Validity evidence for an instrument for cognitive load for virtual didactic sessions

Background: COVID necessitated the shift to virtual resident instruction. The challenge of learning via virtual modalities has the potential to increase cognitive load. It is important for educators to reduce cognitive load to optimize learning, yet there are few available tools to measure cognitive load. The objective of this study is to identify and provide validity evidence following Messicks’ framework for an instrument to evaluate cognitive load in virtual emergency medicine didactic sessions.
Methods: This study followed Messicks’ framework for validity including content, response process, internal structure, and relationship to other variables. Content validity evidence included: (1) engagement of reference librarian and literature review of existing instruments; (2) engagement of experts in cognitive load, and relevant stakeholders to review the literature and choose an instrument appropriate to measure cognitive load in EM didactic presentations. Response process validity was gathered using the format and anchors of instruments with previous validity evidence and piloting amongst the author group. A lecture was provided by one faculty to four residency programs via ZoomTM. Afterwards, residents completed the cognitive load instrument. Descriptive statistics were collected; Cronbach’s alpha assessed internal consistency of the instrument; and correlation for relationship to other variables (quality of lecture).
Results: The 10-item Leppink Cognitive Load instrument was selected with attention to content and response process validity evidence. Internal structure of the instrument was good (Cronbach’s alpha = 0.80). Subscales performed well-intrinsic load (α = 0.96, excellent), extrinsic load (α = 0.89, good), and germane load (α = 0.97, excellent). Five of the items were correlated with overall quality of lecture (< 0.05).
Conclusions: The 10-item Cognitive Load instrument demonstrated good validity evidence to measure cognitive load and the subdomains of intrinsic, extraneous, and germane load. This instrument can be https://biodas.org/ used to provide feedback to presenters to improve the cognitive load of their presentations.

Assessment of the influence of gluten quality on highland barley dough sheet quality by different instruments

  • This study was to compare the results of texture analyzer with those of farinograph and extensograph and determine whether texture analyzer could be used to evaluate the processing quality of highland barley flour (HBF) dough sheet. The farinograph and extensograph tests were used to determine the reconstituted flour properties, a texture analyzer was applied to measure the tensile strength of HBF dough sheet, and the content of glutenin macropolymer (GMP), free sulfhydryl (-SH) and secondary structure of protein and microstructure in HBF dough sheet were investigated. Furthermore, correlations between these parameters were determined by regression analysis and Pearson correlation coefficient.
  • It was suggested that the reconstituted flours with a higher gluten index showed a higher farinograph quality number (FQN) and greater maximum resistance to extension (Rm ). HBF dough sheets with higher gluten index possessed higher GMP and lower free -SH contents, a more ordered secondary structure of protein, resulting in a more compact gluten network and a stronger tensile strength (TS).
  • The regression and correlation analysis showed that TS was positively correlated with FQN and Rm . In addition, it was significantly correlated with the content of GMP, -SH, secondary structure of protein and gluten network. It was concluded that texture analyzer could be an alternative approach to evaluate the processing quality of HBF dough sheet. Moreover, the gluten index of flours could be used to predict the processing quality of HBF dough sheet. This article is protected by copyright. All rights reserved.

Development of an Instrument to Assess the Stability of Cementless Femoral Implants Using Vibration Analysis During Total Hip Arthroplasty

Objective: The level of primary implant fixation in cementless total hip arthroplasty is a key factor for the longevity of the implant. Vibration-based methods show promise for providing quantitative information to help surgeons monitor implant fixation intraoperatively. A thorough understanding of what is driving these changes in vibrational behavior is important for further development and improvement of these methods. Additionally, an instrument must be designed to enable surgeons to leverage these methods. This study addresses both of these issues.
Method: An augmented system approach was used to develop an instrument that improves the sensitivity of the vibrational method and enables the implementation of the necessary excitation and measurement equipment. The augmented system approach took into account the dynamics of the existing bone-implant system and its interaction with the added instrument.
Results: Two instrument designs are proposed, accompanied by a convergence-based method to determine the insertion endpoint. The modal strain energy density distribution was shown to affect the vibrational sensitivity to contact changes in certain areas.
Conclusion: The augmented system approach led to an instrument design that improved the sensitivity to changes in the proximal region of the combined bone-implant-instrument system. This fact was confirmed both in silico and in vitro. Clinical Impact: The presented method and instruments address practical intraoperative challenges and provide perspective to objectively support the surgeon’s decision-making process, which will ensure optimal patient treatment.

Validation of the PAM-13 instrument in the Hungarian general population 40 years old and above

Background: Patient activation comprises the skills, knowledge and motivation necessary for patients’ effective contribution to their care. We adapted and validated the 13-item Patient Activation Measure (PAM-13) in the ≥ 40 years old Hungarian general population.
Methods: A cross-sectional web survey was conducted among 900 respondents selected from an online panel via quota sampling. After 10 days, the survey was repeated on 100 respondents. The distribution, internal consistency, test-retest reliability, factor structure, convergent, discriminant and known-groups validity of PAM-13 were assessed according to the COSMIN guidelines.
Results: The sample comprised 779 respondents. Mean (± SD) age was 60.4 ± 10.6 years, 54% were female and 67% had chronic illness. Mean (± SD) PAM-13 score was 60.6 ± 10.0. We found good internal consistency (Cronbach alpha: 0.77), moderate test-retest reliability (ICC: 0.62; n = 75), a single-factor structure and good content validity: PAM-13 showed moderate correlation with the eHealth Literacy Scale (r = 0.40), and no correlation with age (r = 0.02), education (r = 0.04) or income (ρ = 0.04). Higher PAM-13 scores were associated with fewer lifestyle risks (p < 0.001), more frequent health information seeking (p < 0.001), participation in patient education (p = 0.018) and various online health-related behaviours. When controlling for health literacy, sociodemographic factors and health status, the association of higher PAM-13 scores with overall fewer lifestyle risks, normal body mass index, physical activity and adequate diet remained significant. Similar properties were observed in the subgroup of participants with chronic morbidity, but not in the age group 65+.
Conclusion: PAM-13 demonstrated good validity in the general population. Its properties in clinical populations and the elderly as well as responsiveness to interventions warrant further research.

SmartBlock 24 x 1.5ml Thermoblock for ThermoMixer and ThermoStat instruments

E5360000038 Scientific Laboratory Supplies EACH 675 EUR

SmartBlock 24 x 0.5ml Thermoblock for ThermoMixer and ThermoStat instruments

E5361000031 Scientific Laboratory Supplies EACH 675 EUR

SmartBlock 24 x 2.0ml Thermoblock for ThermoMixer and ThermoStat instruments

E5362000035 Scientific Laboratory Supplies EACH 689.36 EUR

SmartBlock 4 x 50ml Thermoblock for ThermoMixer and ThermoStat instruments

E5365000028 Scientific Laboratory Supplies EACH 625.2 EUR

SmartBlock 8 x 15ml Thermoblock for ThermoMixer and ThermoStat instruments

E5366000021 Scientific Laboratory Supplies EACH 689.36 EUR

TIP 300UL CONDUCTIVITY - TYPE QIAGEN AND DIAGNOSTIC INSTRUMENTS,NONSTERILE,96/2304

QT-300-CBK-R CORNING 2304/pk 282 EUR

20UL CLEAR TIPS FOR PACKARDS PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS.

PK-20-R CORNING 960/pk 302.4 EUR

200UL CLEAR TIPS FOR PACKARDS PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS.

PK-200-R CORNING 960/pk 342 EUR

50UL CLEAR TIPS FOR PACKARDS PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS.

