James

Validity evidence for an instrument for cognitive load for virtual didactic sessions

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Background: COVID necessitated the shift to virtual resident instruction. The challenge of learning via virtual modalities has the potential to increase cognitive load. It is important for educators to reduce cognitive load to optimize learning, yet there are few available tools to measure cognitive load. The objective of this study is to identify and provide validity evidence following Messicks’ framework for an instrument to evaluate cognitive load in virtual emergency medicine didactic sessions.
Methods: This study followed Messicks’ framework for validity including content, response process, internal structure, and relationship to other variables. Content validity evidence included: (1) engagement of reference librarian and literature review of existing instruments; (2) engagement of experts in cognitive load, and relevant stakeholders to review the literature and choose an instrument appropriate to measure cognitive load in EM didactic presentations. Response process validity was gathered using the format and anchors of instruments with previous validity evidence and piloting amongst the author group. A lecture was provided by one faculty to four residency programs via ZoomTM. Afterwards, residents completed the cognitive load instrument. Descriptive statistics were collected; Cronbach’s alpha assessed internal consistency of the instrument; and correlation for relationship to other variables (quality of lecture).
Results: The 10-item Leppink Cognitive Load instrument was selected with attention to content and response process validity evidence. Internal structure of the instrument was good (Cronbach’s alpha = 0.80). Subscales performed well-intrinsic load (α = 0.96, excellent), extrinsic load (α = 0.89, good), and germane load (α = 0.97, excellent). Five of the items were correlated with overall quality of lecture (p < 0.05).
Conclusions: The 10-item Cognitive Load instrument demonstrated good validity evidence to measure cognitive load and the subdomains of intrinsic, extraneous, and germane load. This instrument can be https://biodas.org/ used to provide feedback to presenters to improve the cognitive load of their presentations.

Assessment of the influence of gluten quality on highland barley dough sheet quality by different instruments

  • This study was to compare the results of texture analyzer with those of farinograph and extensograph and determine whether texture analyzer could be used to evaluate the processing quality of highland barley flour (HBF) dough sheet. The farinograph and extensograph tests were used to determine the reconstituted flour properties, a texture analyzer was applied to measure the tensile strength of HBF dough sheet, and the content of glutenin macropolymer (GMP), free sulfhydryl (-SH) and secondary structure of protein and microstructure in HBF dough sheet were investigated. Furthermore, correlations between these parameters were determined by regression analysis and Pearson correlation coefficient.
  • It was suggested that the reconstituted flours with a higher gluten index showed a higher farinograph quality number (FQN) and greater maximum resistance to extension (Rm ). HBF dough sheets with higher gluten index possessed higher GMP and lower free -SH contents, a more ordered secondary structure of protein, resulting in a more compact gluten network and a stronger tensile strength (TS).
  • The regression and correlation analysis showed that TS was positively correlated with FQN and Rm . In addition, it was significantly correlated with the content of GMP, -SH, secondary structure of protein and gluten network. It was concluded that texture analyzer could be an alternative approach to evaluate the processing quality of HBF dough sheet. Moreover, the gluten index of flours could be used to predict the processing quality of HBF dough sheet. This article is protected by copyright. All rights reserved.

Development of an Instrument to Assess the Stability of Cementless Femoral Implants Using Vibration Analysis During Total Hip Arthroplasty

Objective: The level of primary implant fixation in cementless total hip arthroplasty is a key factor for the longevity of the implant. Vibration-based methods show promise for providing quantitative information to help surgeons monitor implant fixation intraoperatively. A thorough understanding of what is driving these changes in vibrational behavior is important for further development and improvement of these methods. Additionally, an instrument must be designed to enable surgeons to leverage these methods. This study addresses both of these issues.
Method: An augmented system approach was used to develop an instrument that improves the sensitivity of the vibrational method and enables the implementation of the necessary excitation and measurement equipment. The augmented system approach took into account the dynamics of the existing bone-implant system and its interaction with the added instrument.
Results: Two instrument designs are proposed, accompanied by a convergence-based method to determine the insertion endpoint. The modal strain energy density distribution was shown to affect the vibrational sensitivity to contact changes in certain areas.
Conclusion: The augmented system approach led to an instrument design that improved the sensitivity to changes in the proximal region of the combined bone-implant-instrument system. This fact was confirmed both in silico and in vitro. Clinical Impact: The presented method and instruments address practical intraoperative challenges and provide perspective to objectively support the surgeon’s decision-making process, which will ensure optimal patient treatment.

