinesis Manifold Assembly 5 Port; 1/16; PEEK

CHR3825 Scientific Laboratory Supplies EACH 183.18 EUR

Kinesis Manifold Body 7 Port; 1/16; PEEK - EACH

CHR3813 Scientific Laboratory Supplies EACH 166.05 EUR

Kinesis Manifold Assembly 7 Port; 1/16; PEEK - EACH

CHR3811 Scientific Laboratory Supplies EACH 172.8 EUR

Kinesis Manifold Assembly 7 Port; 1/8; PEEK - EACH

CHR3815 Scientific Laboratory Supplies EACH 171.45 EUR

Kinesis Manifold Assembly 6 Port; 1/8; PEEK - EACH

CHR3819 Scientific Laboratory Supplies EACH 157.95 EUR

Kinesis Manifold Assembly 5 Port; 1/16; PEEK - EACH

CHR3823 Scientific Laboratory Supplies EACH 194.4 EUR

Kinesis Manifold Assembly 5 Port; 1/8; PEEK - EACH

CHR3827 Scientific Laboratory Supplies EACH 144.45 EUR

Kinesis Manifold Assembly 9 Port; 1/8; PEEK - EACH

CHR3831 Scientific Laboratory Supplies EACH 224.1 EUR

Kinesis Manifold Assembly 9 Port; 1/16; PEEK - EACH

CHR3833 Scientific Laboratory Supplies EACH 261.9 EUR

SmartWasher™ 96 Microplate Washer for 96 Well Plates, manifolds not included, 115V

MW9600 Benchmark Scientific 1 each 5109.49 EUR

SmartWasher™ 96 Microplate Washer for 96 Well Plates, manifolds not included, 230V

MW9600-E Benchmark Scientific 1 each 5109.49 EUR

Kinesis Manifold Assembly 7 Port; 1/16; PEEK; 0.02 (0.5mm) Bore - EACH

CHR3829 Scientific Laboratory Supplies EACH 207.9 EUR

J Young Gpe Dual Bank Manifold With JY Valves 3 Port 300mm Approx Width

NMR1393 Scientific Laboratory Supplies EACH 741 EUR

J Young Gpe Dual Bank Manifold With JY Valves 4 Port 400mm Approx Width

NMR1395 Scientific Laboratory Supplies EACH 905.16 EUR

J Young Gpe Dual Bank Manifold With JY Valves 5 Port 500mm Approx Width

NMR1397 Scientific Laboratory Supplies EACH 1078.44 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY Valves And Vacuum Gauge Port 3 Port 300mm Approx Width

NMR1381 Scientific Laboratory Supplies EACH 449.16 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY Valves And Vacuum Gauge Port 4 Port 400mm Approx Width

NMR1383 Scientific Laboratory Supplies EACH 538.08 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY Valves And Vacuum Gauge Port 5 Port 500mm Approx Width

NMR1385 Scientific Laboratory Supplies EACH 628.14 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY High Performance Valves And Vacuum Gauge Port 3 Port 300mm Approx Width

NMR1382 Scientific Laboratory Supplies EACH 499.32 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY High Performance Valves And Vacuum Gauge Port 4 Port 400mm Approx Width

NMR1384 Scientific Laboratory Supplies EACH 606.48 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY High Performance Valves And Vacuum Gauge Port 5 Port 500mm Approx Width

NMR1386 Scientific Laboratory Supplies EACH 713.64 EUR

J Young Gpe Dual Bank Manifold With JY High Performance Valves 3 Port 300mm Approx Width

NMR1394 Scientific Laboratory Supplies EACH 784.32 EUR

J Young Gpe Dual Bank Manifold With JY High Performance Valves 4 Port 400mm Approx Width

NMR1396 Scientific Laboratory Supplies EACH 970.14 EUR

Comparative thermoresistance of two biological indicators for monitoring steam autoclaves. 3. Comparison performed at 121 degrees C in a hospital prevacuum steam sterilizer

According to Pharmacopoea Nordica, steam autoclaves should be regularly monitored by a specific Swedish preparation of Bacillus stearothermophilus spores. If another biological indicator (BI) is used for such a control, it should first be calibrated against the Swedish BI (SBI) and the two BIs should be equally thermoresistant. Attest No. 1262 BI (ABI) has previously been shown to be more thermoresistant than the SBI at 134 degrees C, saturated steam. The purpose of the present study was to compare the thermoresistance of the SBI and the ABI at 121 degrees C, saturated steam and prevacuum. Seven hundred and twenty units of each BI were heat-exposed in an Emmer 760 litre prevacuum, pressure-pulsing steam autoclave.
After prevacuum with steam injection (manual or automatic preconditioning), the following incremental heat exposure times were used in triplicate (20 simultaneously tested units of each BI in each cycle) according to a randomized scheme: 5, 6 1/2, 8, 9 1/2, 11, 12 1/2, 14 and 15 min. The intra-chamber pressure and temperature were continuously monitored throughout the test and equilibration cycles.
The heat-exposed BI units were cultivated and read as recommended by the manufacturers. SBI and ABI showed a survival-time of 8 min and 11 min respectively, and a kill-time between 14 min and 15 min for both BIs. Thus, the ABI had the narrower survival-kill window. Probit analysis testing of the results showed that the difference in thermoresistance, at 121 degrees C, saturated steam and https://biodas.org/ prevacuum between Attest No. 1262 BI and the Swedish BI mentioned in Pharmacopea Nordica was not statistically significant.