PK-50-R CORNING 960/pk 298.8 EUR

200UL CLEAR MAXYMUM RECOVERY TIPS FOR PACKARD"S PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS

PK-200-L-R CORNING 960/pk 360 EUR

SmartBlock 12mm Thermoblock for 24 x 11-11.9mm dia tubes for ThermoMixer and ThermoStat instruments

E5364000024 Scientific Laboratory Supplies EACH 838.2 EUR

SmartBlock PCR 96 Thermoblock for 96 well PCR plates inc Lid for ThermoMixer and ThermoStat instruments

E5306000006 Scientific Laboratory Supplies EACH 838.2 EUR

SmartBlock PCR 384 Thermoblock for 384 well PCR plates inc Lid for ThermoMixer and ThermoStat instruments

E5307000000 Scientific Laboratory Supplies EACH 838.2 EUR

SmartBlock plates Thermoblock for MTP and Deepwell plates inc Lid for ThermoMixer and Thermo tat instruments

E5363000039 Scientific Laboratory Supplies EACH 838.2 EUR

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 CORNING 10/pk 639.6 EUR

Add-on Instrument

C55 DataApex - 420 EUR

AnaPrep 12 Instrument

Z1321001 Biochain 1 unit 27916.8 EUR

AGS8830-8 PCR instrument

AGS8830 Daan Gene 1 unit/set Ask for price

Nucleic Acid Instrument

Smart-32 Daan Gene 1 unit/set Ask for price

Nucleic Acid Instrument

Swift-96 Daan Gene 1 unit/set Ask for price

BioDrop Instrument Cover

SPE6052 Scientific Laboratory Supplies EACH 72 EUR

BioDrop PC Instrument Cover

SPE6054 Scientific Laboratory Supplies EACH 111.6 EUR

Evaluation of a Wearable Non-Invasive Thermometer for Monitoring Ear Canal Temperature during Physically Demanding (Outdoor) Work

Aimed at preventing heat strain, health problems, and absenteeism among workers with physically demanding occupations, a continuous, accurate, non-invasive measuring system may help such workers monitor their body (core) temperature. The aim of this study is to evaluate the accuracy and explore the usability of the wearable non-invasive Cosinuss° °Temp thermometer. Ear canal temperature was monitored in 49 workers in real-life working conditions. After individual correction, the results of the laboratory and field study revealed high correlations compared to ear canal infrared thermometry for hospital use. After performance of the real-life working tasks, this correlation was found to be moderate.
It was also observed that the ambient environmental outdoor conditions and personal protective clothing influenced the accuracy and resulted in unrealistic ear canal temperature outliers. It was found that the Cosinuss° °Temp thermometer did not result in significant interference during work. Therefore, it was concluded that, without a correction factor, the Cosinuss° °Temp thermometer is inaccurate. Nevertheless, with a correction factor, the reliability of this wearable ear canal thermometer was confirmed at rest, but not in https://biodas.org/ outdoor working conditions or while wearing a helmet or hearing protection equipment.

Mobile Health-Based Thermometer for Monitoring Wound Healing After Endovascular Therapy in Patients With Chronic Foot Ulcer: Prospective Cohort StudY

Background: Foot temperature may increase after endovascular therapy, but the relationship between foot temperature and wound healing is unclear.
Objective: This study was performed to evaluate the feasibility of a mobile health (mHealth)-based thermometer for foot temperature monitoring in patients with chronic foot ulcer before and after endovascular therapy and to determine the association between temperature change and wound healing time.
Methods: This was a prospective cohort study. Patients who had a chronic foot ulcer (>3 months) and underwent endovascular therapy between June 2019 and December 2019 were included. The participants received standard medical care and endovascular therapy for revascularization. The mHealth-based thermometer, composed of 4 temperature-sensing chips, was put on the foot before and after endovascular therapy. Data from the chips were transferred to an associated mobile phone app via Bluetooth. Wound healing time was estimated using the Kaplan-Meier method, and the associations between baseline characteristics and clinical outcomes were evaluated using a Cox proportional hazard model.
Results: A total of 163 patients with chronic foot ulcer who underwent endovascular therapy were enrolled and followed up until wound healing was complete or for 180 days. The mean foot temperature before endovascular therapy was 30.6 (SD 2.8 °C). Foot temperature increased significantly (mean 32.1 °C, SD 2.8 °C; P=.01) after the procedure. Wound healing time was significantly different in the Kaplan-Meier curves of the patient group with temperature changes ≥2 °C and the group with temperature changes ≤2 °C (log-rank P<.001). A foot temperature increase ≥2 °C after endovascular therapy was associated with increased wound healing in univariate analysis (hazard ratio [HR] 1.78, 95% CI 1.24-2.76, P=.02), and the association remained significant in multivariate analysis (HR 1.69, 95% CI 1.21-2.67, P=.03).
Conclusions: The mHealth-based thermometer was feasible and useful for foot temperature monitoring, which may provide health care professionals with a new endpoint for endovascular therapy. Foot temperature increases ≥2 °C after endovascular therapy were associated with faster wound healing in patients with chronic foot ulcer. Further studies are needed, however, to confirm these findings.

Tympanic thermometers support fast and accurate temperature monitoring in acute and alternative care

This article explores body temperature and the physiological process of thermoregulation. Normal body temperature and body temperature changes are discussed, including comorbidities associated with body temperature and signs of hyperthermia and hypothermia, and the factors that affect intraoperative temperature regulation.
The evidence base behind thermometry is discussed and is applied to contemporary clinical conditions and symptoms, including: sepsis and suspected COVID-19. After discussing clinical considerations and regulations that encompass thermometry, three case studies present the use of the Genius 3 Tympanic Thermometer in clinical practice, with user feedback supporting its benefits, which include speed, accuracy and ease of use.

Mitochondria-Anchored Molecular Thermometer Quantitatively Monitoring Cellular Inflammations

Temperature in mitochondria can be a critical indicator of cell metabolism. Given the highly dynamic and inhomogeneous nature of mitochondria, it remains a big challenge to quantitatively monitor the local temperature changes during different cellular processes. To implement this task, we extend our strategy on mitochondria-anchored thermometers from “on-off” probe Mito-TEM to a ratiometric probe Mito-TEM 2.0 based on the Förster resonance energy transfer mechanism. Mito-TEM 2.0 exhibits not only a sensitive response to temperature through the ratiometric changes of dual emissions but also the specific immobilization in mitochondria via covalent bonds.
Both characters support accurate and reliable detection of local temperature for a long time, even in malfunctioning mitochondria. By applying Mito-TEM 2.0 in fluorescence ratiometric imaging of cells and zebrafishes, we make a breakthrough in the quantitative visualization of mitochondrial temperature rises in different inflammation states.

Non-invasive and wearable thermometer for continuous monitoring of core body temperature under various convective conditions

We describe the design of a thermometer that can be worn during everyday activities for monitoring core body temperature (CBT) at the skin surface. This sensor estimates the CBT by measuring the heat flux from the body core based on a thermal conductive model. The heat flux is usually affected by the ambient convective conditions (e.g. air conditioner or posture), which in turn affects the model’s accuracy. Thus, we analytically investigated heat conduction and designed a sensor interface that would be robust to convection changes. We performed an in vitro experiment and a preliminary in vivo experiment. The accuracy of CBT in an in vitro experiments was 0.1°C for convective values ranging from 0 to 1.2 m/s. The wearable thermometer has high potential as non-invasive CBT monitor.

Effect of monitoring the onset of calving by a calving alarm thermometer on the prevalence of dystocia, stillbirth, retained fetal membranes and clinical metritis in a Hungarian dairy farm

The objective of the present study was to assess the effectiveness of an intravaginal thermometer in the field prediction of the second stage of labor and to determine its impact on the health of dams and newborn calves. Holstein cows (n = 241) were randomly selected about 5 (mean ± SD: 4.7 ± 2.0) days before the expected date of calving and the thermometer was inserted into the vagina. Another 113 cattle served as controls. There was no false alarm during the experiment. The risk of dystocia (Score >1) was 1.9 times higher, the prevalence of stillbirth was 19.8 times higher, the risk of retained fetal membranes (RFM) was 2.8 times higher and the risk of clinical metritis was 10.5 times higher in the control group than in the experimental group.
The prevalence of stillbirth was 7 times higher in cows with dystocia compared to cows with eutocia. The presence of dystocia and stillbirth increased the risk of RFM 4 and 5 times, respectively. The occurrence of RFM increased the risk of development of clinical metritis with a 22 times higher odds. The results indicate that the use of calving alert systems not only facilitates controlling the time of parturition and providing prompt and appropriate calving assistance but also decreases the number of dystocia cases and improves reproductive efficiency, postpartum health of the dam and newborn calf survival.