Validation of the PAM-13 instrument in the Hungarian general population 40 years old and above

Background: Patient activation comprises the skills, knowledge and motivation necessary for patients’ effective contribution to their care. We adapted and validated the 13-item Patient Activation Measure (PAM-13) in the ≥ 40 years old Hungarian general population.
Methods: A cross-sectional web survey was conducted among 900 respondents selected from an online panel via quota sampling. After 10 days, the survey was repeated on 100 respondents. The distribution, internal consistency, test-retest reliability, factor structure, convergent, discriminant and known-groups validity of PAM-13 were assessed according to the COSMIN guidelines.
Results: The sample comprised 779 respondents. Mean (± SD) age was 60.4 ± 10.6 years, 54% were female and 67% had chronic illness. Mean (± SD) PAM-13 score was 60.6 ± 10.0. We found good internal consistency (Cronbach alpha: 0.77), moderate test-retest reliability (ICC: 0.62; n = 75), a single-factor structure and good content validity: PAM-13 showed moderate correlation with the eHealth Literacy Scale (r = 0.40), and no correlation with age (r = 0.02), education (r = 0.04) or income (ρ = 0.04). Higher PAM-13 scores were associated with fewer lifestyle risks (p < 0.001), more frequent health information seeking (p < 0.001), participation in patient education (p = 0.018) and various online health-related behaviours. When controlling for health literacy, sociodemographic factors and health status, the association of higher PAM-13 scores with overall fewer lifestyle risks, normal body mass index, physical activity and adequate diet remained significant. Similar properties were observed in the subgroup of participants with chronic morbidity, but not in the age group 65+.
Conclusion: PAM-13 demonstrated good validity in the general population. Its properties in clinical populations and the elderly as well as responsiveness to interventions warrant further research.

SmartBlock 24 x 1.5ml Thermoblock for ThermoMixer and ThermoStat instruments
E5360000038 Scientific Laboratory Supplies EACH 675 EUR

SmartBlock 24 x 0.5ml Thermoblock for ThermoMixer and ThermoStat instruments
E5361000031 Scientific Laboratory Supplies EACH 675 EUR

SmartBlock 24 x 2.0ml Thermoblock for ThermoMixer and ThermoStat instruments
E5362000035 Scientific Laboratory Supplies EACH 689.36 EUR

SmartBlock 4 x 50ml Thermoblock for ThermoMixer and ThermoStat instruments
E5365000028 Scientific Laboratory Supplies EACH 625.2 EUR

SmartBlock 8 x 15ml Thermoblock for ThermoMixer and ThermoStat instruments
E5366000021 Scientific Laboratory Supplies EACH 689.36 EUR

TIP 300UL CONDUCTIVITY - TYPE QIAGEN AND DIAGNOSTIC INSTRUMENTS,NONSTERILE,96/2304
QT-300-CBK-R CORNING 2304/pk 282 EUR

20UL CLEAR TIPS FOR PACKARDS PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS.
PK-20-R CORNING 960/pk 302.4 EUR

200UL CLEAR TIPS FOR PACKARDS PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS.
PK-200-R CORNING 960/pk 342 EUR

50UL CLEAR TIPS FOR PACKARDS PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS.
PK-50-R CORNING 960/pk 298.8 EUR