Comparative thermoresistance of two biological indicators for monitoring steam autoclaves. 2. Comparison performed at 134 degrees C in a hospital prevacuum steam sterilizer

The thermoresistance of various lots of two biological indicators (BIs) for steam sterilization control, a Scandinavian BI (SBI) and the Attest BI (ABI), were compared during sterilization cycles in a hospital prevacuum (pressure-pulsing) steam autoclave at 134 degrees C, saturated steam. ABI No. 1242, ABI No. 1262 (its replacement) and incremental heat exposure times between 0 s and 180 s were used. The intrachamber temperature and pressure were continuously measured and monitored throughout the sterilization cycles.
The results showed that both of the ABIs were more thermoresistant than the SBI, giving 33.1% (ABI No. 1242), 18.9% (ABI No. 1262), and 0% (SBI) autoclave survivors. Because the time needed to reach 134 degrees C (preconditioning time) increased as the day progressed, and varied from day to day, correlation between individual incremental heat exposure times and the number of surviving BI units was not possible. Standardized test conditions are necessary for a true comparison of BIs.

Ozone: A Novel Sterilizer for Personal Protective Equipment

Objective: Personal protective equipment (PPE) is urgently sought during public health crises. It is necessary for the safety of both the patient and the healthcare professional. Yet during the recent COVID-19 pandemic, PPE scarcity in many countries, including the United States, has impacted the level of care for patients and the safety of healthcare personnel. Additionally, the implementation of mandatory mask mandates for the general public in many countries forced individuals to either reuse PPE, which can contribute to poor hygiene, or buy PPE in bulk and thereby contribute to the scarcity of PPE. In this study, we investigate the possibility of using a cost-effective ozone sterilization unit on contaminated N95 masks as an alternative to current sterilization methods.
Method: This protocol examined ozone’s ability to decontaminate N95 mask fabric that was exposed to a surrogate virus (Escherichia coli bacteriophage MS2). Once the sterilization unit achieves an ozone concentration of ~30 ppm, a 60-minute or 120-minute sterilization cycle commences. Following the sterilization cycle, we investigated the amount of viable virus on the slide using a viral plaque assay and compared it to a non-sterilized, control slide. Furthermore, we carried out trials to investigate the safety of an ozone sterilization device, by measuring the levels of ozone exposure that individuals may experience when operating the sterilization unit post-cycle.
Results: We showed that a 120-minute sterilization cycle at ~30 ppm achieves a 3-log reduction in viral activity, thereby complying with industry and U.S. Food and Drug Administration (FDA) standards. Further, we demonstrated that when following our protocol, the ozone exposure levels for a simple sterilization unit to be used at home complied with federal and industry standards.
Conclusion: Ozone may have the potential to decontaminate masks and other PPE.

Monitoring the Effective Sterilization of Low-Temperature Hydrogen Peroxide Gas Plasma Sterilizers in 58 Hospitals – 22 PLADs, China, June 2015-December 2019

What is already known on this topic?: Hydrogen peroxide sterilizeation is widely used for luminal devices. However, the low penetrability of the sterilant is of major concern.
What is added by this report?: This report investigated the effective sterilization of low-temperature hydrogen peroxide gas plasma sterilizers and compared the applicability of different biological monitoring methods based on medical luminal devices.
What are the implications for public health practice?: It is recommended to use a biological process challenge device for monitoring the sterilization of luminal devices with low-temperature hydrogen peroxide gas plasma sterilizers.

Portable sterilizer with microbe content detection device

  • Background: Infectious diseases, such as the latest COVID pandemic, caused by microorganisms like bacteria and virus, wreak havoc shaking human civilization with its rapid infection rate, and high number of mortalities. In case of a contagious disease, the virus can survive on any surface over a period of time and can be transferred to the human host through touching those surfaces unknowingly. Cleaning those possible surfaces to which these microorganisms can cling onto is one of the major ways to curb the spread. The aim of this study was to design a sterilizer which can clean such surfaces of daily used items easily within a certain period of time and can assess the cleaning efficacy by estimating the presence of microbes before and after sanitization.
  • Method development: To achieve this goal, we propose a portable sterilization unit that contains a sterilization chamber fitted with a microbe content detector. The sterilization chamber will cleanse the surfaces off the microbes using ultraviolet radiation. The chamber can be portable and at the same time big enough to accommodate items of daily use, like watch, wallet, clothes, utensils to even foods for single-house application. The microbe content detector will detect the success of the sterilization procedure by examining the time-lapse laser speckle images captured by a high-speed camera by mean of image processing algorithm, such that the user can determine whether further sterilization is required.
  • Conclusions: Microbe content detection device associated with the conventional sterilization procedure will give an assessment of the effectiveness of the sterilization. Successful implementation of sterilization for a wide variety of items of everyday use aided with microbe content detection technique is first of its kind and should be an effective tool for use in large communities, offices and public places for effective sterilization to help fight against the spread of infectious diseases.

Integrated Pre-heated 9kw boiler for Rodwell autoclaves for Rodwell autoclaves - EACH