SOM02.0 Selected Ion Monitoring 2 Components

CLPS-SOM-ISB Scientific Laboratory Supplies 1ML 118.8 EUR

Thermometer (TDC 150)

THE1802 Scientific Laboratory Supplies EACH 49.35 EUR

scanning thermometer

T8710 Consort ea 1538.4 EUR

scanning thermometer

T8720 Consort ea 1538.4 EUR

Thermometer/hygrometer

DD98463 Scientific Laboratory Supplies EACH 26.4 EUR

Thermometer -1 to 101C (0.2)

THE1372 Scientific Laboratory Supplies EACH 25.09 EUR

Thermometer -1 to 101C (0.2)

THE1374 Scientific Laboratory Supplies EACH 25.09 EUR

Testo Thermometer 110+Battery

THE2070 Scientific Laboratory Supplies EACH 144.92 EUR

Teflon Thermometer -20 to 110

THE1164 Scientific Laboratory Supplies EACH 21.96 EUR

Teflon Thermometer -10 to 210

THE1166 Scientific Laboratory Supplies EACH 26.76 EUR

Teflon Thermometer -20 to 150

THE1178 Scientific Laboratory Supplies EACH 23.54 EUR

Alarm Thermometer -50 to +150C

THE1502 Scientific Laboratory Supplies EACH 34.8 EUR

Core Thermometer (TFX410)

THE1816 Scientific Laboratory Supplies EACH 309.43 EUR

Spirit Thermometer -10 to 110C

THE1062 Scientific Laboratory Supplies EACH 4.73 EUR

Spirit Thermometer -10 to 150C

THE1064 Scientific Laboratory Supplies EACH 3.92 EUR

Spirit Thermometer -0 to 240F

THE1066 Scientific Laboratory Supplies EACH 4.73 EUR

Spirit Thermometer -10 to 110C

THE1080 Scientific Laboratory Supplies EACH 4.73 EUR

Digital Thermometer -40 to 240

THE1174 Scientific Laboratory Supplies EACH 31.46 EUR

Digital Thermometer -50 to 300

THE1176 Scientific Laboratory Supplies EACH 59.67 EUR

Testo Thermometer 925 K Type

THE2090 Scientific Laboratory Supplies EACH 130.56 EUR

H-B Durac Thermometer

BAB602150000-1EA Scientific Laboratory Supplies EACH 186 EUR

Core Thermometer (TTX 110)

THE1810 Scientific Laboratory Supplies EACH 77.03 EUR

Testo Stick Thermometer 905 T1

THE2020 Scientific Laboratory Supplies EACH 91.39 EUR

Folding thermometer white

THE4532 Scientific Laboratory Supplies EACH 61.36 EUR

Scalable and Robust Bacterial Cellulose Carbon Aerogels as Reusable Absorbents for High-Efficiency Oil/Water Separation

Efficient selective separation of oils or organic pollutants from water is important for ecological, environmental conservation and sustainable development. Various absorption methods have emerged; the majority of them still suffer from defects including low removal efficiency, a complicated preparation process, and high cost. Herein, we present a highly porous and mechanical resilient bacterial cellulose (BC) carbon aerogel directly from BC hydrogel via facile directional freeze-drying and high-temperature carbonization. The resultant BC carbon aerogel showed excellent mechanical compressibility (maximal height compression ∼99.5%) and elastic recovery due to the porous structure. Taking advantages of the high thermal stability and superhydrophobicity, the BC carbon aerogel was directly used as a versatile adsorbent for oil/water separation.
The result demonstrated that the BC carbon aerogel showed super oil/water separation selectivity with the oil absorption capacity as high as 132-274 g g-1. More importantly, the BC carbon aerogel adsorbent can be reused by a simple absorption/combustion method and still keep high-efficiency oil absorption capacity and excellent superhydrophobicity after 20 absorption/combustion cycles, displaying recyclability and robust stability. In sum, the BC carbon aerogel introduced here is easy to fabricate, ecofriendly, highly scalable, low cost, mechanically robust, and reusable; https://biodas.org/ all of these features make it highly attractive for oil/water separation application.

A camphene-camphor-polymer composite material for the production of superhydrophobic absorbent microporous foams

In a recently published paper (doi.org/10.3390/molecules26113116) on self-propelled motion of objects on the water surface, we described a novel surface-active plastic material obtained by dissolution of camphor and polypropylene in camphene at 250 [Formula: see text]C. The material has wax-like mechanical properties, can be easily formed to any moldable shape, and allows for longer and more stable self-propelled motion if compared with pure camphor or pure camphene or of a camphene-camphor wax.
Here we use scanning electron microscopy to visualize and characterize the microporous structure of the solid polypropylene foam formed in the plastic for different polypropylene contents. The topology of foams remaining in the material after camphor and camphene molecules have been removed through evaporation or dissolution is similar to polypropylene foams obtained using thermally-induced phase separation. We show that the foams have a superhydrophobic surface but strongly absorb non-polar liquids, and suggest an array of potential scientific and industrial applications.

Development of pH-responsive absorbent pad based on polyvinyl alcohol/agarose/anthocyanins for meat packaging and freshness indication

Absorbent pads with antioxidant and pH-responsive color changing functions have been developed based on polyvinyl alcohol (PVA), agarose (AG), and purple sweet potato anthocyanins (PSPA), aiming for fresh keeping and freshness indication of fresh meat. The effects of PSPA content on the structure, physical properties, and colorimetric response towards pH changing of pads were evaluated. The results showed that PSPA interacted with PVA and AG and influenced the crystallinity, thermal stability and micro-morphology of pads.
The increase of the PSPA content from 3% to 12% improved the strength and DPPH radical scavenging activity of the pads, but reduced the swelling ratio. Significant color change of the pads was observed when pH increased from 3 to 10, and the pad containing 9% PSPA presented the most distinguishable color change with the change of pH. When applied as an absorbent pad for minced meat packaging, the pad indicated the real-time spoilage of the meat through obvious color change, and also extended the shelf life by at least 24 h. Therefore, the dual-functional pad shows great potential to be applied as a smart and active packaging for fresh meat, which would play an important role in ensuring food safety and improving food storage quality.

Occurrence and distribution of organic ultraviolet absorbents in sediments from small urban rivers, Tianjin, China: Implications for risk management

Organic ultraviolet absorbents (OUVAs) in the environment have been of increasing concern because of their potential hazards. However, the OUVAs in waters is far from being well studied and little is known about their occurrence in small urban rivers. This study investigated the concentrations and distribution of eleven OUVAs in the sediments from five small urban rivers of Tianjin, China, and found total concentrations in the range of 11.6-189 ng/g dry weight. Relative to other rivers and lakes, no high concentrations of sediment OUVAs were observed in the small rivers. Benzophenone, homosalate and octocrylene were the dominant OUVAs, representing medians of 13.3%, 12.4% and 12.3% of the total concentrations, respectively.
Our observed composition profiles of these chemicals were different from those found in most of other waters. The sediment OUVAs may originate more from industrial activities than the use of cosmetics and personal care products in this area. The risk to aquatic organisms from exposure to the sediment OUVAs in these small urban rivers was considered low, except for benzophenone. However, more researches are needed to investigate the pollution and associated risks of these chemicals in urban rivers due to the complexity of their toxicity to aquatic organisms.

Mixture Compound Fertilizer and Super Absorbent Polymer Application Significantly Promoted Growth and Increased Nutrient Levels in Pinus massoniana Seedlings and Soil in Seriously Eroded Degradation Region of Southern China

  • Pinus massoniana is the pioneer tree species in the red soil regions of southern China, however, the serious understory soil erosion and nutrient deficiency in that region are the main factors restricting the growth of P. massoniana. This field study examined the effects of compound fertilizer and super absorbent polymer (SAP) on the physiology, growth characteristics, biomass, soil nutrient, plant nutrient content, and nutrient uptake efficiency of 1-year-old P. massoniana seedlings for 2 years at Changting, Fujian in South China. One control (no fertilizer, CK) and fertilization treatments were established, namely, single compound fertilizer application (0.94, 1.89, and 3.56 g⋅plant-1) and mixture compound fertilizer and SAP application (0.94 + 1.01, 1.89 + 1.01, and 3.56 + 1.01 g⋅plant-1).
  • Fertilization significantly improved the physiological performance, root collar diameter growth, height growth, biomass, and nutrient uptake of the seedlings. Compared with other fertilization treatments, the mixture compound fertilizer and SAP application significantly improved the seedling photosynthesis, which meant that the SAP had a significant effect on promoting photosynthesis. Under the mixture compound fertilizer and SAP application, the whole biomass of the seedlings was higher than that of all other treatments. Fertilization significantly increased the nitrogen (N), phosphorus (P), and potassium (K) content in the soils, leaves, stems, and roots of the seedlings, respectively.
  • The P content was the main factor affecting growth characteristics and contributed to 58.03% of the total variation in seedling growth characteristics (P < 0.01). The N:P ratio of CK in the soils, leaves, and stems were higher than that of all the fertilization treatments, indicating that the severely eroded and degraded region had little P and required much of P. The principal component analysis indicated that the F2S (1.89 + 1.01 g) was the optimum fertilization amount and method in this experiment. These results provide a theoretical basis for the fertilization management of P. massoniana forests with severely eroded and degraded red soil regions.