SmartBlock 12mm Thermoblock for 24 x 11-11.9mm dia tubes for ThermoMixer and ThermoStat instruments
E5364000024 Scientific Laboratory Supplies EACH 838.2 EUR

200UL CLEAR MAXYMUM RECOVERY TIPS FOR PACKARD"S PLATE TRACK, EVOLUTION AND MINI TRACK INSTRUMENTS
PK-200-L-R CORNING 960/pk 360 EUR

SmartBlock PCR 96 Thermoblock for 96 well PCR plates inc Lid for ThermoMixer and ThermoStat instruments
E5306000006 Scientific Laboratory Supplies EACH 838.2 EUR

SmartBlock PCR 384 Thermoblock for 384 well PCR plates inc Lid for ThermoMixer and ThermoStat instruments
E5307000000 Scientific Laboratory Supplies EACH 838.2 EUR

SmartBlock plates Thermoblock for MTP and Deepwell plates inc Lid for ThermoMixer and Thermo tat instruments
E5363000039 Scientific Laboratory Supplies EACH 838.2 EUR

SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK
AM-96-SEPTA-3100 CORNING 10/pk 639.6 EUR

AnaPrep 12 Instrument
Z1321001 Biochain 1 unit 27916.8 EUR

AGS8830-8 PCR instrument
AGS8830 Daan Gene 1 unit/set Ask for price

Nucleic Acid Instrument
Smart-32 Daan Gene 1 unit/set Ask for price

Nucleic Acid Instrument
Swift-96 Daan Gene 1 unit/set Ask for price

BioDrop Instrument Cover
SPE6052 Scientific Laboratory Supplies EACH 72 EUR

BioDrop PC Instrument Cover
SPE6054 Scientific Laboratory Supplies EACH 111.6 EUR

Instrument Check Standard 8
CALMIX8-100 Scientific Laboratory Supplies 125ML 222 EUR

Differential gene expression and chemical patterns of an intertidal crab inhabiting a polluted port and an adjacent marine protected area

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The acquisition of data to safeguard marine protected areas located close to ports is important in order to develop plans that allow effective protection from pollution as well as sustainable development of the port. The area Secche della Meloria is a Marine Protected Area (MPA-MEL) three miles from Livorno Harbour (LH), which is characterized by a long history of pollution. Here we studied the bioaccumulation and transcriptomic patterns of the marbled crab, Pachygrapsus marmoratus (Fabricius, 1787) (Crustacea; Brachyura, Grapsidae), inhabiting the two selected sites.
Results showed that the two crab populations are significantly different in their chemical composition of trace elements and Polyciclic Aromatic Hydrocarbons (PAHs), and gene expression patterns (1280 DEGs). Enrichment analysis indicated that crabs at LH had the highest stress response genes, and they were associated with higher levels of bioaccumulation detected in body tissues. We are confident that the significant differential gene expression profiles observed between crabs, characterized by https://biodas.org/ significant chemical differences, is associated with responses to contaminant exposure.

Comparison of Perioperative Outcomes Between Single-Port and Multi-Port Robotic Adrenalectomy

Background: Single-port (SP) robotic surgery has been utilized in several surgical procedures. We aim to describe our institution’s approach and perioperative experience with SP robotic adrenalectomy and compare it to the traditional multi-port (MP) approach.
Methods: We retrospectively reviewed all patients who underwent robotic adrenalectomy by a single surgeon between March 2019 and March 2020. Patient demographic, perioperative factors, and pathologic outcomes were recorded and analyzed using t-tests, chi-square, or Fisher’s exact tests.
Results: Thirty-six patients underwent SP (n = 11) and MP (n = 25) robotic adrenalectomy. Age, body mass index, gender, operative time, major Clavien-Dindo complications, and margin status showed no differences. Patients undergoing SP adrenalectomy had a lower estimated blood loss (18.1 ± 13.0 vs 65.6 ± 95.0 cc, P = .02) and smaller lesion size (2.8 ± 1.3 vs 4.1 ± 1.8 cm, P = .04) compared to those undergoing MP.
Conclusions: SP adrenalectomy appears to be a feasible approach in select adrenal masses. Further studies are needed to establish its safety and cost effectiveness.