AUT1379 Scientific Laboratory Supplies EACH 5866.39 EUR

Printer for Media Autoclaves - EACH

AUT4519 Scientific Laboratory Supplies EACH 500.86 EUR

Automatic Fill for Rodwell autoclaves - EACH

AUT1349 Scientific Laboratory Supplies EACH 1632.15 EUR

Shelf Support for Rodwell autoclaves - EACH

AUT1374 Scientific Laboratory Supplies EACH 1065.15 EUR

Fan Assisted Cooling for Rodwell autoclaves

AUT1357 Scientific Laboratory Supplies EACH 544.2 EUR

Condensate removal for Rodwell autoclaves - EACH

AUT1381 Scientific Laboratory Supplies EACH 1526.85 EUR

Condensate Collector for Rodwell autoclaves - EACH

AUT2019 Scientific Laboratory Supplies EACH 392.85 EUR

Printer 2 channel for Rodwell autoclaves - EACH

AUT1272 Scientific Laboratory Supplies EACH 1382.4 EUR

Oil Free Compressor for Rodwell autoclaves - EACH

AUT1376 Scientific Laboratory Supplies EACH 2926.8 EUR

Fan assisted cooling for Rodwell autoclaves - EACH

AUT1356 Scientific Laboratory Supplies EACH 1473.21 EUR

Drain Line Condenser for Rodwell autoclaves - EACH

AUT1358 Scientific Laboratory Supplies EACH 1518.75 EUR

Under Chamber Boiler for Rodwell autoclaves - EACH

AUT1377 Scientific Laboratory Supplies EACH 4847.04 EUR

Loading step for Ensign for Rodwell autoclaves - EACH

AUT1350 Scientific Laboratory Supplies EACH 735.75 EUR

Discard System for Genesis for Rodwell autoclaves - EACH

AUT2000 Scientific Laboratory Supplies EACH 1159.65 EUR

2 channel + pressure printer for Rodwell autoclaves - EACH

AUT1274 Scientific Laboratory Supplies EACH 1926.45 EUR

Automatic FillDrain for Clean Steam for Rodwell autoclaves - EACH

AUT1351 Scientific Laboratory Supplies EACH 2619 EUR

Discard system for Rodwell Ambassador 100 and Gemini autoclaves (per chamber) - EACH

AUT2007 Scientific Laboratory Supplies EACH 1764.45 EUR

Prestige Autoclave 2100 Extended

AUT4502 Scientific Laboratory Supplies EACH 1504.8 EUR

Sovereign Autoclave 200E - EACH

AUT0507 Scientific Laboratory Supplies EACH 31050 EUR

Sovereign Autoclave 250E - EACH

AUT0509 Scientific Laboratory Supplies EACH 31050 EUR

Prestige Autoclave 2100 Extended+ - EACH

AUT4504 Scientific Laboratory Supplies EACH 2739.31 EUR

Floor Standing Autoclave - 120L - EACH

AUT5000 Scientific Laboratory Supplies EACH 36907.65 EUR

Floor Standing Autoclave - 153L - EACH

AUT5002 Scientific Laboratory Supplies EACH 40979.25 EUR

Floor Standing Autoclave - 247L - EACH

AUT5004 Scientific Laboratory Supplies EACH 45276.3 EUR

Floor Standing Autoclave - 290L - EACH

AUT5006 Scientific Laboratory Supplies EACH 48340.8 EUR

Floor Standing Autoclave - 344L - EACH

AUT5008 Scientific Laboratory Supplies EACH 50566.95 EUR

Evaluation of a Wearable Non-Invasive Thermometer for Monitoring Ear Canal Temperature during Physically Demanding (Outdoor) Work

Aimed at preventing heat strain, health problems, and absenteeism among workers with physically demanding occupations, a continuous, accurate, non-invasive measuring system may help such workers monitor their body (core) temperature. The aim of this study is to evaluate the accuracy and explore the usability of the wearable non-invasive Cosinuss° °Temp thermometer. Ear canal temperature was monitored in 49 workers in real-life working conditions. After individual correction, the results of the laboratory and field study revealed high correlations compared to ear canal infrared thermometry for hospital use. After performance of the real-life working tasks, this correlation was found to be moderate.
It was also observed that the ambient environmental outdoor conditions and personal protective clothing influenced the accuracy and resulted in unrealistic ear canal temperature outliers. It was found that the Cosinuss° °Temp thermometer did not result in significant interference during work. Therefore, it was concluded that, without a correction factor, the Cosinuss° °Temp thermometer is inaccurate. Nevertheless, with a correction factor, the reliability of this wearable ear canal thermometer was confirmed at rest, but not in https://biodas.org/ outdoor working conditions or while wearing a helmet or hearing protection equipment.

Mobile Health-Based Thermometer for Monitoring Wound Healing After Endovascular Therapy in Patients With Chronic Foot Ulcer: Prospective Cohort StudY

Background: Foot temperature may increase after endovascular therapy, but the relationship between foot temperature and wound healing is unclear.
Objective: This study was performed to evaluate the feasibility of a mobile health (mHealth)-based thermometer for foot temperature monitoring in patients with chronic foot ulcer before and after endovascular therapy and to determine the association between temperature change and wound healing time.
Methods: This was a prospective cohort study. Patients who had a chronic foot ulcer (>3 months) and underwent endovascular therapy between June 2019 and December 2019 were included. The participants received standard medical care and endovascular therapy for revascularization. The mHealth-based thermometer, composed of 4 temperature-sensing chips, was put on the foot before and after endovascular therapy. Data from the chips were transferred to an associated mobile phone app via Bluetooth. Wound healing time was estimated using the Kaplan-Meier method, and the associations between baseline characteristics and clinical outcomes were evaluated using a Cox proportional hazard model.
Results: A total of 163 patients with chronic foot ulcer who underwent endovascular therapy were enrolled and followed up until wound healing was complete or for 180 days. The mean foot temperature before endovascular therapy was 30.6 (SD 2.8 °C). Foot temperature increased significantly (mean 32.1 °C, SD 2.8 °C; P=.01) after the procedure. Wound healing time was significantly different in the Kaplan-Meier curves of the patient group with temperature changes ≥2 °C and the group with temperature changes ≤2 °C (log-rank P<.001). A foot temperature increase ≥2 °C after endovascular therapy was associated with increased wound healing in univariate analysis (hazard ratio [HR] 1.78, 95% CI 1.24-2.76, P=.02), and the association remained significant in multivariate analysis (HR 1.69, 95% CI 1.21-2.67, P=.03).
Conclusions: The mHealth-based thermometer was feasible and useful for foot temperature monitoring, which may provide health care professionals with a new endpoint for endovascular therapy. Foot temperature increases ≥2 °C after endovascular therapy were associated with faster wound healing in patients with chronic foot ulcer. Further studies are needed, however, to confirm these findings.