Absorbent paper roll

DD30640 Scientific Laboratory Supplies EACH 25.2 EUR

Cotton Wool White Absorbent 500g

COT1000 Scientific Laboratory Supplies EACH 28.46 EUR

Human anti-Mouse Antibody Absorbent (HAMA)

HAMA Alpha Diagnostics 1 gram 343.2 EUR

Chemical Absorbent Pads 50cm x 40cm Yellow

SAF3730 Scientific Laboratory Supplies PK100 121.42 EUR

Maintenance Absorbent Roll 50cm x 40m Grey

SAF3750 Scientific Laboratory Supplies EACH 120.12 EUR

Absorbent Chemical Spill Response Kit 15L Clip-Top Bag

SAF3722 Scientific Laboratory Supplies EACH 54.05 EUR

Absorbent Chemical Spill Response Kit 20L Clip-Top Bag

SAF3724 Scientific Laboratory Supplies EACH 56.92 EUR

RF Absorbent for the removal of IgG in human plasma/serum

RF-ABS Alpha Diagnostics 100 tests 270 EUR

Absorbent Chemical Spill Response Kit 80L Circular Static Bin

SAF3716 Scientific Laboratory Supplies EACH 157.98 EUR

Cryo Express Dry Shipper with replaceable absorbent Material (CXR100)

TW-CXR100 MiTeGen 1 SHIPPER 1365 EUR

S. Pneumococcal CWPS/22F Absorbent solution for removing/adsorbing non-specific CWPS/22F from human or animal samples (sufficient for 50 samples)

560-CW-ABS Alpha Diagnostics 1 vial 416.4 EUR

100ml absorbency pad

SCIEA08 Scientific Laboratory Supplies PK500 117.42 EUR

A6 95kPa Specimen Transport Pouch with 20mL Absorbency Pad

COV1138 Scientific Laboratory Supplies PK1000 1279.49 EUR

A5 Specimen bag with Document Pouch Inserted 20ml absorbency

COV1147 Scientific Laboratory Supplies PK500 378.62 EUR

450ML Carbamate-1 CO2 Absorber

NAT1386 Scientific Laboratory Supplies 450ML 91.2 EUR

Goat anti-Mouse IgG(H+L) Cross absorbed against human immunoglobulins

E16SPU1011-100 EnoGene 100µg 286.8 EUR

Goat anti-Mouse IgG(H+L) Cross absorbed against human immunoglobulins

E16SPU1011-1000 EnoGene 1000µg 520.8 EUR

Goat anti-Mouse IgG(H+L) Cross absorbed against human immunoglobulins

E16SPU1011-500 EnoGene 500µg 411.6 EUR

Goat anti-Rabbit IgG(H+L) Cross absorbed against human immunoglobulins

E16SPU1021-100 EnoGene 100µg 286.8 EUR

Goat anti-Rabbit IgG(H+L) Cross absorbed against human immunoglobulins

E16SPU1021-1000 EnoGene 1000µg 520.8 EUR

Goat anti-Rabbit IgG(H+L) Cross absorbed against human immunoglobulins

E16SPU1021-500 EnoGene 500µg 411.6 EUR

Goat anti-Human IgG(H+L) Cross absorbed against mouse immunoglobulins

E16SPU1031-100 EnoGene 100µg 286.8 EUR

Superhydrophobic paper in the development of disposable labware and lab-on-paper devices

Traditionally in superhydrophobic surfaces history, the focus has frequently settled on the use of complex processing methodologies using nonbiodegradable and costly materials. In light of recent events on lab-on-paper emergence, there are now some efforts for the production of superhydrophobic paper but still with little development and confined to the fabrication of flat devices. This work gives a new look at the range of possible applications of bioinspired superhydrophobic paper-based substrates, obtained using a straightforward surface modification with poly(hydroxybutyrate). As an end-of-proof of the possibility to create lab-on-chip portable devices, the patterning of superhydrophobic paper with different wettable shapes is shown with low-cost approaches.
Furthermore, we suggest the use of superhydrophobic paper as an extremely low-cost material to design essential nonplanar lab apparatus, including reservoirs for liquid storage and manipulation, funnels, tips for pipettes, or accordion-shaped substrates for liquid transport or mixing. Such devices take the advantage of the self-cleaning and extremely water resistance properties of the surfaces https://biodas.org/ as well as the actions that may be done with paper such as cut, glue, write, fold, warp, or burn. The obtained substrates showed lower propensity to adsorb proteins than the original paper, kept superhydrophobic character upon ethylene oxide sterilization and are disposable, suggesting that the developing devices could be especially adequate for use in contact with biological and hazardous materials.

Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents

Zinc is an allosteric modulator of glycine receptor function, enhancing the effects of glycine at nM to low μM concentrations, and inhibiting its effects at higher concentrations. Because of zinc’s high potency at the glycine receptor, there exists a possibility that effects attributed solely to exogenously-applied glycine in fact contain an undetected contribution of zinc acting as an allosteric modulator. We found that glycine solutions made up in standard buffers and using deionized distilled water produced effects that could be decreased by the zinc chelator tricine.
This phenomenon was observed in three different vials tested and persisted even if vials were extensively washed, suggesting the zinc was probably present in the buffer constituents. In addition, polystyrene, but not glass, pipets bore a contaminant that enhanced glycine receptor function and that could also be antagonized by tricine. Our findings suggest that without checking for this effect using a chelator such as tricine, one cannot assume that responses elicited by glycine applied alone are not necessarily also partially due to some level of allosteric modulation by zinc.

Labware additives identified to be selective monoamine oxidase-B inhibitors

Plastic labware is used in all processes of modern pharmaceutical research, including compound storage and biological assays. The use of these plastics has created vast increases in productivity and cost savings as experiments moved from glass test tubes and capillary pipettes to plastic microplates and multichannel liquid handlers. One consequence of the use of plastic labware, however, is the potential release of contaminants and their resultant effects on biological assays.
We report herein the identification of biologically active substances released from a commonly used plastic microplate. The active contaminants were identified by gas chromatography-mass spectroscopy as dodecan-1-ol, dodecyl 3-(3-dodecoxy-3-oxopropyl)sulfanylpropanoate, and dodecanoic acid, and they were found to be selective monoamine oxidase-B inhibitors.

Open Labware: 3-D printing your own lab equipment

The introduction of affordable, consumer-oriented 3-D printers is a milestone in the current “maker movement,” which has been heralded as the next industrial revolution. Combined with free and open sharing of detailed design blueprints and accessible development tools, rapid prototypes of complex products can now be assembled in one’s own garage–a game-changer reminiscent of the early days of personal computing. At the same time, 3-D printing has also allowed the scientific and engineering community to build the “little things” that help a lab get up and running much faster and easier than ever before.

3D Printing in the Laboratory: Maximize Time and Funds with Customized and Open-Source Labware

3D-Printed Labware for High-Throughput Immobilization of Enzymes

  1. In continuous flow biocatalysis, chemical transformations can occur under milder, greener, more scalable, and safer conditions than conventional organic synthesis. However, the method typically involves extensive screening to optimize each enzyme’s immobilization on its solid support material. The task of weighing solids for large numbers of experiments poses a bottleneck for screening enzyme immobilization conditions.
  2. For example, screening conditions often require multiple replicates exploring different support chemistries, buffer compositions, and temperatures. Thus, we report 3D-printed labware designed to measure and handle solids in multichannel format and expedite screening of enzyme immobilization conditions.
  3. To demonstrate the generality of these advances, alkaline phosphatase, glucose dehydrogenase, and laccase were screened for immobilization efficiency on seven resins. The results illustrate the requirements for optimization of each enzyme’s loading and resin choice for optimal catalytic performance. Here, 3D-printed labware can decrease the requirements for an experimentalist’s time by >95%.
  4. The approach to rapid optimization of enzyme immobilization is applicable to any enzyme and many solid support resins. Furthermore, the reported devices deliver precise and accurate aliquots of essentially any granular solid material.

Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research

Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials’ walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable.
The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 108 PFU/ml to around 1010 PFU/ml from the suspension. The adsorption onto the container’s wall might result in complete scavenging of virions from the bulk. We developed two methods to overcome this issue. The addition of surfactant Tween20 and/or plasma treatment provides a remedy by modulating surface wettability and inhibiting virions’ adsorption. Plastic containers are essential consumables in the daily use of many bio-laboratories. Thus, this is important not only for phage-related research (e.g., the use of phage therapies as an alternative for antibiotics) but also for data comparison and reproducibility in the field of biochemistry and virology.

Benchmark Agarose LE, 25g

A1700 Benchmark Scientific 1 PC 56.3 EUR

Benchmark Agarose LE, 100g

A1701 Benchmark Scientific 1 PC 135.8 EUR

Benchmark Agarose LE, 500g

A1705 Benchmark Scientific 1 PC 473.2 EUR

Benchmark Agarose 3:1, 100g

A1801-31 Benchmark Scientific 1 PC 287 EUR

Benchmark Agarose LM, Low Melt, 100g

A1801-LM Benchmark Scientific 1 PC 377.1 EUR

Benchmark Agarose HR, PCR Grade for DNA fragments between 20 to 800bp, 100g

A1801-HR Benchmark Scientific 1 PC 326.1 EUR

Benchmark Printer 230V

AUT2743 Scientific Laboratory Supplies EACH 488.29 EUR

Benchmark Digital Hotplate 230V

MIX1262 Scientific Laboratory Supplies EACH 416.4 EUR

Benchmark Orbi-Shaker CO2 230V

MIX7260 Scientific Laboratory Supplies EACH 5173.2 EUR

Benchmark Hotplate 17.8cm x 17.8cm 230V

MIX1301 Scientific Laboratory Supplies EACH 390 EUR

Benchmark Replacement Cap Blue 50mL

BOT1910 Scientific Laboratory Supplies PK10 46.8 EUR

Benchmark Refill Glass Beads 3mm 1000g

STE1042 Scientific Laboratory Supplies EACH 42.12 EUR

Benchmark Digital Magnetic Stirrer 230V

MIX1263 Scientific Laboratory Supplies EACH 416.4 EUR

Benchmark MiniMag Magnetic Stirrer 240V

MIX1290 Scientific Laboratory Supplies EACH 177.6 EUR

Benchmark MyFuge 5 MicroCentrifuge 230V

CEN1870 Scientific Laboratory Supplies EACH 700.8 EUR

Benchmark Replacement Sealing Ring 50mL

BOT1911 Scientific Laboratory Supplies PK10 20.4 EUR

Benchmark Magnetic Stirrer 17.8cm x 17.8cm 230V

MIX1302 Scientific Laboratory Supplies EACH 390 EUR

Benchmark StripSpin 12 Mini Centrifuge 230V

CEN1714 Scientific Laboratory Supplies EACH 507.6 EUR

Benchmark Replacement Sealing Ring 100-2000mL

BOT1909 Scientific Laboratory Supplies PK10 20.4 EUR

Benchmark StripSpin Round Rotor 8 position

CEN1715 Scientific Laboratory Supplies EACH 78 EUR

Benchmark 8 x 5mL Rotor for MC-24 Touch

CEN1850 Scientific Laboratory Supplies EACH 1441.2 EUR

Benchmark BenchMasher Blender Bag 400mL x 60um 300 x 180mm

HOM3102 Scientific Laboratory Supplies PK500 118.8 EUR

Benchmark hybex Replacement Cap Blue 100-2000mL

BOT1867 Scientific Laboratory Supplies PK10 52.62 EUR

Benchmark High Temperature Cap Red (up to 180C)

BOT1908 Scientific Laboratory Supplies PK10 199.2 EUR

Contaminating levels of zinc found in commonly-used labware and buffers affect glycine receptor currents

Zinc is an allosteric modulator of glycine receptor function, enhancing the effects of glycine at nM to low μM concentrations, and inhibiting its effects at higher concentrations. Because of zinc’s high potency at the glycine receptor, there exists a possibility that effects attributed solely to exogenously-applied glycine in fact contain an undetected contribution of zinc acting as an allosteric modulator. We found that glycine solutions made up in standard buffers and using deionized distilled water produced effects that could be decreased by the zinc chelator tricine.
This phenomenon was observed in three different vials tested and persisted even if vials were extensively washed, suggesting the zinc was probably present in the buffer constituents. In addition, polystyrene, but not glass, pipets bore a contaminant that enhanced glycine receptor function and that could also be antagonized by tricine. Our findings suggest that without checking for this effect using a chelator such as tricine, one cannot assume that responses elicited by glycine applied alone are not necessarily also partially due to some level of allosteric modulation by zinc.

Superhydrophobic paper in the development of disposable labware and lab-on-paper devices

Traditionally in superhydrophobic surfaces history, the focus has frequently settled on the use of complex processing methodologies using nonbiodegradable and costly materials. In light of recent events on lab-on-paper emergence, there are now some efforts for the production of superhydrophobic paper https://biodas.org/ but still with little development and confined to the fabrication of flat devices. This work gives a new look at the range of possible applications of bioinspired superhydrophobic paper-based substrates, obtained using a straightforward surface modification with poly(hydroxybutyrate). As an end-of-proof of the possibility to create lab-on-chip portable devices, the patterning of superhydrophobic paper with different wettable shapes is shown with low-cost approaches.
Furthermore, we suggest the use of superhydrophobic paper as an extremely low-cost material to design essential nonplanar lab apparatus, including reservoirs for liquid storage and manipulation, funnels, tips for pipettes, or accordion-shaped substrates for liquid transport or mixing. Such devices take the advantage of the self-cleaning and extremely water resistance properties of the surfaces as well as the actions that may be done with paper such as cut, glue, write, fold, warp, or burn. The obtained substrates showed lower propensity to adsorb proteins than the original paper, kept superhydrophobic character upon ethylene oxide sterilization and are disposable, suggesting that the developing devices could be especially adequate for use in contact with biological and hazardous materials.

3D Printing in the Laboratory: Maximize Time and Funds with Customized and Open-Source Labware

3D-Printed Labware for High-Throughput Immobilization of Enzymes

In continuous flow biocatalysis, chemical transformations can occur under milder, greener, more scalable, and safer conditions than conventional organic synthesis. However, the method typically involves extensive screening to optimize each enzyme’s immobilization on its solid support material. The task of weighing solids for large numbers of experiments poses a bottleneck for screening enzyme immobilization conditions. For example, screening conditions often require multiple replicates exploring different support chemistries, buffer compositions, and temperatures.
Thus, we report 3D-printed labware designed to measure and handle solids in multichannel format and expedite screening of enzyme immobilization conditions. To demonstrate the generality of these advances, alkaline phosphatase, glucose dehydrogenase, and laccase were screened for immobilization efficiency on seven resins. The results illustrate the requirements for optimization of each enzyme’s loading and resin choice for optimal catalytic performance. Here, 3D-printed labware can decrease the requirements for an experimentalist’s time by >95%. The approach to rapid optimization of enzyme immobilization is applicable to any enzyme and many solid support resins. Furthermore, the reported devices deliver precise and accurate aliquots of essentially any granular solid material.

Additive manufactured customizable labware for biotechnological purposes

An Economical, Portable Manual Cryogenic Plunge Freezer for the Preparation of Vitrified Biological Samples for Cryogenic Electron Microscopy.

Visualizing biological structures and cellular processes in their native state is a major goal of many scientific laboratories. In the past 20 years, the technique of preserving samples by vitrification has greatly expanded, specifically for use in cryogenic electron microscopy (cryo-EM). Here, we report on improvements in the design and use of a portable manual cryogenic plunge freezer that is intended for use in laboratories that are not equipped for the cryopreservation of samples.