Analysis of ocular injury 1-year outcome in survivors of Beirut Port ammonium nitrate blast

Purpose: Ascertain the 1-year outcome of patients who sustained open eye injuries from the Beirut Port ammonium nitrate (AN) explosion, one of the most powerful non-nuclear explosions in history.
Methods: Retrospective chart review of the operated eyes in 2 major eye hospitals.
Results: Out of 42 patients with open globe injury that was originally sutured, 29 patients (34 eyes) were followed at the 1-year mark. The initial vision in logMAR (mean ± SD) was 2.93 ± 0.87 (hand motion equivalent) and the final vision was 1.80 ± 1.47 (counting finger 2 m equivalent). No light perception (NLP) vision was noted in 12 eyes on presentation and 10 eyes remained so, while 2 eyes reached light perception (LP) vision. Eight eyes had an intraoperative expulsive choroidal hemorrhage (7 NLP and 1 LP both pre- and postoperatively), and 6 of the 8 developed phthisis. All eyes that developed phthisis had NLP preoperatively and postoperatively. Ocular Trauma Score (OTS) correlated inversely with both initial and final vision (p < 0.001). Zone of injury inversely correlated with initial vision (p = 0.02) and positively with final vision (p < 0.001). Final vision was significantly worse in zone 3 vs. zones 1 and 2 (3.2 ± 0.5) vs. 0.9 ± 1.1) (p < 0.001) injuries, as was the initial vision (3.3 ± 0.5 vs. 2.7 ± 0.8; p = 0.002).
Conclusion: The OTS, which provides prognostic information for serious ocular trauma, also yields valuable prognostic information for AN-associated ocular injuries. Expulsive choroidal hemorrhage and NLP vision at presentation remain very poor prognostic signs.

Da Vinci SP Single-Port Robotic Surgery in Gynecologic Tumors: Single Surgeon’s Initial Experience with 100 Cases

Purpose: To report preliminary experience of single-port robotic surgery using the da Vinci SP surgical system in gynecologic tumors.
Materials and methods: This was a retrospective study on 100 consecutive patients who underwent da Vinci SP single-port robotic surgery between November 2018 and January 2021. All procedures were performed by an experienced gynecologic surgeon using a single 2.5-cm umbilical incision.
Results: Of the 100 cases, the procedures included myomectomy (n=76), hysterectomy (n=2), endometrial cancer surgical staging (n=14), radical hysterectomy (n=3), radical trachelectomy (n=3), and ovarian cystectomy (n=2). None of the cases was converted to robotic multiport or open surgery. The median docking time was 5.0 minutes [interquartile range (IQR), 3.0-7.0], the median console time was 107.5 minutes (IQR, 78.7-155.8), and the median total operation time was 250.0 minutes (IQR, 215.0-310.0). The median estimated blood loss was 50.0 mL (IQR, 30.0-100.0), and the median change in hemoglobin level was 0.8 g/dL (IQR, 0.3-1.3). The median pain scores rated on a numerical rating scale immediately after and at 6, 12, and 24 hours after surgery were 5, 2, 2, and 2, respectively. The mean duration of postoperative hospitalization was 2.8 days.
Conclusion: Da Vinci SP single-port robotic surgery was successfully performed in various gynecologic tumors without significant complications. Therefore, this surgical system could be applied in patients who want precise gynecologic surgery while minimizing surgical incision.