Tympanic thermometers support fast and accurate temperature monitoring in acute and alternative care

This article explores body temperature and the physiological process of thermoregulation. Normal body temperature and body temperature changes are discussed, including comorbidities associated with body temperature and signs of hyperthermia and hypothermia, and the factors that affect intraoperative temperature regulation.
The evidence base behind thermometry is discussed and is applied to contemporary clinical conditions and symptoms, including: sepsis and suspected COVID-19. After discussing clinical considerations and regulations that encompass thermometry, three case studies present the use of the Genius 3 Tympanic Thermometer in clinical practice, with user feedback supporting its benefits, which include speed, accuracy and ease of use.

Mitochondria-Anchored Molecular Thermometer Quantitatively Monitoring Cellular Inflammations

Temperature in mitochondria can be a critical indicator of cell metabolism. Given the highly dynamic and inhomogeneous nature of mitochondria, it remains a big challenge to quantitatively monitor the local temperature changes during different cellular processes. To implement this task, we extend our strategy on mitochondria-anchored thermometers from “on-off” probe Mito-TEM to a ratiometric probe Mito-TEM 2.0 based on the Förster resonance energy transfer mechanism. Mito-TEM 2.0 exhibits not only a sensitive response to temperature through the ratiometric changes of dual emissions but also the specific immobilization in mitochondria via covalent bonds.
Both characters support accurate and reliable detection of local temperature for a long time, even in malfunctioning mitochondria. By applying Mito-TEM 2.0 in fluorescence ratiometric imaging of cells and zebrafishes, we make a breakthrough in the quantitative visualization of mitochondrial temperature rises in different inflammation states.

Non-invasive and wearable thermometer for continuous monitoring of core body temperature under various convective conditions

We describe the design of a thermometer that can be worn during everyday activities for monitoring core body temperature (CBT) at the skin surface. This sensor estimates the CBT by measuring the heat flux from the body core based on a thermal conductive model. The heat flux is usually affected by the ambient convective conditions (e.g. air conditioner or posture), which in turn affects the model’s accuracy. Thus, we analytically investigated heat conduction and designed a sensor interface that would be robust to convection changes. We performed an in vitro experiment and a preliminary in vivo experiment. The accuracy of CBT in an in vitro experiments was 0.1°C for convective values ranging from 0 to 1.2 m/s. The wearable thermometer has high potential as non-invasive CBT monitor.

Effect of monitoring the onset of calving by a calving alarm thermometer on the prevalence of dystocia, stillbirth, retained fetal membranes and clinical metritis in a Hungarian dairy farm

The objective of the present study was to assess the effectiveness of an intravaginal thermometer in the field prediction of the second stage of labor and to determine its impact on the health of dams and newborn calves. Holstein cows (n = 241) were randomly selected about 5 (mean ± SD: 4.7 ± 2.0) days before the expected date of calving and the thermometer was inserted into the vagina. Another 113 cattle served as controls. There was no false alarm during the experiment. The risk of dystocia (Score >1) was 1.9 times higher, the prevalence of stillbirth was 19.8 times higher, the risk of retained fetal membranes (RFM) was 2.8 times higher and the risk of clinical metritis was 10.5 times higher in the control group than in the experimental group.
The prevalence of stillbirth was 7 times higher in cows with dystocia compared to cows with eutocia. The presence of dystocia and stillbirth increased the risk of RFM 4 and 5 times, respectively. The occurrence of RFM increased the risk of development of clinical metritis with a 22 times higher odds. The results indicate that the use of calving alert systems not only facilitates controlling the time of parturition and providing prompt and appropriate calving assistance but also decreases the number of dystocia cases and improves reproductive efficiency, postpartum health of the dam and newborn calf survival.

SOM02.0 Selected Ion Monitoring 2 Components - 1ML

CLPS-SOM-ISB Scientific Laboratory Supplies 1ML 133.65 EUR

THERMOMETER, DIGITAL

T786 PhytoTechnology Laboratories 1EA 84.82 EUR

scanning thermometer

T8710 Consort ea 1538.4 EUR

scanning thermometer

T8720 Consort ea 1538.4 EUR

3M Clean-Trace Hygiene Monitoring and Management System - EACH

FSA1260 Scientific Laboratory Supplies EACH 7275.15 EUR

Thermometer -1 to 101C (0.2)

THE1374 Scientific Laboratory Supplies EACH 18.45 EUR

SOM02.0 Deuterated Monitoring Compounds Standard Spiking Solution - 1ML

CLPS-SOM-ISA Scientific Laboratory Supplies 1ML 1463.4 EUR

Spirit Thermometer -10 to 110C

THE1080 Scientific Laboratory Supplies EACH 3.48 EUR

Thermometer (TDC 150) - EACH

THE1802 Scientific Laboratory Supplies EACH 55.52 EUR

Thermometer/hygrometer - EACH

DD98463 Scientific Laboratory Supplies EACH 29.7 EUR

Thermometer -1 to 101C (0.2) - EACH

THE1372 Scientific Laboratory Supplies EACH 28.23 EUR

Testo Thermometer 110+Battery - EACH

THE2070 Scientific Laboratory Supplies EACH 163.04 EUR

Thermometer -1 to 51C (0.1)

THE1366 Scientific Laboratory Supplies EACH 18.45 EUR

Thermometer -1 to 51C (0.2)