The construction of the instrument is economical, can be produced by a local machine shop without specialized equipment, and lowers the entry barriers for newcomers with a reliable alternative to costly commercial equipment. The improved design allows for successful freezing of isolated proteins for single particle analysis https://biodas.org/ as well as bacterial cells for cryo-electron tomography. With this instrument, groups will be able to prepare vitreous samples whenever and wherever necessary, which can then be imaged at local or national cryo-EM facilities.

Successful short-term cryopreservation of volume-reduced cord blood units in a cryogenic mechanical freezer: effects on cell recovery, viability, and clonogenic potential

BACKGROUND
Cord blood (CB) units are stored from weeks to years in liquid- or vapor-phase nitrogen until they are used for transplantation. We examined the effects of cryostorage in a mechanical freezer at -150°C on critical quality control variables of CB collections to investigate the possible use of mechanical freezers at -150°C as an alternative to storage in liquid- (or vapor-) phase nitrogen.
METHODS
A total of 105 CB units were thawed and washed at different time intervals (6, 12, 24, and 36 months). For every thawed CB unit, samples were removed and cell enumeration (total nucleated cells [TNCs], mononuclear cells [MNCs], CD34+, CD133+) was performed. In addition, viability was obtained with the use of flow cytometry, and recoveries were calculated. Also, total absolute colony-forming unit counts were performed and progenitor cell recoveries were studied by clonogenic assays.
RESULTS
Significant differences (p < 0.05) were observed in certain variables (TNCs, MNC numbers, viability) when they were examined in relation with time intervals, while others (CD34+, CD133+) were relatively insensitive (p = NS) to the duration of time interval the CB units were kept in cryostorage condition.
CONCLUSIONS
The data presented suggest that cryopreservation of CB units in a mechanical freezer at -150°C may represent an alternative cryostorage condition for CB cryopreservation.

Realignment-free cryogenic macroscopic optical cavity coupled to an optical fiber

We present a cryogenic setup where an optical Fabry-Perot resonator is coupled to a single-mode optical fiber with coupling efficiency above 90% at mK temperatures without realignment during cooling down. The setup is prealigned at room temperature to compensate for the thermal contraction and change of the refractive index of the optical components during cooling down.

The high coupling efficiency is achieved by keeping the setup rotation-symmetric around the optical axis. The majority of the setup components are made of Invar (FeNi36), which minimizes the thermal contraction. High coupling efficiency is essential in quantum optomechanical experiments.

Extraordinary approach to further boost plasmonic NIR-SERS by cryogenic temperature-suppressed non-radiative recombination

We report an effective strategy to promote the near-infrared surface-enhanced Raman scattering spectroscopy (NIR-SERS) activity by boosting the photon-induced charge transfer (PICT) efficiency at cryogenic temperature. Based on as-prepared Au/Ag nano-urchins (NUs) with abundant surface defects, the extremely low temperature (77 K) can significantly weaken the metallic lattice vibration and reduce the recombination of thermal phonons and photoexcited electrons, then accelerate the migration of energetic electrons.
It enables the NIR-SERS detection limit of dye molecules to be achieved at 10-17 M, which is nearly three orders of magnitude better than that at room temperature. The present work provides a new, to the best of our knowledge, approach for ultra-trace NIR-SERS bioanalysis.

Ultra-stretchable and fast self-healing ionic hydrogel in cryogenic environments for artificial nerve fiber

Self-healing materials behave irreplaceable advantages in biomimetic intelligent robots (BIR) for avoiding or reducing safety hazards and economic losses from accidental damage during service. However, the self-healing ability is unreservedly lost and even becomes rigid, fragile in the cryogenic environment where BIR is precisely needed. Here, we report a versatile ionic hydrogel with fast self-healing ability, ultra-stretchability, and stable conductivity, even at -80℃.
The hydrogel is systematically optimized to improve hydrogen-bonded network nanostructure, coordinated achieving a quick self-healing ability within 10 min, large deformation tolerance of over 7000%, superior conductivity of 11.76 S·cm-1 and anti-freezing ability, which is difficult to obtain simultaneously. Such hydrogel provides new opportunities for artificial electronic devices in harsh environments. As a prospective application, we fabricate an artificial nerve fiber by mimicking the structure and functions of the myelinated axon, exhibiting the property of fast and potential-gated signal transmission.
This artificial nerve fiber is integrated into a robot for demonstrating a real-time high fidelity and high throughput information interaction under big deformation and cryogenic temperature. The hydrogel and bionic device will bring pioneering functions for robots and open a broad application scenario in extreme conditions. This article is protected by copyright. All rights reserved.

Cryogenic temperature sensing based on the temperature dependence of color centers in optical fibers

A cryogenic temperature sensor based on the temperature dependence of stable color centers in a commercial single-mode optical fiber is proposed. The radiation induced attenuation spectra at different temperatures are measured and decomposed by Ge-NBOHC and Ge(X) color centers. The configurational coordinate model is used to explain the temperature properties of the color centers.
A series of experiments are conducted to evaluate its performance in the temperature range from 10°C to -196°C, and the results suggest that the temperature sensitivity is ∼0.17 dB/km/°C with a resolution of 0.034°C, and the nonlinearity and repeatability error are ±3.8% and 1.4%, respectively.

Revealing the Intrinsic Atomic Structure and Chemistry of Amorphous LiO 2-Containing Products in Li-O 2 Batteries Using Cryogenic Electron Microscopy

Aprotic lithium-oxygen batteries (LOBs) are promising energy storage systems characterized by ultrahigh theoretical energy density. Extensive research has been devoted to this battery technology, yet the detailed operational mechanisms involved, particularly unambiguous identification of various discharge products and their specific distributions, are still unknown or are subjects of controversy. This is partly because of the intrinsic complexity of the battery chemistry but also because of the lack of atomic-level insight into the oxygen electrodes acquired via reliable techniques. In the current study, it is demonstrated that electron beam irradiation could induce crystallization of amorphous discharge products. Cryogenic conditions and a low beam dosage have to be used for reliable transmission electron microscopy (TEM) characterization.
High-resolution cryo-TEM and electron energy loss spectroscopy (EELS) analysis of toroidal discharge particles unambiguously identified the discharge products as a dominating amorphous LiO2 phase with only a small amount of nanocrystalline Li2O2 islands dispersed in it. In addition, uniform mixing of carbon-containing byproducts is identified in the discharge particles with cryo-EELS, which leads to a slightly higher charging potential. The discharge products can be reversibly cycled, with no visible residue after full recharge. We believe that the amorphous superoxide dominating discharge particles can lead researchers to reconsider the chemistry of LOBs and pay special attention to exclude beam-induced artifacts in traditional TEM characterizations.

10K Cryogenic Freezer With CS200 Controller and Gas ByPass

TW-10K-CS200-GBP MiTeGen 1 UNIT 19442 EUR

24K Cryogenic Freezer With CS200 Controller and Gas ByPass

TW-24K-CS200-GBP MiTeGen 1 UNIT 22879 EUR

38K Cryogenic Freezer With CS200 Controller and Gas ByPass

TW-38K-CS200-GBP MiTeGen 1 UNIT 31556 EUR

80K Cryogenic Freezer With CS200 Controller and Double Step

TW-LABS80K-CS-DS MiTeGen 1 UNIT 64636 EUR

80K Cryogenic Freezer With CS200 Controller and Locking Step

TW-LABS80K-CS MiTeGen 1 UNIT 63686 EUR

10K Cryogenic Freezer No Controller

TW-10K MiTeGen 1 UNIT 14003 EUR

24K Cryogenic Freezer No Controller

TW-24K MiTeGen 1 UNIT 19318 EUR

38K Cryogenic Freezer No Controller

TW-38K MiTeGen 1 UNIT 28465 EUR

94K Cryogenic Freezer With CS200 Controller and Double Locking Steps and Fill Hose with Adapter