Anxiety, Depression and PTSD in Children and Adolescents following the Beirut Port Explosion

Background: On August 4, 2020, Beirut’s port experienced one of the strongest non-nuclear explosions in history, killing approximately 200 people, displacing 300,000 persons, and injuring more than 1000 children.
Methods: An online anonymous survey assessed the prevalence of probable mental health disorders (MHDs) and impact of blast-related and other factors controlling for sociodemographics in 801 children aged 8 to 17 years old.
Results: About two thirds (64%) were screened positive for probable anxiety using the Screen for Childhood Anxiety Related Disorder, 52% for probable PTSD using CRIES-13, and 33% for probable depression using the Mood and Feelings Questionnaire (MFQ). Children who resided farthest way from the explosion site or were not in Beirut during blast had a significantly lower odds of anxiety and PTSD. Children who sustained any physical injury (vs. none) or witnessed casualties (vs. not) were at higher odds for PTSD. Children of parents who reported that their homes sustained minor damages (vs. no damages at all) were at higher odds for anxiety and PTSD, and temporary displacement (vs. none) increased odds of PTSD only. Poorer perceived economic status, poorer academic performance, having a family member injured in the blast, and prior mental health care seeking were associated with higher odds for all MHDs.
Conclusion: Our study, the only one to document the mental health impact of the Beirut Port explosion on children, highlights the critical need for an emergency mental health response, prioritizing disadvantaged communities and children with prior mental health problems.

8-Port Manifolds
9621 Genesee Scientific 10 Manifolds/Unit 138 EUR

NBS 6 Port Gassing Manifold
SHA1124 Scientific Laboratory Supplies EACH 955.2 EUR

Kinesis Manifold Body 6 Port 1/8in
CHR3821 Scientific Laboratory Supplies EACH 310.8 EUR

Kinesis Manifold Assy 6 Port 1/16in
CHR3817 Scientific Laboratory Supplies EACH 338.4 EUR

Kinesis Manifold Body 7 Port; 1/16; PEEK
CHR3813 Scientific Laboratory Supplies EACH 147.6 EUR

Kinesis Manifold Assembly 7 Port; 1/16; PEEK
CHR3811 Scientific Laboratory Supplies EACH 153.6 EUR

Kinesis Manifold Assembly 7 Port; 1/8; PEEK
CHR3815 Scientific Laboratory Supplies EACH 152.4 EUR

Kinesis Manifold Assembly 6 Port; 1/8; PEEK
CHR3819 Scientific Laboratory Supplies EACH 140.4 EUR

Kinesis Manifold Assembly 5 Port; 1/16; PEEK
CHR3823 Scientific Laboratory Supplies EACH 172.8 EUR

Kinesis Manifold Assembly 5 Port; 1/16; PEEK
CHR3825 Scientific Laboratory Supplies EACH 196.8 EUR

Kinesis Manifold Assembly 5 Port; 1/8; PEEK
CHR3827 Scientific Laboratory Supplies EACH 128.4 EUR

Kinesis Manifold Assembly 9 Port; 1/8; PEEK
CHR3831 Scientific Laboratory Supplies EACH 199.2 EUR

Kinesis Manifold Assembly 9 Port; 1/16; PEEK
CHR3833 Scientific Laboratory Supplies EACH 232.8 EUR

SmartWasher™ 96 Microplate Washer for 96 Well Plates, manifolds not included, 115V
MW9600 Benchmark Scientific 1 each 5009.3 EUR

SmartWasher™ 96 Microplate Washer for 96 Well Plates, manifolds not included, 230V
MW9600-E Benchmark Scientific 1 each 5009.3 EUR

Kinesis Manifold Assembly 7 Port; 1/16; PEEK; 0.02 (0.5mm) Bore
CHR3829 Scientific Laboratory Supplies EACH 184.8 EUR

J Young Gpe Dual Bank Manifold With JY Valves 3 Port 300mm Approx Width
NMR1393 Scientific Laboratory Supplies EACH 741 EUR

J Young Gpe Dual Bank Manifold With JY Valves 4 Port 400mm Approx Width
NMR1395 Scientific Laboratory Supplies EACH 905.16 EUR