THE1370 Scientific Laboratory Supplies EACH 18.45 EUR

Digital Thermometer Hygrometer

THE1168 Scientific Laboratory Supplies EACH 38.4 EUR

Teflon Thermometer -20 to 110 - EACH

THE1164 Scientific Laboratory Supplies EACH 24.71 EUR

Teflon Thermometer -10 to 210 - EACH

THE1166 Scientific Laboratory Supplies EACH 30.11 EUR

Teflon Thermometer -20 to 150 - EACH

THE1178 Scientific Laboratory Supplies EACH 26.48 EUR

Alarm Thermometer -50 to +150C - EACH

THE1502 Scientific Laboratory Supplies EACH 39.15 EUR

Spirit Thermometer -10 to 110C - EACH

THE1062 Scientific Laboratory Supplies EACH 5.32 EUR

Spirit Thermometer -10 to 150C - EACH

THE1064 Scientific Laboratory Supplies EACH 4.41 EUR

Spirit Thermometer -0 to 240F - EACH

THE1066 Scientific Laboratory Supplies EACH 5.32 EUR

Digital Thermometer -40 to 240 - EACH

THE1174 Scientific Laboratory Supplies EACH 35.4 EUR

Digital Thermometer -50 to 300 - EACH

THE1176 Scientific Laboratory Supplies EACH 67.12 EUR

An overview of different homogenizers, their working mechanisms and impact on processing of fruits and vegetables

Fruits and vegetables (F&V) are the second highest recommended foods, rich in antioxidants, vitamins and minerals, vital for building immunity against chronic diseases. F&V processing involves particle size reduction, for which different types of homogenizers, categorized as mechanical homogenizers, pressure homogenizers and ultrasonic homogenizers are used. The review discusses different types of homogenizers, their working mechanism, and application in F&V processing. Among mechanical homogenizers, knife mills are used for primary size reduction, ball mills for the micronization of dried F&V and rotor-stator homogenizers for emulsification.
Use of the ultrasonic homogenizer is limited to extraction of bioactive compounds or as a pre-treatment for dehydration of F&V. High-pressure homogenizers are most widely used and reported due to the synergistic effect of homogenization and temperature increase, resulting in longer shelf-life and better physicochemical properties of the product. Additionally, the review also explains the effect of homogenization on the physicochemical, sensory and https://biodas.org/ nutraceutical properties of the product.

Pre-processing tissue specimens with a tissue homogenizer: clinical and microbiological evaluation

Background: Tissues are valuable specimens in diagnostic microbiology because they are often obtained by invasive methods, and effort should thus be taken to maximize microbiological yield. The objective of this study was to evaluate the added value of using tissue pre-processing (tissue homogenizer instrument gentleMACS Dissociator) in detecting microorganisms responsible for infections.
Methods: We included 104 randomly collected tissue samples, 41 (39.4 %) bones and 63 (60.6 %) soft tissues, many of those (42/104 (40.4 %)) were of periprosthetic origins. We compared the agreement between pre-processing tissues using tissue homogenizer with routine microbiology diagnostic procedure, and we calculated the performance of these methods when clinical infections were used as reference standard.
Results: There was no significant difference between the two methods (McNemar test, p = 0.3). Among the positive culture using both methods (n = 62), 61 (98.4 %) showed at least one similar microorganism. Exactly similar microorganisms were found in 42/62 (67.7 %) of the samples. From the included tissues, 55/ 104 (52.9 %) were deemed as infected. We found that the sensitivity of homogenized tissue procedure was lower (83.6 %) than when tissue was processed using tissue homogenizer (89.1 %). Sub-analysis on periprosthetic tissues and soft or bone tissues showed comparable results.
Conclusions: The added value of GentleMACS Dissociator tissue homogenizer is limited in comparison to routine tissue processing.

Functionality of MC88- and MPC85-Enriched Skim Milk: Impact of Shear Conditions in Rotor/Stator Systems and High-Pressure Homogenizers on Powder Solubility and Rennet Gelation Behavior

  • Milk protein concentrate (MPC) and micellar casein (MC) powders are commonly used to increase the protein concentration of cheese milk. However, highly-concentrated milk protein powders are challenging in terms of solubility. The research question was whether and how incompletely dissolved agglomerates affect the protein functionality in terms of rennet gelation behavior. For the experiments, skim milk was enriched with either MC88 or MPC85 to a casein concentration of 4.5% (w/w) and sheared on a laboratory and pilot scale in rotor/stator systems (colloid mill and shear pump, respectively) and high-pressure homogenizers.
  • The assessment criteria were on the one hand particle sizes as a function of shear rate, and on the other hand, the rennet gelation properties meaning gelling time, gel strength, structure loss upon deformation, and serum loss. Furthermore, the casein, whey protein, and casein macropeptide (CMP) recovery in the sweet whey was determined to evaluate the shear-, and hence, the particle size-dependent protein accessibility. We showed that insufficient powder rehydration prolongs the rennet gelation time, leading to softer, weaker gels, and to lower amounts of CMP and whey protein in the sweet whey.

Characterization of Astaxanthin Nanoemulsions Produced by Intense Fluid Shear through a Self-Throttling Nanometer Range Annular Orifice Valve-Based High-Pressure Homogenizer

Stable, oil-in-water nanoemulsions containing astaxanthin (AsX) were produced by intense fluid shear forces resulting from pumping a coarse reagent emulsion through a self-throttling annular gap valve at 300 MPa. Compared to crude emulsions prepared by conventional homogenization, a size reduction of over two orders of magnitude was observed for AsX-encapsulated oil droplets following just one pass through the annular valve. In krill oil formulations, the mean hydrodynamic diameter of lipid particles was reduced to 60 nm after only two passes through the valve and reached a minimal size of 24 nm after eight passes.
Repeated processing of samples through the valve progressively decreased lipid particle size, with an inflection in the rate of particle size reduction generally observed after 2-4 passes. Krill- and argan oil-based nanoemulsions were produced using an Ultra Shear Technology™ (UST™) approach and characterized in terms of their small particle size, low polydispersity, and stability.

