Differential gene expression and chemical patterns of an intertidal crab inhabiting a polluted port and an adjacent marine protected area

The acquisition of data to safeguard marine protected areas located close to ports is important in order to develop plans that allow effective protection from pollution as well as sustainable development of the port. The area Secche della Meloria is a Marine Protected Area (MPA-MEL) three miles from Livorno Harbour (LH), which is characterized by a long history of pollution. Here we studied the bioaccumulation and transcriptomic patterns of the marbled crab, Pachygrapsus marmoratus (Fabricius, 1787) (Crustacea; Brachyura, Grapsidae), inhabiting the two selected sites.
Results showed that the two crab populations are significantly different in their chemical composition of trace elements and Polyciclic Aromatic Hydrocarbons (PAHs), and gene expression patterns (1280 DEGs). Enrichment analysis indicated that crabs at LH had the highest stress response genes, and they were associated with higher levels of bioaccumulation detected in body tissues. We are confident that the significant differential gene expression profiles observed between crabs, characterized by https://biodas.org/ significant chemical differences, is associated with responses to contaminant exposure.

Comparison of Perioperative Outcomes Between Single-Port and Multi-Port Robotic Adrenalectomy

Background: Single-port (SP) robotic surgery has been utilized in several surgical procedures. We aim to describe our institution’s approach and perioperative experience with SP robotic adrenalectomy and compare it to the traditional multi-port (MP) approach.
Methods: We retrospectively reviewed all patients who underwent robotic adrenalectomy by a single surgeon between March 2019 and March 2020. Patient demographic, perioperative factors, and pathologic outcomes were recorded and analyzed using t-tests, chi-square, or Fisher’s exact tests.
Results: Thirty-six patients underwent SP (n = 11) and MP (n = 25) robotic adrenalectomy. Age, body mass index, gender, operative time, major Clavien-Dindo complications, and margin status showed no differences. Patients undergoing SP adrenalectomy had a lower estimated blood loss (18.1 ± 13.0 vs 65.6 ± 95.0 cc, P = .02) and smaller lesion size (2.8 ± 1.3 vs 4.1 ± 1.8 cm, P = .04) compared to those undergoing MP.
Conclusions: SP adrenalectomy appears to be a feasible approach in select adrenal masses. Further studies are needed to establish its safety and cost effectiveness.

Analysis of ocular injury 1-year outcome in survivors of Beirut Port ammonium nitrate blast

Purpose: Ascertain the 1-year outcome of patients who sustained open eye injuries from the Beirut Port ammonium nitrate (AN) explosion, one of the most powerful non-nuclear explosions in history.
Methods: Retrospective chart review of the operated eyes in 2 major eye hospitals.
Results: Out of 42 patients with open globe injury that was originally sutured, 29 patients (34 eyes) were followed at the 1-year mark. The initial vision in logMAR (mean ± SD) was 2.93 ± 0.87 (hand motion equivalent) and the final vision was 1.80 ± 1.47 (counting finger 2 m equivalent). No light perception (NLP) vision was noted in 12 eyes on presentation and 10 eyes remained so, while 2 eyes reached light perception (LP) vision. Eight eyes had an intraoperative expulsive choroidal hemorrhage (7 NLP and 1 LP both pre- and postoperatively), and 6 of the 8 developed phthisis. All eyes that developed phthisis had NLP preoperatively and postoperatively. Ocular Trauma Score (OTS) correlated inversely with both initial and final vision (p < 0.001). Zone of injury inversely correlated with initial vision (p = 0.02) and positively with final vision (p < 0.001). Final vision was significantly worse in zone 3 vs. zones 1 and 2 (3.2 ± 0.5) vs. 0.9 ± 1.1) (p < 0.001) injuries, as was the initial vision (3.3 ± 0.5 vs. 2.7 ± 0.8; p = 0.002).
Conclusion: The OTS, which provides prognostic information for serious ocular trauma, also yields valuable prognostic information for AN-associated ocular injuries. Expulsive choroidal hemorrhage and NLP vision at presentation remain very poor prognostic signs.

Da Vinci SP Single-Port Robotic Surgery in Gynecologic Tumors: Single Surgeon’s Initial Experience with 100 Cases

Purpose: To report preliminary experience of single-port robotic surgery using the da Vinci SP surgical system in gynecologic tumors.
Materials and methods: This was a retrospective study on 100 consecutive patients who underwent da Vinci SP single-port robotic surgery between November 2018 and January 2021. All procedures were performed by an experienced gynecologic surgeon using a single 2.5-cm umbilical incision.
Results: Of the 100 cases, the procedures included myomectomy (n=76), hysterectomy (n=2), endometrial cancer surgical staging (n=14), radical hysterectomy (n=3), radical trachelectomy (n=3), and ovarian cystectomy (n=2). None of the cases was converted to robotic multiport or open surgery. The median docking time was 5.0 minutes [interquartile range (IQR), 3.0-7.0], the median console time was 107.5 minutes (IQR, 78.7-155.8), and the median total operation time was 250.0 minutes (IQR, 215.0-310.0). The median estimated blood loss was 50.0 mL (IQR, 30.0-100.0), and the median change in hemoglobin level was 0.8 g/dL (IQR, 0.3-1.3). The median pain scores rated on a numerical rating scale immediately after and at 6, 12, and 24 hours after surgery were 5, 2, 2, and 2, respectively. The mean duration of postoperative hospitalization was 2.8 days.
Conclusion: Da Vinci SP single-port robotic surgery was successfully performed in various gynecologic tumors without significant complications. Therefore, this surgical system could be applied in patients who want precise gynecologic surgery while minimizing surgical incision.