J Young Gpe Dual Bank Manifold With JY Valves 5 Port 500mm Approx Width
NMR1397 Scientific Laboratory Supplies EACH 1078.44 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY Valves And Vacuum Gauge Port 3 Port 300mm Approx Width
NMR1381 Scientific Laboratory Supplies EACH 449.16 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY Valves And Vacuum Gauge Port 4 Port 400mm Approx Width
NMR1383 Scientific Laboratory Supplies EACH 538.08 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY Valves And Vacuum Gauge Port 5 Port 500mm Approx Width
NMR1385 Scientific Laboratory Supplies EACH 628.14 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY High Performance Valves And Vacuum Gauge Port 3 Port 300mm Approx Width
NMR1382 Scientific Laboratory Supplies EACH 499.32 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY High Performance Valves And Vacuum Gauge Port 4 Port 400mm Approx Width
NMR1384 Scientific Laboratory Supplies EACH 606.48 EUR

J Young General Purpose Single Bank Vacuum Manifold With JY High Performance Valves And Vacuum Gauge Port 5 Port 500mm Approx Width
NMR1386 Scientific Laboratory Supplies EACH 713.64 EUR

J Young Gpe Dual Bank Manifold With JY High Performance Valves 3 Port 300mm Approx Width
NMR1394 Scientific Laboratory Supplies EACH 784.32 EUR

J Young Gpe Dual Bank Manifold With JY High Performance Valves 4 Port 400mm Approx Width
NMR1396 Scientific Laboratory Supplies EACH 970.14 EUR

J Young Gpe Dual Bank Manifold With JY High Performance Valves 5 Port 500mm Approx Width
NMR1398 Scientific Laboratory Supplies EACH 1161.66 EUR

Comparative thermoresistance of two biological indicators for monitoring steam autoclaves. 3. Comparison performed at 121 degrees C in a hospital prevacuum steam sterilizer

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According to Pharmacopoea Nordica, steam autoclaves should be regularly monitored by a specific Swedish preparation of Bacillus stearothermophilus spores. If another biological indicator (BI) is used for such a control, it should first be calibrated against the Swedish BI (SBI) and the two BIs should be equally thermoresistant. Attest No. 1262 BI (ABI) has previously been shown to be more thermoresistant than the SBI at 134 degrees C, saturated steam. The purpose of the present study was to compare the thermoresistance of the SBI and the ABI at 121 degrees C, saturated steam and prevacuum. Seven hundred and twenty units of each BI were heat-exposed in an Emmer 760 litre prevacuum, pressure-pulsing steam autoclave.
After prevacuum with steam injection (manual or automatic preconditioning), the following incremental heat exposure times were used in triplicate (20 simultaneously tested units of each BI in each cycle) according to a randomized scheme: 5, 6 1/2, 8, 9 1/2, 11, 12 1/2, 14 and 15 min. The intra-chamber pressure and temperature were continuously monitored throughout the test and equilibration cycles.
The heat-exposed BI units were cultivated and read as recommended by the manufacturers. SBI and ABI showed a survival-time of 8 min and 11 min respectively, and a kill-time between 14 min and 15 min for both BIs. Thus, the ABI had the narrower survival-kill window. Probit analysis testing of the results showed that the difference in thermoresistance, at 121 degrees C, saturated steam and https://biodas.org/ prevacuum between Attest No. 1262 BI and the Swedish BI mentioned in Pharmacopea Nordica was not statistically significant.