Characteristics of an Emulsion Obtained Using Hydrophobic Hydroxypropyl Methylcellulose as an Emulsifier and a High-Pressure Homogenizer

Hydrophobically modified hydroxypropyl methylcellulose (HM-HPMC), a polymer in which a small amount of HPMC is stearoxyl substituted, was used as an emulsifier of emulsion-type lotion. A high-pressure homogenizer (microfluidizer) was used. The viscosity of the 1% HM-HPMC aqueous gel decreased after passing through the microfluidizer from 5.5 to 2.7 Pa·s. When liquid paraffin (LP) was used as the oil phase, a stable emulsion was obtained with an LP ratio of 1-40%. The apparent viscosity decreased with LP ratios up to 20%, and then increased with increasing LP concentration.
The emulsions with an LP ratio <20% presented a pseudo-viscous flow, similar to that of the diluted polymer solution. HM-HPMC likely adsorbed onto the oil with a stearoxyl group; thus, the interaction between the stearoxyl group, which explained the high viscosity of HM-HPMC, decreased, reducing the viscosity of the emulsion. The LP ratio was 40%, and the emulsion presented a plastic flow, which is typical of concentrated emulsions. The size of the droplet in the emulsion was approximately 1 µm regardless of the LP ratio. When low-viscosity LPs or monoester-type oils such as isopropyl myristate were used, some of the emulsions presented creaming. An emulsion using HM-HPMC as an emulsifier and an appropriate oil homogenized with a microfluidizer is stable, has low viscosity, and can be easily spread on skin.

Proteomic evaluation of plasma membrane fraction prepared from mouse liver and kidney using a bead homogenizer: Enrichment of drug-related transporter proteins

Quantifying the protein levels of drug transporters in plasma membrane fraction helps elucidate the function of these transporters. In this study, we conducted a proteomic evaluation of enriched drug-related transporter proteins in plasma membrane fraction prepared from mouse liver and kidney tissues using the Membrane Protein Extraction Kit and a bead homogenizer. Crude and plasma membrane fractions were prepared using either the Dounce or bead homogenizer, and protein levels were determined using quantitative proteomics.
In liver tissues, the plasma membrane fractions were more enriched in transporter proteins than the crude membrane fractions; the average enrichment ratios of plasma-to-crude membrane fractions were 3.31 and 6.93 using the Dounce and bead homogenizers, respectively. The concentrations of transporter proteins in plasma membrane fractions determined using the bead homogenizer were higher than those determined using the Dounce homogenizer. Meanwhile, in kidney tissues, the plasma membrane fractions were enriched in transporters localized in the brush-border membrane to the same degree for both the homogenizers; however, the membrane fractions obtained using either homogenizer were not enriched in Na+/K+-ATPase and transporters localized in the basolateral membrane. These results indicate that fractionation, using the bead homogenizer, yielded transporter-enriched plasma membrane fractions from mouse liver and kidney tissues; however, no enrichment of basolateral transporters was observed in plasma membrane fractions prepared from kidney tissues.

Ceramic grinding bars 3/8X5/8, 45°, angled medium ceramic homogenizers pack of 100

IPD9600-3858-1 Benchmark Scientific each 39.14 EUR

Ceramic grinding bars 3/8X7/8 , 45°, angled medium ceramic homogenizers pack of 100

IPD9600-3878-1 Benchmark Scientific each 39.14 EUR

BeadBug™ Microtube homogenizer, 115V

D1030 Benchmark Scientific 1 each 1000.42 EUR

BeadBug™ Microtube homogenizer, 230V

D1030-E Benchmark Scientific 1 PC 1000.42 EUR

BeadBug 6, Six Position Homogenizer, 115V

D1036 Benchmark Scientific 1 each 2696.23 EUR

BeadBug 6, Six Position Homogenizer, 230V

D1036-E Benchmark Scientific 1 PC 2696.23 EUR

BeadBug 6 Six Position Homogenizer 230V - EACH

HOM3018 Scientific Laboratory Supplies EACH 3825.9 EUR

BeadBlaster 96 Ball Mill Homogenizer, 120V US Plug

IPD9600 Benchmark Scientific each 11892.18 EUR

BeadBlaster 96 Ball Mill Homogenizer, 230V EU Plug

IPD9600-E Benchmark Scientific each 11892.18 EUR

BeadBlaster™ Microtube homogenizer, 115V

D2400 Benchmark Scientific 1 each 9460.6 EUR

BeadBlaster™ Microtube homogenizer, 230V

D2400-E Benchmark Scientific 1 PC 9460.6 EUR

BeadBlaster Microtube homogenizer 230V - EACH

HOM3012 Scientific Laboratory Supplies EACH 13678.2 EUR

BeadBug Microtube homogenizer - EACH

SLS1402 Scientific Laboratory Supplies EACH 1629.45 EUR

BeadBlaster™ 24 Refrigerated Microtube Homogenizer, 115V

D2400-R Benchmark Scientific 1 each 15098.14 EUR

BeadBlaster™ 24 Refrigerated Microtube Homogenizer, 230V

D2400-R-E Benchmark Scientific 1 each 15098.14 EUR

BeadBlaster 24 Refrigerated Microtube Homogenizer 230V - EACH

HOM3078 Scientific Laboratory Supplies EACH 24792.75 EUR

Homogenizer stand for Agile™ Hand-held homogenizer

AHM1 ACTGene VS 414.21 EUR

Homogenizer stand for Agile? Hand-held homogenizer

AHM1-VS ACTGene each 634.8 EUR

Microtube homogenizer, 115V

BCM1200 Bio Basic 1 pcs, 1 UNIT 11944.61 EUR

Microtube homogenizer, 115V

BCM1201 Bio Basic 1 pcs, 1 UNIT 1224.14 EUR

An Economical, Portable Manual Cryogenic Plunge Freezer for the Preparation of Vitrified Biological Samples for Cryogenic Electron Microscopy.