TW-LABS94K-SP MiTeGen 1 UNIT 71752 EUR

10K Cryogenic Freezer With CS100 Controller

TW-10K-CS100 MiTeGen 1 UNIT 17922 EUR

10K Cryogenic Freezer With CS200 Controller

TW-10K-CS200 MiTeGen 1 UNIT 18872 EUR

24K Cryogenic Freezer With CS100 Controller

TW-24K-CS100 MiTeGen 1 UNIT 21359 EUR

24K Cryogenic Freezer With CS200 Controller

TW-24K-CS200 MiTeGen 1 UNIT 22309 EUR

38K Cryogenic Freezer With CS200 Controller

TW-38K-CS200 MiTeGen 1 UNIT 30606 EUR

20K Cryogenic Freezer With CS200 Controller

TW-LABS20K-CS MiTeGen 1 UNIT 29839 EUR

40K Cryogenic Freezer With CS200 Controller

TW-LABS40K-CS MiTeGen 1 UNIT 41579 EUR

3K Cryogenic Freezer With Stainless Steel Exterior

TW-3KSBL MiTeGen 1 UNIT 3870 EUR

Special cryogenics label 38 x 19 mm white colour

DD53075 Scientific Laboratory Supplies PK1200 160.8 EUR

Special cryogenics label 33 x 13 mm assorted colours

DD53520 Scientific Laboratory Supplies PK1700 160.8 EUR

Special cryogenics label 38 x 6 mm assorted colours

DD53526 Scientific Laboratory Supplies PK3120 154.8 EUR

CO2 Back Up for ULT Freezers

SLS1072 Scientific Laboratory Supplies EACH 3034.8 EUR

COOLCELL® LX-4 PACK, 4 COLOURS, CELL FREEZING CONTAINER, FOR 12 X 1ML OR 2ML CRYOGENIC VIALS

432138 CORNING 1/pk 714 EUR

NBS CO2 Backup for Innova Freezers

FRE6110 Scientific Laboratory Supplies EACH 2235.6 EUR

NBS LN2 Backup for Innova Freezers

FRE6114 Scientific Laboratory Supplies EACH 2235.6 EUR

NBS CO2 Backup for Premium Freezers

FRE6112 Scientific Laboratory Supplies EACH 2077.2 EUR

NBS LN2 Backup for Premium Freezers

FRE6116 Scientific Laboratory Supplies EACH 2235.6 EUR

Perspectives of farmers and tourists on agricultural abandonment in east Lesvos, Greece.

Perspectives of farmers and tourists on agricultural abandonment in east Lesvos, Greece.

Multi-stakeholder perceptions of panorama modifications are more and more acknowledged as important inputs to discussions on future panorama developments, significantly when addressing the way forward for European rural areas experiencing agricultural abandonment.

This analysis presents a case exploration of abandonment of olive plantations in east Lesvos, Greece. We carried out two units of semi-structured interviews to narrate an exploration on native farmers’ capacity and willingness to keep up the plantations, to the outcomes of a panorama choice survey undertaken with vacationers. Three farmer varieties are recognized following a cluster evaluation based mostly on attributes of particular person capacity and willingness to farm. Farmers belonging to the prevalent kind revealed low capacity and willingness and count on to additional extensify their farms.

The remaining two farmer varieties have greater willingness; they’re motivated by cultural causes, extra incessantly expressing a want to keep up their land underneath household possession, and partake in social cooperative initiatives selling practices valorizing the olive plantations. We define how these varieties work together with regional drivers of change, and partly additionally contribute to persistence of abandonment by way of constrained capacity to farm.

Abandonment doesn’t align with present panorama preferences of vacationers, who favor cultivated landscapes, components of traditionality inside constructed infrastructure and undertake nature-based actions. We focus on how excessive willingness to farm related to skilled and pluri-active types of farming could nevertheless present alternatives to keep up the cultivated panorama and synergize with (agri-)tourism demand. Our findings are corresponding to these of different European research, contributing to discussions on the way forward for its rural landscapes.

Perspectives of farmers and tourists on agricultural abandonment in east Lesvos, Greece.
Views of farmers and vacationers on agricultural abandonment in east Lesvos, Greece.

Immunophenotypic characterization, multi-lineage differentiation and growing older of zebrafish coronary heart and liver tissue-derived mesenchymal stem cells as a novel strategy in stem cell-based remedy.

Mesenchymal stem cells (MSCs) are a very good mannequin for preclinical and scientific investigations, and various sources of MSCs are topic to intensive experiments. On this examine, mesenchymal stem cells (MSCs) have been remoted from coronary heart and liver tissue of Zebrafish (Danio rerio). The flow-cytometry in addition to RT-PCR have been used to investigate the expression of a panel of cell floor markers CD44, CD90, CD31 and CD34.

Rapid antibody fluorescent labeling kit (eFluor 405)

EGQ0042 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 488)

EGQ0043 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 555)

EGQ0044 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 594)

EGQ0045 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 647)

EGQ0046 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 680)

EGQ0047 1 labeing(100ug/T)
EUR 225

Rapid antibody fluorescent labeling kit (eFluor 750)

EGQ0048 1 labeing(100ug/T)
EUR 225

Paritaprevir(ABT-450)

20-abx184297
  • EUR 1496.40
  • EUR 1095.60
  • 10 mg
  • 5 mg

iFluor® 450 maleimide

1057 1 mg
EUR 222

AKT1 (Ab-450) Antibody

21502-100ul 100ul
EUR 302.4

AKT1 (Ab-450) Antibody

21502-50ul 50ul
EUR 224.4

AKT1 (Ab-450) Antibody

CSB-PA969941- each
EUR 402
Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

AKT1 (Ab-450) Antibody

CSB-PA969941-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against AKT1 (Ab-450). Recognizes AKT1 (Ab-450) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF;WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200

anti-AKT1 (Ab-450)

LF-PA20679 100 ul
EUR 400.8
Description: Rabbit polyclonal to AKT1

Nhe I unit: 450

YRNHE1 1 vial Ask for price

mFluorâ„¢ Violet 450 SE

1150-1mg 1 mg
EUR 222
Description: AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications.

mFluorâ„¢ Violet 450 acid

1140-5mg 5 mg
EUR 222
Description: AAT Bioquest's mFluorâ„¢ dyes are developed for multicolor flow cytometry-focused applications.

mFluorâ„¢ Violet 450 maleimide

1600-1mg 1 mg
EUR 222
Description: AAT Bioquest\'s mFluorâ„¢ dyes are developed for multicolor flow cytometry-focused applications.

mFluorâ„¢ Violet 450 Azide

1690-1mg 1 mg
EUR 308
Description: AAT Bioquest's mFluorâ„¢ dyes are developed for multicolor flow cytometry-focused applications.

iFluor® 450 maleimide

1057-1mg 1 mg
EUR 222
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

Akt (Ab-450) Antibody

E11-0406B 100μg
EUR 225
Description: Available in various conjugation types.

AKT1 (Ab-450) Antibody

E021502-1 50μg/50μl
EUR 100
Description: Available in various conjugation types.

AKT1 (Ab-450) Antibody

E021502-2 100μg/100μl
EUR 170
Description: Available in various conjugation types.

Polyclonal SNX4 Antibody (aa438-450)

AMM07924G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human SNX4 (aa438-450). This antibody is tested and proven to work in the following applications:

Polyclonal PACSIN2 Antibody (aa400-450)

APR17733G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human PACSIN2 (aa400-450). This antibody is tested and proven to work in the following applications:

Polyclonal CYP1B1 Antibody (aa400-450)

APG02857G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CYP1B1 (aa400-450). This antibody is tested and proven to work in the following applications:

Polyclonal TLR1 Antibody (aa400-450)

APR02824G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TLR1 (aa400-450). This antibody is tested and proven to work in the following applications:

mFluor™ Violet 450 maleimide

1600 1 mg
EUR 222

mFluor™ Violet 450 Azide

1690 1 mg
EUR 308

mFluor™ Violet 450 SE

1150 1 mg
EUR 222

mFluor™ Violet 450 acid

1140 5 mg
EUR 222

Bottle Centrifuge Pp 450 Ml

3141-0500 PK4
EUR 172.8

AKT1 (Ab-450) Conjugated Antibody

C21502 100ul
EUR 476.4

iFluor® 450 succinimidyl ester

71506 5 mg
EUR 781

iFluor® 450 succinimidyl ester

71556 10 mg
EUR 1301

iFluor® 450 succinimidyl ester

71026 100 ug
EUR 82

Magnetic Beads (DNA) 450 mL

P920-450 - Ask for price

iFluor® 450 succinimidyl ester

71026-100ug 100 ug
EUR 82
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

iFluor® 450 succinimidyl ester

71506-5mg 5 mg
EUR 781
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

iFluor® 450 succinimidyl ester

71556-10mg 10 mg
EUR 1301
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

mFluorâ„¢ Violet 450-streptavidin conjugate

16930-100ug 100 ug
EUR 109
Description: Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin.

mFluorâ„¢ Violet 450-VAD-FMK

13475-25Tests 25 Tests
EUR 109
Description: FAM-VAD is a blue fluorescent cell-permeable polycaspase inhibitor to target caspases 1, 2, 3, 6, 8, 9, or 10.

iFluor® 450 succinimidyl ester

1026-1mg 1 mg
EUR 222
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies.