Comparative thermoresistance of two biological indicators for monitoring steam autoclaves. 2. Comparison performed at 134 degrees C in a hospital prevacuum steam sterilizer

The thermoresistance of various lots of two biological indicators (BIs) for steam sterilization control, a Scandinavian BI (SBI) and the Attest BI (ABI), were compared during sterilization cycles in a hospital prevacuum (pressure-pulsing) steam autoclave at 134 degrees C, saturated steam. ABI No. 1242, ABI No. 1262 (its replacement) and incremental heat exposure times between 0 s and 180 s were used. The intrachamber temperature and pressure were continuously measured and monitored throughout the sterilization cycles.
The results showed that both of the ABIs were more thermoresistant than the SBI, giving 33.1% (ABI No. 1242), 18.9% (ABI No. 1262), and 0% (SBI) autoclave survivors. Because the time needed to reach 134 degrees C (preconditioning time) increased as the day progressed, and varied from day to day, correlation between individual incremental heat exposure times and the number of surviving BI units was not possible. Standardized test conditions are necessary for a true comparison of BIs.

Ozone: A Novel Sterilizer for Personal Protective Equipment

Objective: Personal protective equipment (PPE) is urgently sought during public health crises. It is necessary for the safety of both the patient and the healthcare professional. Yet during the recent COVID-19 pandemic, PPE scarcity in many countries, including the United States, has impacted the level of care for patients and the safety of healthcare personnel. Additionally, the implementation of mandatory mask mandates for the general public in many countries forced individuals to either reuse PPE, which can contribute to poor hygiene, or buy PPE in bulk and thereby contribute to the scarcity of PPE. In this study, we investigate the possibility of using a cost-effective ozone sterilization unit on contaminated N95 masks as an alternative to current sterilization methods.
Method: This protocol examined ozone’s ability to decontaminate N95 mask fabric that was exposed to a surrogate virus (Escherichia coli bacteriophage MS2). Once the sterilization unit achieves an ozone concentration of ~30 ppm, a 60-minute or 120-minute sterilization cycle commences. Following the sterilization cycle, we investigated the amount of viable virus on the slide using a viral plaque assay and compared it to a non-sterilized, control slide. Furthermore, we carried out trials to investigate the safety of an ozone sterilization device, by measuring the levels of ozone exposure that individuals may experience when operating the sterilization unit post-cycle.
Results: We showed that a 120-minute sterilization cycle at ~30 ppm achieves a 3-log reduction in viral activity, thereby complying with industry and U.S. Food and Drug Administration (FDA) standards. Further, we demonstrated that when following our protocol, the ozone exposure levels for a simple sterilization unit to be used at home complied with federal and industry standards.
Conclusion: Ozone may have the potential to decontaminate masks and other PPE.

Monitoring the Effective Sterilization of Low-Temperature Hydrogen Peroxide Gas Plasma Sterilizers in 58 Hospitals – 22 PLADs, China, June 2015-December 2019

What is already known on this topic?: Hydrogen peroxide sterilizeation is widely used for luminal devices. However, the low penetrability of the sterilant is of major concern.
What is added by this report?: This report investigated the effective sterilization of low-temperature hydrogen peroxide gas plasma sterilizers and compared the applicability of different biological monitoring methods based on medical luminal devices.
What are the implications for public health practice?: It is recommended to use a biological process challenge device for monitoring the sterilization of luminal devices with low-temperature hydrogen peroxide gas plasma sterilizers.

Portable sterilizer with microbe content detection device

  • Background: Infectious diseases, such as the latest COVID pandemic, caused by microorganisms like bacteria and virus, wreak havoc shaking human civilization with its rapid infection rate, and high number of mortalities. In case of a contagious disease, the virus can survive on any surface over a period of time and can be transferred to the human host through touching those surfaces unknowingly. Cleaning those possible surfaces to which these microorganisms can cling onto is one of the major ways to curb the spread. The aim of this study was to design a sterilizer which can clean such surfaces of daily used items easily within a certain period of time and can assess the cleaning efficacy by estimating the presence of microbes before and after sanitization.
  • Method development: To achieve this goal, we propose a portable sterilization unit that contains a sterilization chamber fitted with a microbe content detector. The sterilization chamber will cleanse the surfaces off the microbes using ultraviolet radiation. The chamber can be portable and at the same time big enough to accommodate items of daily use, like watch, wallet, clothes, utensils to even foods for single-house application. The microbe content detector will detect the success of the sterilization procedure by examining the time-lapse laser speckle images captured by a high-speed camera by mean of image processing algorithm, such that the user can determine whether further sterilization is required.
  • Conclusions: Microbe content detection device associated with the conventional sterilization procedure will give an assessment of the effectiveness of the sterilization. Successful implementation of sterilization for a wide variety of items of everyday use aided with microbe content detection technique is first of its kind and should be an effective tool for use in large communities, offices and public places for effective sterilization to help fight against the spread of infectious diseases.