Visualizing biological structures and cellular processes in their native state is a major goal of many scientific laboratories. In the past 20 years, the technique of preserving samples by vitrification has greatly expanded, specifically for use in cryogenic electron microscopy (cryo-EM). Here, we report on improvements in the design and use of a portable manual cryogenic plunge freezer that is intended for use in laboratories that are not equipped for the cryopreservation of samples.

The construction of the instrument is economical, can be produced by a local machine shop without specialized equipment, and lowers the entry barriers for newcomers with a reliable alternative to costly commercial equipment. The improved design allows for successful freezing of isolated proteins for single particle analysis https://biodas.org/ as well as bacterial cells for cryo-electron tomography. With this instrument, groups will be able to prepare vitreous samples whenever and wherever necessary, which can then be imaged at local or national cryo-EM facilities.

Successful short-term cryopreservation of volume-reduced cord blood units in a cryogenic mechanical freezer: effects on cell recovery, viability, and clonogenic potential

BACKGROUND
Cord blood (CB) units are stored from weeks to years in liquid- or vapor-phase nitrogen until they are used for transplantation. We examined the effects of cryostorage in a mechanical freezer at -150°C on critical quality control variables of CB collections to investigate the possible use of mechanical freezers at -150°C as an alternative to storage in liquid- (or vapor-) phase nitrogen.
METHODS
A total of 105 CB units were thawed and washed at different time intervals (6, 12, 24, and 36 months). For every thawed CB unit, samples were removed and cell enumeration (total nucleated cells [TNCs], mononuclear cells [MNCs], CD34+, CD133+) was performed. In addition, viability was obtained with the use of flow cytometry, and recoveries were calculated. Also, total absolute colony-forming unit counts were performed and progenitor cell recoveries were studied by clonogenic assays.
RESULTS
Significant differences (p < 0.05) were observed in certain variables (TNCs, MNC numbers, viability) when they were examined in relation with time intervals, while others (CD34+, CD133+) were relatively insensitive (p = NS) to the duration of time interval the CB units were kept in cryostorage condition.
CONCLUSIONS
The data presented suggest that cryopreservation of CB units in a mechanical freezer at -150°C may represent an alternative cryostorage condition for CB cryopreservation.

Realignment-free cryogenic macroscopic optical cavity coupled to an optical fiber

We present a cryogenic setup where an optical Fabry-Perot resonator is coupled to a single-mode optical fiber with coupling efficiency above 90% at mK temperatures without realignment during cooling down. The setup is prealigned at room temperature to compensate for the thermal contraction and change of the refractive index of the optical components during cooling down.

The high coupling efficiency is achieved by keeping the setup rotation-symmetric around the optical axis. The majority of the setup components are made of Invar (FeNi36), which minimizes the thermal contraction. High coupling efficiency is essential in quantum optomechanical experiments.

Extraordinary approach to further boost plasmonic NIR-SERS by cryogenic temperature-suppressed non-radiative recombination

We report an effective strategy to promote the near-infrared surface-enhanced Raman scattering spectroscopy (NIR-SERS) activity by boosting the photon-induced charge transfer (PICT) efficiency at cryogenic temperature. Based on as-prepared Au/Ag nano-urchins (NUs) with abundant surface defects, the extremely low temperature (77 K) can significantly weaken the metallic lattice vibration and reduce the recombination of thermal phonons and photoexcited electrons, then accelerate the migration of energetic electrons.
It enables the NIR-SERS detection limit of dye molecules to be achieved at 10-17 M, which is nearly three orders of magnitude better than that at room temperature. The present work provides a new, to the best of our knowledge, approach for ultra-trace NIR-SERS bioanalysis.

Ultra-stretchable and fast self-healing ionic hydrogel in cryogenic environments for artificial nerve fiber

Self-healing materials behave irreplaceable advantages in biomimetic intelligent robots (BIR) for avoiding or reducing safety hazards and economic losses from accidental damage during service. However, the self-healing ability is unreservedly lost and even becomes rigid, fragile in the cryogenic environment where BIR is precisely needed. Here, we report a versatile ionic hydrogel with fast self-healing ability, ultra-stretchability, and stable conductivity, even at -80℃.
The hydrogel is systematically optimized to improve hydrogen-bonded network nanostructure, coordinated achieving a quick self-healing ability within 10 min, large deformation tolerance of over 7000%, superior conductivity of 11.76 S·cm-1 and anti-freezing ability, which is difficult to obtain simultaneously. Such hydrogel provides new opportunities for artificial electronic devices in harsh environments. As a prospective application, we fabricate an artificial nerve fiber by mimicking the structure and functions of the myelinated axon, exhibiting the property of fast and potential-gated signal transmission.
This artificial nerve fiber is integrated into a robot for demonstrating a real-time high fidelity and high throughput information interaction under big deformation and cryogenic temperature. The hydrogel and bionic device will bring pioneering functions for robots and open a broad application scenario in extreme conditions. This article is protected by copyright. All rights reserved.