TMB Stop Reagent 450 nm

21530071-1 100 mL
EUR 20.55

TMB Stop Reagent 450 nm

21530071-2 500 mL
EUR 43.56

TMB Stop Reagent 450 nm

21530071-3 1 L
EUR 63.61

Akt (Ab-450) polyclonal antibody

E43P0725 100ul
EUR 225
Description: Available in various conjugation types.

Akt (Ab-450) polyclonal antibody

E43SP1810 100ul
EUR 225
Description: Available in various conjugation types.

EFL300 Sieve Shaker

SIE3078 EACH
EUR 3378

Polyclonal CYP24 / CYP24A1 Antibody (aa400-450)

APG02862G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human CYP24 / CYP24A1 (aa400-450). This antibody is tested and proven to work in the following applications:

mFluor™ Violet 450-VAD-FMK

13475 25 Tests
EUR 109

mFluor™ Violet 450-streptavidin conjugate

16930 100 ug
EUR 109

mFluorâ„¢ Violet 450-PEG4-Biotin Conjugate

3116-1mg 1 mg
EUR 195
Description: mFluorâ„¢ Violet 450-PEG4-Biotin Conjugate contains mFluorâ„¢ Violet 450 fluorophore that is well excited by the common 405 nm violet laser.

Annexin V-mFluorâ„¢ Violet 450 conjugate

20080-100tests 100 tests
EUR 222
Description: Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

Polypropylene Magnet Retriever 450 x 10mm

STI5212 EACH
EUR 9.6

Polyclonal TUBB / Beta Tubulin Antibody (aa401-450)

APR13866G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TUBB / Beta Tubulin (aa401-450). This antibody is tested and proven to work in the following applications:

Polyclonal OLFM4 / Olfactomedin 4 Antibody (aa400-450)

APR02466G 0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human OLFM4 / Olfactomedin 4 (aa400-450). This antibody is tested and proven to work in the following applications:

Annexin V-mFluor™ Violet 450 conjugate

20080 100 tests
EUR 222

Filter for Accuris Microplate Reader, 450 nm

MR9600-450 1 PC
EUR 225.9

CytoCalceinâ„¢ Violet 450 *Excited at 405 nm*

22012-1mg 1 mg
EUR 222
Description: CytoCalcein™ Violet 450 is designed for labeling live cells in the same way to calcein, AM.

Recombinant Salmonella envZ Protein (aa 1-450)

VAng-Wyb0428-inquire inquire Ask for price
Description: Salmonella typhi Osmolarity sensor protein EnvZ, recombinant protein.

Elab Fluor® Violet 450 Labeling Kit

E-LK-E010-10Reaction 10 Reaction
EUR 1000

Elab Fluor® Violet 450 Labeling Kit

E-LK-E010-1Reaction 1 Reaction
EUR 260

Elab Fluor® Violet 450 Labeling Kit

E-LK-E010-3Reaction 3 Reaction
EUR 500

EFL1 Antibody / EFTUD1

F54540-0.08ML 0.08 ml
EUR 140.25
Description: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]

EFL1 Antibody / EFTUD1

F54540-0.4ML 0.4 ml
EUR 322.15
Description: Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits. [UniProt]

Monoclonal TUBB3 / Tubulin Beta 3 Antibody (aa436-450)

APR13878G 0.05mg
EUR 580.8
Description: A Monoclonal antibody against Human TUBB3 / Tubulin Beta 3 (aa436-450). The antibodies are raised in Mouse. This antibody is applicable in WB and IHC-P, ICC

Polyclonal HTR2C / 5-HT2C Receptor Antibody (aa400-450)

APR07860G 0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human HTR2C / 5-HT2C Receptor (aa400-450). This antibody is tested and proven to work in the following applications:

iFluor® 450 Anti-human CD3 Antibody *HIT3a*

10030040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *HIT3a*

10030041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *HIT3b*

10031040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *HIT3b*

10031041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *UCHT1*

10032040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *UCHT1*

10032041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *SK7*

10033040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD3 Antibody *SK7*

10033041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *HIT4a*

10040040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *HIT4a*

10040041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *SK3*

10042040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD4 Antibody *SK3*

10042041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *HISM2*

10050040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *HISM2*

10050041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *L17F12*

10051040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *L17F12*

10051041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *ICRF44*

10112040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *ICRF44*

10112041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11c Antibody *3.9*

10113040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11c Antibody *3.9*

10113041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD13 Antibody *WM15*

10130040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD13 Antibody *WM15*

10130041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD14 Antibody *61D3*

10141040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD14 Antibody *61D3*

10141041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *HI149*

10010040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *HI149*

10010041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *SN13*

10012040 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *SN13*

10012041 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *L161*

10013040 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD1 Antibody *L161*

10013041 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD2 Antibody *HIT11*

10020040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD2 Antibody *HIT11*

10020041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD21 Antibody *HI21a*

10210040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD21 Antibody *HI21a*

10210041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD22 Antibody *HIB22*

10220040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD22 Antibody *HIB22*

10220041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD24 Antibody *HI45*

10240040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD24 Antibody *HI45*

10240041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *HI25a*

10250040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *HI25a*

10250041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *7G7B6*

10251040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD25 Antibody *7G7B6*

10251041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD27 Antibody *LT27*

10270040 100 tests
EUR 547
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD27 Antibody *LT27*

10270041 500 tests
EUR 2051
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD9 Antibody *HI9a*

10090040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD9 Antibody *HI9a*

10090041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD10 Antibody *HI10a*

10100040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD10 Antibody *HI10a*

10100041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11a Antibody *HI111*

10110040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11a Antibody *HI111*

10110041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *HI11b*

10111040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD11b Antibody *HI11b*

10111041 500 tests
EUR 918
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn

iFluor® 450 Anti-human CD5 Antibody *UCHT2*

10052040 100 tests
EUR 245
Description: R-Phrase for Dangerous Goods accord. to EU 67/548 EWG: R20, R21, R22; H-Phrases (GHS) for Dangerous Goods accord. to 1272/2008: H303, H313, H333; Symbol for Dangerous Compound accord. to EU 67/548 EWG: Xn
  1. Within the following, alizarin pink, oil red-O and toluidine blue staining have been carried out to judge the multi-lineage differentiation of zebrafish coronary heart and liver tissue-derived MSCs. Subsequently, the gene and protein expression of Oct4, Sox2 and Nanog as pluri

    -potent markers have been analyzed by RT-PCR and western blotting, respectively.

As well as, MTT assay was used for cell proliferation potential and inhabitants doubling time (PDT) evaluation. Additionally, the growing older of cells was investigated by β-galactosidase exercise assay. The outcomes confirmed that, like different MSCs, zebrafish coronary heart and liver tissue-derived MSCs have been constructive for mesenchymal, adverse for hematopoietic markers and expressed pluripotent markers Oct4, Sox2 and Nanog. Furthermore, these cells have been differentiated to osteocyte, adipocyte, and chondrocyte lineages following directed differentiation. It was discovered that PDT of zebrafish coronary heart and liver tissue-derived MSCs have been 50.67 and 46.61 h, respectively.

These cells had considerably extra speedy progress on day 4. Our outcomes present that zebrafish coronary heart and liver tissue-derived MSCs exhibited typical MSC traits together with fibroblast morphology, multi-lineage differentiation capability, pluriefficiency potential and expression of a typical set of basic MSC floor markers.