Integrated Pre-heated 9kw boiler for Rodwell autoclaves for Rodwell autoclaves
AUT1379 Scientific Laboratory Supplies EACH 5214.57 EUR

Printer for Media Autoclaves
AUT4519 Scientific Laboratory Supplies EACH 445.21 EUR

Automatic Fill for Rodwell autoclaves
AUT1349 Scientific Laboratory Supplies EACH 1450.8 EUR

Shelf Support for Rodwell autoclaves
AUT1374 Scientific Laboratory Supplies EACH 946.8 EUR

Condensate removal for Rodwell autoclaves
AUT1381 Scientific Laboratory Supplies EACH 1357.2 EUR

Condensate Collector for Rodwell autoclaves
AUT2019 Scientific Laboratory Supplies EACH 349.2 EUR

Printer 2 channel for Rodwell autoclaves
AUT1272 Scientific Laboratory Supplies EACH 1228.8 EUR

Oil Free Compressor for Rodwell autoclaves
AUT1376 Scientific Laboratory Supplies EACH 2601.6 EUR

Fan assisted cooling for Rodwell autoclaves
AUT1356 Scientific Laboratory Supplies EACH 1309.52 EUR

Fan Assisted Cooling for Rodwell autoclaves
AUT1357 Scientific Laboratory Supplies EACH 585.6 EUR

Drain Line Condenser for Rodwell autoclaves
AUT1358 Scientific Laboratory Supplies EACH 1350 EUR

Under Chamber Boiler for Rodwell autoclaves
AUT1377 Scientific Laboratory Supplies EACH 4308.48 EUR

Loading step for Ensign for Rodwell autoclaves
AUT1350 Scientific Laboratory Supplies EACH 654 EUR

Discard System for Genesis for Rodwell autoclaves
AUT2000 Scientific Laboratory Supplies EACH 1030.8 EUR

2 channel + pressure printer for Rodwell autoclaves
AUT1274 Scientific Laboratory Supplies EACH 1712.4 EUR

Automatic FillDrain for Clean Steam for Rodwell autoclaves
AUT1351 Scientific Laboratory Supplies EACH 2328 EUR

Discard system for Rodwell Ambassador 100 and Gemini autoclaves (per chamber)
AUT2007 Scientific Laboratory Supplies EACH 1568.4 EUR

Sovereign Autoclave 200E
AUT0507 Scientific Laboratory Supplies EACH 27600 EUR

Sovereign Autoclave 250E
AUT0509 Scientific Laboratory Supplies EACH 27600 EUR

Prestige Autoclave 2100 Extended
AUT4502 Scientific Laboratory Supplies EACH 1504.8 EUR

Prestige Autoclave 2100 Extended+
AUT4504 Scientific Laboratory Supplies EACH 2434.94 EUR

Floor Standing Autoclave - 120L
AUT5000 Scientific Laboratory Supplies EACH 32806.8 EUR

Floor Standing Autoclave - 153L
AUT5002 Scientific Laboratory Supplies EACH 36426 EUR

Floor Standing Autoclave - 247L
AUT5004 Scientific Laboratory Supplies EACH 40245.6 EUR

Floor Standing Autoclave - 290L
AUT5006 Scientific Laboratory Supplies EACH 42969.6 EUR

Floor Standing Autoclave - 344L
AUT5008 Scientific Laboratory Supplies EACH 44948.4 EUR