Cryogenic temperature sensing based on the temperature dependence of color centers in optical fibers

A cryogenic temperature sensor based on the temperature dependence of stable color centers in a commercial single-mode optical fiber is proposed. The radiation induced attenuation spectra at different temperatures are measured and decomposed by Ge-NBOHC and Ge(X) color centers. The configurational coordinate model is used to explain the temperature properties of the color centers.
A series of experiments are conducted to evaluate its performance in the temperature range from 10°C to -196°C, and the results suggest that the temperature sensitivity is ∼0.17 dB/km/°C with a resolution of 0.034°C, and the nonlinearity and repeatability error are ±3.8% and 1.4%, respectively.

Revealing the Intrinsic Atomic Structure and Chemistry of Amorphous LiO 2-Containing Products in Li-O 2 Batteries Using Cryogenic Electron Microscopy

Aprotic lithium-oxygen batteries (LOBs) are promising energy storage systems characterized by ultrahigh theoretical energy density. Extensive research has been devoted to this battery technology, yet the detailed operational mechanisms involved, particularly unambiguous identification of various discharge products and their specific distributions, are still unknown or are subjects of controversy. This is partly because of the intrinsic complexity of the battery chemistry but also because of the lack of atomic-level insight into the oxygen electrodes acquired via reliable techniques. In the current study, it is demonstrated that electron beam irradiation could induce crystallization of amorphous discharge products. Cryogenic conditions and a low beam dosage have to be used for reliable transmission electron microscopy (TEM) characterization.
High-resolution cryo-TEM and electron energy loss spectroscopy (EELS) analysis of toroidal discharge particles unambiguously identified the discharge products as a dominating amorphous LiO2 phase with only a small amount of nanocrystalline Li2O2 islands dispersed in it. In addition, uniform mixing of carbon-containing byproducts is identified in the discharge particles with cryo-EELS, which leads to a slightly higher charging potential. The discharge products can be reversibly cycled, with no visible residue after full recharge. We believe that the amorphous superoxide dominating discharge particles can lead researchers to reconsider the chemistry of LOBs and pay special attention to exclude beam-induced artifacts in traditional TEM characterizations.

10K Cryogenic Freezer With CS200 Controller and Gas ByPass

TW-10K-CS200-GBP MiTeGen 1 UNIT 19442 EUR

24K Cryogenic Freezer With CS200 Controller and Gas ByPass

TW-24K-CS200-GBP MiTeGen 1 UNIT 22879 EUR

38K Cryogenic Freezer With CS200 Controller and Gas ByPass

TW-38K-CS200-GBP MiTeGen 1 UNIT 31556 EUR

80K Cryogenic Freezer With CS200 Controller and Double Step

TW-LABS80K-CS-DS MiTeGen 1 UNIT 64636 EUR

80K Cryogenic Freezer With CS200 Controller and Locking Step

TW-LABS80K-CS MiTeGen 1 UNIT 63686 EUR

94K Cryogenic Freezer With CS200 Controller and Double Locking Steps and Fill Hose with Adapter

TW-LABS94K-SP MiTeGen 1 UNIT 71752 EUR

10K Cryogenic Freezer No Controller

TW-10K MiTeGen 1 UNIT 14003 EUR

24K Cryogenic Freezer No Controller

TW-24K MiTeGen 1 UNIT 19318 EUR

38K Cryogenic Freezer No Controller

TW-38K MiTeGen 1 UNIT 28465 EUR

10K Cryogenic Freezer With CS100 Controller

TW-10K-CS100 MiTeGen 1 UNIT 17922 EUR

10K Cryogenic Freezer With CS200 Controller

TW-10K-CS200 MiTeGen 1 UNIT 18872 EUR

24K Cryogenic Freezer With CS100 Controller

TW-24K-CS100 MiTeGen 1 UNIT 21359 EUR

24K Cryogenic Freezer With CS200 Controller

TW-24K-CS200 MiTeGen 1 UNIT 22309 EUR

38K Cryogenic Freezer With CS200 Controller

TW-38K-CS200 MiTeGen 1 UNIT 30606 EUR

20K Cryogenic Freezer With CS200 Controller

TW-LABS20K-CS MiTeGen 1 UNIT 29839 EUR

40K Cryogenic Freezer With CS200 Controller

TW-LABS40K-CS MiTeGen 1 UNIT 41579 EUR

3K Cryogenic Freezer With Stainless Steel Exterior

TW-3KSBL MiTeGen 1 UNIT 3870 EUR

Special cryogenics label 38 x 19 mm white colour - PK1200

DD53075 Scientific Laboratory Supplies PK1200 180.9 EUR

Special cryogenics label 33 x 13 mm assorted colours - PK1700

DD53520 Scientific Laboratory Supplies PK1700 180.9 EUR

Special cryogenics label 38 x 6 mm assorted colours - PK3120

DD53526 Scientific Laboratory Supplies PK3120 174.15 EUR

CO2 Back Up for ULT Freezers - EACH

SLS1072 Scientific Laboratory Supplies EACH 3414.15 EUR

COOLCELL® LX-4 PACK, 4 COLOURS, CELL FREEZING CONTAINER, FOR 12 X 1ML OR 2ML CRYOGENIC VIALS

432138 CORNING 1/pk 714 EUR

NBS CO2 Backup for Innova Freezers - EACH

FRE6110 Scientific Laboratory Supplies EACH 2515.05 EUR

NBS LN2 Backup for Innova Freezers - EACH

FRE6114 Scientific Laboratory Supplies EACH 2515.05 EUR

NBS CO2 Backup for Premium Freezers - EACH

FRE6112 Scientific Laboratory Supplies EACH 2336.85 EUR

NBS LN2 Backup for Premium Freezers - EACH

FRE6116 Scientific Laboratory Supplies EACH 2515.05 EUR