Anxiety, Depression and PTSD in Children and Adolescents following the Beirut Port Explosion

Background: On August 4, 2020, Beirut’s port experienced one of the strongest non-nuclear explosions in history, killing approximately 200 people, displacing 300,000 persons, and injuring more than 1000 children.
Methods: An online anonymous survey assessed the prevalence of probable mental health disorders (MHDs) and impact of blast-related and other factors controlling for sociodemographics in 801 children aged 8 to 17 years old.
Results: About two thirds (64%) were screened positive for probable anxiety using the Screen for Childhood Anxiety Related Disorder, 52% for probable PTSD using CRIES-13, and 33% for probable depression using the Mood and Feelings Questionnaire (MFQ). Children who resided farthest way from the explosion site or were not in Beirut during blast had a significantly lower odds of anxiety and PTSD. Children who sustained any physical injury (vs. none) or witnessed casualties (vs. not) were at higher odds for PTSD. Children of parents who reported that their homes sustained minor damages (vs. no damages at all) were at higher odds for anxiety and PTSD, and temporary displacement (vs. none) increased odds of PTSD only. Poorer perceived economic status, poorer academic performance, having a family member injured in the blast, and prior mental health care seeking were associated with higher odds for all MHDs.
Conclusion: Our study, the only one to document the mental health impact of the Beirut Port explosion on children, highlights the critical need for an emergency mental health response, prioritizing disadvantaged communities and children with prior mental health problems.

8-Port Manifolds

9621 Genesee Scientific 10 Manifolds/Unit 138 EUR

Y-Manifolds 5/pk

1292212 Atto 3unit 316.8 EUR

PORT-CCR1 Plasmid

PVT15911 Lifescience Market 2 ug 390 EUR

18mm dia access port

INC2188 Scientific Laboratory Supplies EACH 217.74 EUR

Kinesis Rheodyne Needle Port

CHR2939 Scientific Laboratory Supplies EACH 42.18 EUR

LTE Access Port 18mm

OVE1744 Scientific Laboratory Supplies EACH 207.48 EUR

Screw Cap GL45 3 Port

1129751 Scientific Laboratory Supplies PK2 69.54 EUR

Screw Cap GL45 4 Port

1129812 Scientific Laboratory Supplies PK2 76.38 EUR

NBS 6 Port Gassing Manifold

SHA1124 Scientific Laboratory Supplies EACH 840.18 EUR

Closure Nalgene PP 2 port x38in

ASP2091 Scientific Laboratory Supplies EACH 302.1 EUR

Screw Cap GL45 PP 2 Port

1129750 Scientific Laboratory Supplies PK2 64.98 EUR

Kinesis Injection Port Adapter; 10-32

CHR3629 Scientific Laboratory Supplies EACH 44.46 EUR

Kinesis NanoPort Gasket Flat Bottom Port

CHR3741 Scientific Laboratory Supplies EACH 28.5 EUR

DURAN 1-port connector cap GL45

BOT2274 Scientific Laboratory Supplies EACH 247.38 EUR

DURAN 2-port connector cap GL45

BOT2276 Scientific Laboratory Supplies EACH 251.94 EUR

DURAN 3-port connector cap GL45

BOT2278 Scientific Laboratory Supplies EACH 251.94 EUR


ZLXGCLNKT Scientific Laboratory Supplies EACH 445.74 EUR


ZR00CLNKT Scientific Laboratory Supplies EACH 744.42 EUR

wide horizontal unit 23.5x40 cm ex. port

EHS3620-SYS Consort ea 1132.8 EUR

Closure Nalgene PP 3 port x 14

ASP2092 Scientific Laboratory Supplies EACH 438.9 EUR

Closure Nalgene PP 3 port x 38i

ASP2093 Scientific Laboratory Supplies EACH 422.94 EUR

Kinesis Manifold Assy 6 Port 1/16in

CHR3817 Scientific Laboratory Supplies EACH 281.58 EUR

Kinesis Manifold Body 6 Port 1/8in

CHR3821 Scientific Laboratory Supplies EACH 258.78 EUR

SmartWasher™ 96 Microplate Washer for 96 Well Plates, manifolds not included, 115V

MW9600 Benchmark Scientific 1 each 5009.3 EUR

SmartWasher™ 96 Microplate Washer for 96 Well Plates, manifolds not included, 230V

MW9600-E Benchmark Scientific 1 each 5009.3 EUR

Kinesis PCB for TitanHT 2 Pos 6 Port

CHR2899 Scientific Laboratory Supplies EACH 274.74 EUR

Kinesis Fitting 1/16 OD 6-40 Port

CHR3669 Scientific Laboratory Supplies EACH 22.8 EUR

Kinesis Manifold Assembly 7 Port; 1/16; PEEK

CHR3811 Scientific Laboratory Supplies EACH 124.26 EUR